Experiment: P. put PUT 10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1604 and PP_1605 are separated by 1 nucleotides PP_1605 and PP_1606 are separated by 186 nucleotides PP_1606 and PP_1607 are separated by 106 nucleotides
PP_1604: PP_1604 - Lipid-A-disaccharide synthase, at 1,798,078 to 1,799,205
_1604
PP_1605: PP_1605 - Ribonuclease HII, at 1,799,207 to 1,799,830
_1605
PP_1606: PP_1606 - DNA polymerase III, alpha subunit, at 1,800,017 to 1,803,541
_1606
PP_1607: PP_1607 - acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha, at 1,803,648 to 1,804,595
_1607
Position (kb) 1800
1801
1802
1803
1804 Strain fitness (log2 ratio) -1
0
1 at 1799.236 kb on - strand at 1799.350 kb on + strand, within PP_1605 at 1799.512 kb on + strand, within PP_1605 at 1799.513 kb on - strand, within PP_1605 at 1799.749 kb on + strand, within PP_1605 at 1799.749 kb on + strand, within PP_1605 at 1799.965 kb on + strand at 1799.965 kb on + strand at 1799.966 kb on - strand at 1799.966 kb on - strand at 1799.969 kb on + strand at 1800.004 kb on + strand at 1803.555 kb on + strand at 1803.584 kb on + strand at 1803.589 kb on + strand at 1803.628 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. put PUT 10 MOI 1 remove 1,799,236 - +0.4 1,799,350 + PP_1605 0.23 -0.3 1,799,512 + PP_1605 0.49 +1.6 1,799,513 - PP_1605 0.49 -0.6 1,799,749 + PP_1605 0.87 +0.7 1,799,749 + PP_1605 0.87 -0.8 1,799,965 + +0.1 1,799,965 + -0.9 1,799,966 - -0.1 1,799,966 - -1.4 1,799,969 + +0.4 1,800,004 + -1.4 1,803,555 + +1.3 1,803,584 + +1.1 1,803,589 + +1.3 1,803,628 + +0.6
Or see this region's nucleotide sequence