Experiment: P. put PUT 10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1898 and PP_1899 overlap by 4 nucleotides PP_1899 and PP_1900 overlap by 4 nucleotides PP_1900 and PP_1901 are separated by 44 nucleotides PP_1901 and PP_1902 overlap by 4 nucleotides PP_1902 and PP_1903 overlap by 1 nucleotides
PP_1898: PP_1898 - MotA/TolQ/ExbB proton channel family protein, at 2,142,550 to 2,143,224
_1898
PP_1899: PP_1899 - ExbD/TolR family protein, at 2,143,221 to 2,143,655
_1899
PP_1900: PP_1900 - Tetraacyldisaccharide 4'-kinase, at 2,143,652 to 2,144,656
_1900
PP_1901: PP_1901 - conserved protein of unknown function, UPF0434 family, at 2,144,701 to 2,144,886
_1901
PP_1902: PP_1902 - 3-deoxy-manno-octulosonate cytidylyltransferase, at 2,144,883 to 2,145,647
_1902
PP_1903: PP_1903 - putative phosphotyrosine-protein phosphatase, at 2,145,647 to 2,146,111
_1903
Position (kb)
2143
2144
2145 Strain fitness (log2 ratio)
-2
-1
0
1 at 2142.660 kb on + strand, within PP_1898 at 2142.728 kb on + strand, within PP_1898 at 2142.728 kb on + strand, within PP_1898 at 2142.904 kb on + strand, within PP_1898 at 2143.041 kb on + strand, within PP_1898 at 2143.086 kb on + strand, within PP_1898 at 2143.316 kb on + strand, within PP_1899 at 2144.686 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. put PUT 10 MOI 1 remove 2,142,660 + PP_1898 0.16 +0.9 2,142,728 + PP_1898 0.26 +1.6 2,142,728 + PP_1898 0.26 +1.0 2,142,904 + PP_1898 0.52 -2.1 2,143,041 + PP_1898 0.73 -0.7 2,143,086 + PP_1898 0.79 +0.0 2,143,316 + PP_1899 0.22 +1.0 2,144,686 + -0.2
Or see this region's nucleotide sequence