Experiment: P. put PUT 10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1772 and PP_1773 are separated by 299 nucleotides PP_1773 and PP_1774 overlap by 32 nucleotides PP_1774 and PP_1775 are separated by 102 nucleotides PP_1775 and PP_1776 are separated by 327 nucleotides
PP_1772: PP_1772 - 30S ribosomal protein S1, at 1,979,331 to 1,981,061
_1772
PP_1773: PP_1773 - Integration host factor subunit beta, at 1,981,361 to 1,981,663
_1773
PP_1774: PP_1774 - conserved exported protein of unknown function, at 1,981,632 to 1,981,946
_1774
PP_1775: PP_1775 - Metallo-beta-lactamase family protein, at 1,982,049 to 1,983,482
_1775
PP_1776: PP_1776 - Mannose-6-phosphate isomerase/mannose-1-phosphate guanylyltransferase, at 1,983,810 to 1,985,252
_1776
Position (kb)
1982
1983
1984 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 1981.183 kb on - strand at 1981.221 kb on - strand at 1981.221 kb on - strand at 1981.224 kb on + strand at 1981.225 kb on - strand at 1981.225 kb on - strand at 1981.231 kb on + strand at 1981.271 kb on + strand at 1981.272 kb on - strand at 1981.360 kb on + strand at 1981.387 kb on + strand at 1981.387 kb on + strand at 1981.387 kb on + strand at 1981.388 kb on - strand at 1981.388 kb on - strand at 1981.726 kb on + strand, within PP_1774 at 1981.727 kb on - strand, within PP_1774 at 1981.727 kb on - strand, within PP_1774 at 1981.945 kb on - strand at 1981.945 kb on - strand at 1981.963 kb on + strand at 1981.963 kb on + strand at 1981.964 kb on - strand at 1981.964 kb on - strand at 1981.964 kb on - strand at 1981.964 kb on - strand at 1981.966 kb on + strand at 1981.967 kb on - strand at 1981.982 kb on + strand at 1981.982 kb on + strand at 1981.983 kb on - strand at 1981.983 kb on - strand at 1981.983 kb on - strand at 1981.983 kb on - strand at 1982.007 kb on + strand at 1982.008 kb on - strand at 1982.008 kb on - strand at 1982.008 kb on - strand at 1982.010 kb on + strand at 1982.011 kb on - strand at 1982.011 kb on - strand at 1982.052 kb on + strand at 1982.304 kb on - strand, within PP_1775 at 1982.359 kb on - strand, within PP_1775 at 1982.359 kb on - strand, within PP_1775 at 1982.401 kb on - strand, within PP_1775 at 1982.423 kb on + strand, within PP_1775 at 1982.424 kb on - strand, within PP_1775 at 1982.487 kb on - strand, within PP_1775 at 1982.526 kb on + strand, within PP_1775 at 1982.527 kb on - strand, within PP_1775 at 1982.527 kb on - strand, within PP_1775 at 1982.540 kb on + strand, within PP_1775 at 1982.569 kb on - strand, within PP_1775 at 1982.569 kb on - strand, within PP_1775 at 1982.664 kb on + strand, within PP_1775 at 1982.796 kb on + strand, within PP_1775 at 1983.057 kb on + strand, within PP_1775 at 1983.057 kb on + strand, within PP_1775 at 1983.132 kb on + strand, within PP_1775 at 1983.133 kb on - strand, within PP_1775 at 1983.212 kb on - strand, within PP_1775 at 1983.271 kb on - strand, within PP_1775 at 1983.271 kb on - strand, within PP_1775 at 1983.450 kb on - strand at 1983.470 kb on + strand at 1983.470 kb on + strand at 1983.470 kb on + strand at 1983.471 kb on - strand at 1983.471 kb on - strand at 1983.471 kb on - strand at 1983.471 kb on - strand at 1983.471 kb on - strand at 1983.481 kb on - strand at 1983.564 kb on + strand at 1983.565 kb on - strand at 1983.625 kb on + strand at 1983.625 kb on + strand at 1983.625 kb on + strand at 1983.625 kb on + strand at 1983.625 kb on + strand at 1983.626 kb on - strand at 1983.626 kb on - strand at 1983.626 kb on - strand at 1983.644 kb on + strand at 1983.644 kb on + strand at 1983.650 kb on - strand at 1983.710 kb on - strand at 1983.710 kb on - strand at 1983.713 kb on + strand at 1983.714 kb on - strand at 1983.748 kb on + strand at 1983.749 kb on - strand at 1983.751 kb on + strand at 1983.751 kb on + strand at 1983.751 kb on + strand at 1983.752 kb on - strand at 1983.752 kb on - strand at 1983.756 kb on + strand at 1983.756 kb on + strand at 1983.756 kb on + strand at 1983.756 kb on + strand at 1983.757 kb on - strand at 1983.757 kb on - strand at 1983.757 kb on - strand at 1983.865 kb on + strand at 1983.866 kb on - strand at 1984.053 kb on + strand, within PP_1776 at 1984.053 kb on + strand, within PP_1776 at 1984.053 kb on + strand, within PP_1776 at 1984.253 kb on + strand, within PP_1776 at 1984.254 kb on - strand, within PP_1776 at 1984.254 kb on - strand, within PP_1776 at 1984.264 kb on - strand, within PP_1776 at 1984.284 kb on + strand, within PP_1776 at 1984.284 kb on + strand, within PP_1776 at 1984.285 kb on - strand, within PP_1776 at 1984.324 kb on + strand, within PP_1776 at 1984.325 kb on - strand, within PP_1776 at 1984.325 kb on - strand, within PP_1776 at 1984.374 kb on + strand, within PP_1776 at 1984.375 kb on - strand, within PP_1776 at 1984.375 kb on - strand, within PP_1776 at 1984.375 kb on - strand, within PP_1776 at 1984.375 kb on - strand, within PP_1776 at 1984.375 kb on - strand, within PP_1776 at 1984.392 kb on + strand, within PP_1776 at 1984.393 kb on - strand, within PP_1776 at 1984.455 kb on + strand, within PP_1776 at 1984.455 kb on + strand, within PP_1776 at 1984.455 kb on + strand, within PP_1776 at 1984.456 kb on - strand at 1984.456 kb on - strand, within PP_1776
Per-strain Table
Position Strand Gene LocusTag Fraction P. put PUT 10 MOI 1 remove 1,981,183 - -3.3 1,981,221 - -3.5 1,981,221 - -4.3 1,981,224 + -3.7 1,981,225 - +0.6 1,981,225 - -4.8 1,981,231 + -3.7 1,981,271 + -3.3 1,981,272 - -2.3 1,981,360 + -3.2 1,981,387 + -3.0 1,981,387 + -3.9 1,981,387 + -3.7 1,981,388 - -4.1 1,981,388 - -5.0 1,981,726 + PP_1774 0.30 -5.1 1,981,727 - PP_1774 0.30 -3.0 1,981,727 - PP_1774 0.30 -3.7 1,981,945 - -2.5 1,981,945 - -4.3 1,981,963 + -3.8 1,981,963 + -3.4 1,981,964 - -4.2 1,981,964 - -4.1 1,981,964 - -3.7 1,981,964 - -2.8 1,981,966 + -4.9 1,981,967 - -4.2 1,981,982 + -5.4 1,981,982 + -4.4 1,981,983 - -4.0 1,981,983 - -1.4 1,981,983 - -3.0 1,981,983 - -3.7 1,982,007 + -3.0 1,982,008 - -4.3 1,982,008 - -3.8 1,982,008 - -2.8 1,982,010 + -4.8 1,982,011 - -4.6 1,982,011 - -4.6 1,982,052 + -5.1 1,982,304 - PP_1775 0.18 -0.0 1,982,359 - PP_1775 0.22 -4.2 1,982,359 - PP_1775 0.22 -3.9 1,982,401 - PP_1775 0.25 -3.3 1,982,423 + PP_1775 0.26 -3.7 1,982,424 - PP_1775 0.26 -4.2 1,982,487 - PP_1775 0.31 -2.8 1,982,526 + PP_1775 0.33 +0.7 1,982,527 - PP_1775 0.33 -4.4 1,982,527 - PP_1775 0.33 -4.2 1,982,540 + PP_1775 0.34 -4.4 1,982,569 - PP_1775 0.36 -5.1 1,982,569 - PP_1775 0.36 -0.4 1,982,664 + PP_1775 0.43 -2.8 1,982,796 + PP_1775 0.52 -4.3 1,983,057 + PP_1775 0.70 +1.8 1,983,057 + PP_1775 0.70 +2.0 1,983,132 + PP_1775 0.76 +2.2 1,983,133 - PP_1775 0.76 -3.7 1,983,212 - PP_1775 0.81 -1.7 1,983,271 - PP_1775 0.85 -1.6 1,983,271 - PP_1775 0.85 -3.5 1,983,450 - -3.9 1,983,470 + +1.8 1,983,470 + +2.1 1,983,470 + +1.2 1,983,471 - -0.9 1,983,471 - -3.3 1,983,471 - -4.1 1,983,471 - -4.9 1,983,471 - -3.2 1,983,481 - -2.2 1,983,564 + +3.6 1,983,565 - -3.9 1,983,625 + +2.8 1,983,625 + +1.9 1,983,625 + +1.8 1,983,625 + +2.5 1,983,625 + +2.3 1,983,626 - -2.8 1,983,626 - -3.0 1,983,626 - -2.7 1,983,644 + -4.5 1,983,644 + -3.1 1,983,650 - -3.5 1,983,710 - -3.0 1,983,710 - -3.0 1,983,713 + -3.4 1,983,714 - -3.6 1,983,748 + -4.5 1,983,749 - +0.0 1,983,751 + -1.1 1,983,751 + -4.5 1,983,751 + -4.2 1,983,752 - -4.1 1,983,752 - -3.4 1,983,756 + -3.1 1,983,756 + -2.1 1,983,756 + -3.8 1,983,756 + -3.4 1,983,757 - -2.1 1,983,757 - -2.5 1,983,757 - -3.6 1,983,865 + -3.6 1,983,866 - -2.3 1,984,053 + PP_1776 0.17 -3.7 1,984,053 + PP_1776 0.17 -4.2 1,984,053 + PP_1776 0.17 -2.2 1,984,253 + PP_1776 0.31 +1.6 1,984,254 - PP_1776 0.31 -2.0 1,984,254 - PP_1776 0.31 -2.9 1,984,264 - PP_1776 0.31 -2.7 1,984,284 + PP_1776 0.33 -3.2 1,984,284 + PP_1776 0.33 -3.2 1,984,285 - PP_1776 0.33 -2.5 1,984,324 + PP_1776 0.36 -1.9 1,984,325 - PP_1776 0.36 -3.7 1,984,325 - PP_1776 0.36 -3.6 1,984,374 + PP_1776 0.39 -4.4 1,984,375 - PP_1776 0.39 -3.6 1,984,375 - PP_1776 0.39 -4.0 1,984,375 - PP_1776 0.39 -3.1 1,984,375 - PP_1776 0.39 -1.8 1,984,375 - PP_1776 0.39 -2.5 1,984,392 + PP_1776 0.40 +0.0 1,984,393 - PP_1776 0.40 -1.8 1,984,455 + PP_1776 0.45 -3.4 1,984,455 + PP_1776 0.45 -3.7 1,984,455 + PP_1776 0.45 -1.4 1,984,456 - +1.6 1,984,456 - PP_1776 0.45 -4.3
Or see this region's nucleotide sequence