Experiment: P. put PUT 10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2901 and PP_2902 are separated by 81 nucleotides PP_2902 and PP_2903 overlap by 4 nucleotides PP_2903 and PP_2904 are separated by 248 nucleotides
PP_2901: PP_2901 - Acyl-homoserine lactone acylase, at 3,297,784 to 3,300,102
_2901
PP_2902: PP_2902 - UDP-2,3-diacylglucosamine hydrolase, at 3,300,184 to 3,300,906
_2902
PP_2903: PP_2903 - peptidyl-prolyl cis-trans isomerase B (rotamase B), at 3,300,903 to 3,301,403
_2903
PP_2904: PP_2904 - Glutamine--tRNA ligase, at 3,301,652 to 3,303,355
_2904
Position (kb)
3300
3301 Strain fitness (log2 ratio)
-2
-1
0
1 at 3299.234 kb on + strand, within PP_2901 at 3299.235 kb on - strand, within PP_2901 at 3299.235 kb on - strand, within PP_2901 at 3299.290 kb on + strand, within PP_2901 at 3299.375 kb on + strand at 3299.381 kb on + strand, within PP_2901 at 3299.381 kb on + strand at 3299.382 kb on - strand at 3299.382 kb on - strand, within PP_2901 at 3299.419 kb on + strand, within PP_2901 at 3299.419 kb on + strand, within PP_2901 at 3299.419 kb on + strand, within PP_2901 at 3299.419 kb on + strand, within PP_2901 at 3299.419 kb on + strand, within PP_2901 at 3299.419 kb on + strand, within PP_2901 at 3299.420 kb on - strand, within PP_2901 at 3299.420 kb on - strand, within PP_2901 at 3299.539 kb on - strand, within PP_2901 at 3299.666 kb on + strand, within PP_2901 at 3299.772 kb on + strand, within PP_2901 at 3299.772 kb on + strand, within PP_2901 at 3299.772 kb on + strand, within PP_2901 at 3299.773 kb on - strand, within PP_2901 at 3299.809 kb on + strand, within PP_2901 at 3299.809 kb on + strand, within PP_2901 at 3299.809 kb on + strand, within PP_2901 at 3299.809 kb on + strand, within PP_2901 at 3299.810 kb on - strand, within PP_2901 at 3299.810 kb on - strand, within PP_2901 at 3299.821 kb on + strand, within PP_2901 at 3299.872 kb on + strand at 3299.872 kb on + strand at 3299.873 kb on - strand at 3300.180 kb on - strand at 3301.292 kb on - strand, within PP_2903 at 3301.292 kb on - strand, within PP_2903 at 3301.292 kb on - strand, within PP_2903 at 3301.596 kb on + strand at 3301.596 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. put PUT 10 MOI 1 remove 3,299,234 + PP_2901 0.63 +1.1 3,299,235 - PP_2901 0.63 -0.2 3,299,235 - PP_2901 0.63 +0.5 3,299,290 + PP_2901 0.65 +0.0 3,299,375 + -0.5 3,299,381 + PP_2901 0.69 -0.6 3,299,381 + +1.3 3,299,382 - -1.8 3,299,382 - PP_2901 0.69 -0.8 3,299,419 + PP_2901 0.71 +0.2 3,299,419 + PP_2901 0.71 -0.1 3,299,419 + PP_2901 0.71 +1.4 3,299,419 + PP_2901 0.71 +0.2 3,299,419 + PP_2901 0.71 -0.3 3,299,419 + PP_2901 0.71 +0.0 3,299,420 - PP_2901 0.71 +0.4 3,299,420 - PP_2901 0.71 +0.1 3,299,539 - PP_2901 0.76 +0.2 3,299,666 + PP_2901 0.81 -0.9 3,299,772 + PP_2901 0.86 -0.2 3,299,772 + PP_2901 0.86 -0.3 3,299,772 + PP_2901 0.86 +0.1 3,299,773 - PP_2901 0.86 -0.2 3,299,809 + PP_2901 0.87 +0.6 3,299,809 + PP_2901 0.87 -0.1 3,299,809 + PP_2901 0.87 -0.4 3,299,809 + PP_2901 0.87 -0.4 3,299,810 - PP_2901 0.87 -0.8 3,299,810 - PP_2901 0.87 -0.3 3,299,821 + PP_2901 0.88 -1.7 3,299,872 + +0.7 3,299,872 + +0.3 3,299,873 - -0.6 3,300,180 - +0.5 3,301,292 - PP_2903 0.78 -1.4 3,301,292 - PP_2903 0.78 -1.1 3,301,292 - PP_2903 0.78 -0.5 3,301,596 + -2.5 3,301,596 + -1.9
Or see this region's nucleotide sequence