Experiment: P. put South MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_t69 and PP_0723 are separated by 20 nucleotides PP_0723 and PP_0724 are separated by 4 nucleotides PP_0724 and PP_0725 are separated by 4 nucleotides
PP_t69: PP_t69 - tRNA-Gln, at 839,746 to 839,820
_t69
PP_0723: PP_0723 - 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, at 839,841 to 840,701
_0723
PP_0724: PP_0724 - Outer-membrane lipoprotein LolB, at 840,706 to 841,323
_0724
PP_0725: PP_0725 - conserved hypothetical TPR repeat protein, at 841,328 to 843,049
_0725
Position (kb) 840
841
842 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 839.722 kb on - strand at 839.722 kb on - strand at 839.724 kb on - strand at 839.724 kb on - strand at 839.736 kb on - strand at 839.738 kb on - strand at 839.877 kb on - strand at 839.877 kb on - strand at 841.376 kb on - strand at 841.413 kb on - strand at 841.513 kb on - strand, within PP_0725 at 841.631 kb on - strand, within PP_0725 at 841.633 kb on - strand, within PP_0725 at 841.660 kb on - strand, within PP_0725 at 841.660 kb on - strand, within PP_0725 at 841.774 kb on - strand, within PP_0725 at 841.774 kb on - strand, within PP_0725 at 841.774 kb on - strand, within PP_0725 at 841.881 kb on - strand, within PP_0725 at 841.894 kb on - strand, within PP_0725 at 842.098 kb on - strand, within PP_0725 at 842.098 kb on - strand, within PP_0725 at 842.278 kb on - strand, within PP_0725
Per-strain Table
Position Strand Gene LocusTag Fraction P. put South MOI 0.1 remove 839,722 - +0.9 839,722 - -1.6 839,724 - -0.4 839,724 - +1.5 839,736 - +1.8 839,738 - +0.7 839,877 - +2.1 839,877 - +2.1 841,376 - +0.1 841,413 - +0.7 841,513 - PP_0725 0.11 -2.5 841,631 - PP_0725 0.18 -0.4 841,633 - PP_0725 0.18 -1.7 841,660 - PP_0725 0.19 -1.3 841,660 - PP_0725 0.19 -1.7 841,774 - PP_0725 0.26 -1.3 841,774 - PP_0725 0.26 -2.8 841,774 - PP_0725 0.26 -2.0 841,881 - PP_0725 0.32 -1.4 841,894 - PP_0725 0.33 -0.6 842,098 - PP_0725 0.45 -2.6 842,098 - PP_0725 0.45 +1.1 842,278 - PP_0725 0.55 -1.6
Or see this region's nucleotide sequence