Experiment: P. put PUT 10 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0398 and PP_0399 are separated by 14 nucleotides PP_0399 and PP_0400 overlap by 1 nucleotides PP_0400 and PP_0401 are separated by 197 nucleotides PP_0401 and PP_0402 overlap by 4 nucleotides
PP_0398: PP_0398 - Thiosulfate sulfurtransferase GlpE, at 484,721 to 485,053
_0398
PP_0399: PP_0399 - Bis(5'-nucleosyl)-tetraphosphatase, symmetrical, at 485,068 to 485,934
_0399
PP_0400: PP_0400 - CO2+/MG2+ efflux protein, at 485,934 to 486,314
_0400
PP_0401: PP_0401 - Ribosomal RNA small subunit methyltransferase A, at 486,512 to 487,315
_0401
PP_0402: PP_0402 - 4-hydroxythreonine-4-phosphate dehydrogenase, at 487,312 to 488,301
_0402
Position (kb) 485
486
487 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 485.060 kb on + strand at 485.060 kb on + strand at 485.060 kb on + strand at 485.060 kb on + strand at 485.060 kb on + strand at 485.061 kb on - strand at 485.061 kb on - strand at 485.061 kb on - strand at 485.061 kb on - strand at 485.061 kb on - strand at 485.088 kb on + strand at 485.088 kb on + strand at 485.089 kb on - strand at 485.089 kb on - strand at 485.089 kb on - strand at 486.017 kb on - strand, within PP_0400 at 486.067 kb on + strand, within PP_0400 at 486.068 kb on - strand, within PP_0400 at 486.068 kb on - strand, within PP_0400 at 486.068 kb on - strand, within PP_0400 at 486.068 kb on - strand, within PP_0400 at 486.126 kb on + strand, within PP_0400 at 486.126 kb on + strand, within PP_0400 at 486.127 kb on - strand, within PP_0400 at 486.136 kb on - strand, within PP_0400 at 486.136 kb on - strand, within PP_0400 at 486.220 kb on + strand, within PP_0400 at 486.221 kb on - strand, within PP_0400 at 486.343 kb on - strand at 486.343 kb on - strand at 486.343 kb on - strand at 486.353 kb on - strand at 486.428 kb on + strand at 486.440 kb on + strand at 486.508 kb on + strand at 486.829 kb on - strand, within PP_0401 at 486.829 kb on - strand, within PP_0401 at 486.978 kb on + strand, within PP_0401 at 486.978 kb on + strand, within PP_0401 at 486.979 kb on - strand, within PP_0401 at 486.979 kb on - strand, within PP_0401 at 486.979 kb on - strand, within PP_0401 at 487.302 kb on + strand at 487.303 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. put PUT 10 MOI 1 remove 485,060 + -0.6 485,060 + -0.4 485,060 + -1.2 485,060 + -0.1 485,060 + +0.3 485,061 - -0.4 485,061 - -0.8 485,061 - +0.1 485,061 - -2.4 485,061 - -0.0 485,088 + -0.6 485,088 + +0.5 485,089 - -0.4 485,089 - +0.6 485,089 - -0.1 486,017 - PP_0400 0.22 -0.7 486,067 + PP_0400 0.35 -0.7 486,068 - PP_0400 0.35 -0.2 486,068 - PP_0400 0.35 -0.1 486,068 - PP_0400 0.35 -0.2 486,068 - PP_0400 0.35 -1.1 486,126 + PP_0400 0.50 -0.4 486,126 + PP_0400 0.50 -1.7 486,127 - PP_0400 0.51 +0.0 486,136 - PP_0400 0.53 -0.7 486,136 - PP_0400 0.53 -1.0 486,220 + PP_0400 0.75 -1.1 486,221 - PP_0400 0.75 -1.1 486,343 - -0.4 486,343 - -0.4 486,343 - +0.2 486,353 - -0.1 486,428 + +0.6 486,440 + -0.1 486,508 + -1.2 486,829 - PP_0401 0.39 -1.1 486,829 - PP_0401 0.39 -0.1 486,978 + PP_0401 0.58 -0.8 486,978 + PP_0401 0.58 +0.1 486,979 - PP_0401 0.58 +0.3 486,979 - PP_0401 0.58 -0.1 486,979 - PP_0401 0.58 -3.5 487,302 + -2.9 487,303 - +0.5
Or see this region's nucleotide sequence