Experiment: P. put PUT 10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0838 and PP_0839 are separated by 151 nucleotides PP_0839 and PP_0840 are separated by 3 nucleotides PP_0840 and PP_0841 are separated by 179 nucleotides
PP_0838: PP_0838 - inositol monophosphatase / glycerol-2-phosphatase, at 975,320 to 976,138
_0838
PP_0839: PP_0839 - tRNA Cm32/Um32 methyltransferase, at 976,290 to 977,045
_0839
PP_0840: PP_0840 - serine acetyltransferase, at 977,049 to 977,834
_0840
PP_0841: PP_0841 - DNA-binding transcriptional dual regulator 2Fe-2S cluster IscR, at 978,014 to 978,505
_0841
Position (kb) 976
977
978 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 976.230 kb on + strand at 976.248 kb on - strand at 976.302 kb on - strand at 976.322 kb on + strand at 976.508 kb on + strand, within PP_0839 at 976.854 kb on + strand, within PP_0839 at 976.941 kb on + strand, within PP_0839 at 976.942 kb on - strand, within PP_0839 at 976.942 kb on - strand, within PP_0839 at 977.072 kb on + strand at 977.073 kb on - strand at 977.083 kb on + strand at 977.084 kb on - strand at 977.298 kb on - strand, within PP_0840 at 977.522 kb on + strand, within PP_0840 at 977.588 kb on + strand, within PP_0840 at 977.622 kb on + strand, within PP_0840 at 977.714 kb on + strand, within PP_0840 at 977.715 kb on - strand, within PP_0840 at 977.715 kb on - strand, within PP_0840 at 977.715 kb on - strand, within PP_0840 at 977.715 kb on - strand, within PP_0840 at 977.748 kb on + strand, within PP_0840 at 977.748 kb on + strand, within PP_0840 at 977.749 kb on - strand, within PP_0840 at 977.749 kb on - strand, within PP_0840 at 977.749 kb on - strand, within PP_0840 at 977.749 kb on - strand, within PP_0840 at 978.009 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. put PUT 10 MOI 1 remove 976,230 + +0.0 976,248 - -0.6 976,302 - -2.0 976,322 + -3.0 976,508 + PP_0839 0.29 -0.5 976,854 + PP_0839 0.75 -1.0 976,941 + PP_0839 0.86 -1.2 976,942 - PP_0839 0.86 -0.1 976,942 - PP_0839 0.86 -0.5 977,072 + +0.4 977,073 - -0.2 977,083 + -0.2 977,084 - -1.6 977,298 - PP_0840 0.32 -1.0 977,522 + PP_0840 0.60 +0.9 977,588 + PP_0840 0.69 -1.2 977,622 + PP_0840 0.73 +0.4 977,714 + PP_0840 0.85 -0.9 977,715 - PP_0840 0.85 -0.4 977,715 - PP_0840 0.85 +0.7 977,715 - PP_0840 0.85 +0.6 977,715 - PP_0840 0.85 +0.5 977,748 + PP_0840 0.89 +0.3 977,748 + PP_0840 0.89 +1.1 977,749 - PP_0840 0.89 +1.5 977,749 - PP_0840 0.89 -0.0 977,749 - PP_0840 0.89 +0.8 977,749 - PP_0840 0.89 +0.8 978,009 + +1.6
Or see this region's nucleotide sequence