Experiment: P. put PUT 10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1440 and PP_1441 overlap by 4 nucleotides PP_1441 and PP_1442 are separated by 89 nucleotides PP_1442 and PP_1443 are separated by 151 nucleotides
PP_1440: PP_1440 - tRNA (mo5U34)-methyltransferase, at 1,639,994 to 1,640,950
_1440
PP_1441: PP_1441 - tRNA (cmo5U34)-methyltransferase, at 1,640,947 to 1,641,690
_1441
PP_1442: PP_1442 - conserved protein of unknown function, at 1,641,780 to 1,642,172
_1442
PP_1443: PP_1443 - DNA-binding, ATP-dependent protease, at 1,642,324 to 1,644,741
_1443
Position (kb)
1641
1642
1643 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1640.960 kb on - strand at 1641.048 kb on + strand, within PP_1441 at 1641.049 kb on - strand, within PP_1441 at 1641.049 kb on - strand, within PP_1441 at 1641.108 kb on - strand, within PP_1441 at 1641.337 kb on + strand, within PP_1441 at 1641.338 kb on - strand, within PP_1441 at 1641.386 kb on + strand, within PP_1441 at 1641.420 kb on + strand, within PP_1441 at 1641.420 kb on + strand, within PP_1441 at 1641.506 kb on + strand at 1641.506 kb on + strand, within PP_1441 at 1641.732 kb on - strand at 1641.732 kb on - strand at 1641.732 kb on - strand at 1641.848 kb on - strand, within PP_1442 at 1641.848 kb on - strand, within PP_1442 at 1641.947 kb on - strand, within PP_1442 at 1641.973 kb on - strand, within PP_1442 at 1641.973 kb on - strand, within PP_1442 at 1642.034 kb on + strand, within PP_1442 at 1642.502 kb on - strand at 1642.637 kb on - strand, within PP_1443 at 1642.709 kb on + strand, within PP_1443 at 1642.709 kb on + strand, within PP_1443 at 1642.709 kb on + strand, within PP_1443 at 1642.710 kb on - strand, within PP_1443 at 1642.710 kb on - strand, within PP_1443 at 1642.710 kb on - strand, within PP_1443 at 1642.710 kb on - strand, within PP_1443 at 1642.710 kb on - strand, within PP_1443 at 1642.871 kb on - strand, within PP_1443 at 1642.871 kb on - strand, within PP_1443 at 1642.932 kb on + strand, within PP_1443 at 1642.933 kb on - strand, within PP_1443 at 1642.933 kb on - strand, within PP_1443 at 1642.933 kb on - strand, within PP_1443 at 1642.933 kb on - strand, within PP_1443 at 1642.933 kb on - strand, within PP_1443 at 1643.031 kb on - strand, within PP_1443 at 1643.031 kb on - strand, within PP_1443
Per-strain Table
Position Strand Gene LocusTag Fraction P. put PUT 10 MOI 1 remove 1,640,960 - -1.5 1,641,048 + PP_1441 0.14 -1.5 1,641,049 - PP_1441 0.14 -0.5 1,641,049 - PP_1441 0.14 -0.7 1,641,108 - PP_1441 0.22 -0.9 1,641,337 + PP_1441 0.52 -0.6 1,641,338 - PP_1441 0.53 -0.4 1,641,386 + PP_1441 0.59 -1.2 1,641,420 + PP_1441 0.64 +0.7 1,641,420 + PP_1441 0.64 +0.0 1,641,506 + +0.3 1,641,506 + PP_1441 0.75 -0.1 1,641,732 - -0.3 1,641,732 - -0.9 1,641,732 - -0.3 1,641,848 - PP_1442 0.17 -0.7 1,641,848 - PP_1442 0.17 -0.5 1,641,947 - PP_1442 0.42 +0.3 1,641,973 - PP_1442 0.49 -0.3 1,641,973 - PP_1442 0.49 -0.3 1,642,034 + PP_1442 0.65 -0.2 1,642,502 - -0.8 1,642,637 - PP_1443 0.13 -0.4 1,642,709 + PP_1443 0.16 -3.1 1,642,709 + PP_1443 0.16 +0.4 1,642,709 + PP_1443 0.16 +0.3 1,642,710 - PP_1443 0.16 +0.3 1,642,710 - PP_1443 0.16 +0.7 1,642,710 - PP_1443 0.16 -0.0 1,642,710 - PP_1443 0.16 -0.4 1,642,710 - PP_1443 0.16 +0.8 1,642,871 - PP_1443 0.23 -0.1 1,642,871 - PP_1443 0.23 +0.1 1,642,932 + PP_1443 0.25 -0.6 1,642,933 - PP_1443 0.25 -0.4 1,642,933 - PP_1443 0.25 +0.6 1,642,933 - PP_1443 0.25 -0.1 1,642,933 - PP_1443 0.25 -1.1 1,642,933 - PP_1443 0.25 -0.4 1,643,031 - PP_1443 0.29 -0.7 1,643,031 - PP_1443 0.29 +0.2
Or see this region's nucleotide sequence