Experiment: P. put PUT 10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1772 and PP_1773 are separated by 299 nucleotides PP_1773 and PP_1774 overlap by 32 nucleotides PP_1774 and PP_1775 are separated by 102 nucleotides
PP_1772: PP_1772 - 30S ribosomal protein S1, at 1,979,331 to 1,981,061
_1772
PP_1773: PP_1773 - Integration host factor subunit beta, at 1,981,361 to 1,981,663
_1773
PP_1774: PP_1774 - conserved exported protein of unknown function, at 1,981,632 to 1,981,946
_1774
PP_1775: PP_1775 - Metallo-beta-lactamase family protein, at 1,982,049 to 1,983,482
_1775
Position (kb)
1981
1982 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1981.183 kb on - strand at 1981.221 kb on - strand at 1981.221 kb on - strand at 1981.224 kb on + strand at 1981.225 kb on - strand at 1981.225 kb on - strand at 1981.231 kb on + strand at 1981.271 kb on + strand at 1981.272 kb on - strand at 1981.360 kb on + strand at 1981.387 kb on + strand at 1981.387 kb on + strand at 1981.387 kb on + strand at 1981.388 kb on - strand at 1981.388 kb on - strand at 1981.726 kb on + strand, within PP_1774 at 1981.727 kb on - strand, within PP_1774 at 1981.727 kb on - strand, within PP_1774 at 1981.945 kb on - strand at 1981.945 kb on - strand at 1981.963 kb on + strand at 1981.963 kb on + strand at 1981.964 kb on - strand at 1981.964 kb on - strand at 1981.964 kb on - strand at 1981.964 kb on - strand at 1981.966 kb on + strand at 1981.967 kb on - strand at 1981.982 kb on + strand at 1981.982 kb on + strand at 1981.983 kb on - strand at 1981.983 kb on - strand at 1981.983 kb on - strand at 1981.983 kb on - strand at 1982.007 kb on + strand at 1982.008 kb on - strand at 1982.008 kb on - strand at 1982.008 kb on - strand at 1982.010 kb on + strand at 1982.011 kb on - strand at 1982.011 kb on - strand at 1982.052 kb on + strand at 1982.304 kb on - strand, within PP_1775 at 1982.359 kb on - strand, within PP_1775 at 1982.359 kb on - strand, within PP_1775 at 1982.401 kb on - strand, within PP_1775 at 1982.423 kb on + strand, within PP_1775 at 1982.424 kb on - strand, within PP_1775 at 1982.487 kb on - strand, within PP_1775 at 1982.526 kb on + strand, within PP_1775 at 1982.527 kb on - strand, within PP_1775 at 1982.527 kb on - strand, within PP_1775 at 1982.540 kb on + strand, within PP_1775 at 1982.569 kb on - strand, within PP_1775 at 1982.569 kb on - strand, within PP_1775 at 1982.664 kb on + strand, within PP_1775 at 1982.796 kb on + strand, within PP_1775
Per-strain Table
Position Strand Gene LocusTag Fraction P. put PUT 10 MOI 1 remove 1,981,183 - -3.3 1,981,221 - -3.5 1,981,221 - -4.3 1,981,224 + -3.7 1,981,225 - +0.6 1,981,225 - -4.8 1,981,231 + -3.7 1,981,271 + -3.3 1,981,272 - -2.3 1,981,360 + -3.2 1,981,387 + -3.0 1,981,387 + -3.9 1,981,387 + -3.7 1,981,388 - -4.1 1,981,388 - -5.0 1,981,726 + PP_1774 0.30 -5.1 1,981,727 - PP_1774 0.30 -3.0 1,981,727 - PP_1774 0.30 -3.7 1,981,945 - -2.5 1,981,945 - -4.3 1,981,963 + -3.8 1,981,963 + -3.4 1,981,964 - -4.2 1,981,964 - -4.1 1,981,964 - -3.7 1,981,964 - -2.8 1,981,966 + -4.9 1,981,967 - -4.2 1,981,982 + -5.4 1,981,982 + -4.4 1,981,983 - -4.0 1,981,983 - -1.4 1,981,983 - -3.0 1,981,983 - -3.7 1,982,007 + -3.0 1,982,008 - -4.3 1,982,008 - -3.8 1,982,008 - -2.8 1,982,010 + -4.8 1,982,011 - -4.6 1,982,011 - -4.6 1,982,052 + -5.1 1,982,304 - PP_1775 0.18 -0.0 1,982,359 - PP_1775 0.22 -4.2 1,982,359 - PP_1775 0.22 -3.9 1,982,401 - PP_1775 0.25 -3.3 1,982,423 + PP_1775 0.26 -3.7 1,982,424 - PP_1775 0.26 -4.2 1,982,487 - PP_1775 0.31 -2.8 1,982,526 + PP_1775 0.33 +0.7 1,982,527 - PP_1775 0.33 -4.4 1,982,527 - PP_1775 0.33 -4.2 1,982,540 + PP_1775 0.34 -4.4 1,982,569 - PP_1775 0.36 -5.1 1,982,569 - PP_1775 0.36 -0.4 1,982,664 + PP_1775 0.43 -2.8 1,982,796 + PP_1775 0.52 -4.3
Or see this region's nucleotide sequence