Experiment: P. put South MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0061 and PP_0062 are separated by 83 nucleotides PP_0062 and PP_0063 are separated by 41 nucleotides PP_0063 and PP_0064 are separated by 97 nucleotides PP_0064 and PP_0065 are separated by 11 nucleotides
PP_0061: PP_0061 - glycine-tRNA ligase alpha subunit, at 71,901 to 72,848
_0061
PP_0062: PP_0062 - 3-methyl-adenine DNA glycosylase I, at 72,932 to 73,483
_0062
PP_0063: PP_0063 - putative Lipid A biosynthesis lauroyl acyltransferase, at 73,525 to 74,412
_0063
PP_0064: PP_0064 - conserved protein of unknown function, at 74,510 to 74,824
_0064
PP_0065: PP_0065 - NAD-binding component of TrK potassium transporter, at 74,836 to 76,209
_0065
Position (kb) 73
74
75 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 72.893 kb on - strand at 72.893 kb on - strand at 72.893 kb on - strand at 72.895 kb on - strand at 72.917 kb on - strand at 72.932 kb on - strand at 72.932 kb on - strand at 72.969 kb on - strand at 73.073 kb on - strand, within PP_0062 at 73.073 kb on - strand, within PP_0062 at 73.112 kb on + strand, within PP_0062 at 73.112 kb on + strand, within PP_0062 at 73.113 kb on - strand, within PP_0062 at 73.113 kb on - strand, within PP_0062 at 73.113 kb on - strand, within PP_0062 at 73.113 kb on - strand, within PP_0062 at 73.128 kb on + strand, within PP_0062 at 73.406 kb on + strand, within PP_0062 at 73.406 kb on + strand, within PP_0062 at 73.407 kb on - strand, within PP_0062 at 73.489 kb on + strand at 73.489 kb on + strand at 73.490 kb on - strand at 73.490 kb on - strand at 73.490 kb on - strand at 73.512 kb on - strand at 73.662 kb on - strand, within PP_0063 at 73.746 kb on + strand at 73.747 kb on - strand, within PP_0063 at 73.858 kb on - strand, within PP_0063 at 73.915 kb on + strand, within PP_0063 at 73.915 kb on + strand, within PP_0063 at 73.915 kb on + strand, within PP_0063 at 73.915 kb on + strand, within PP_0063 at 73.916 kb on - strand, within PP_0063 at 74.082 kb on + strand, within PP_0063 at 74.195 kb on + strand, within PP_0063 at 74.196 kb on - strand, within PP_0063 at 74.275 kb on + strand, within PP_0063 at 74.275 kb on + strand, within PP_0063 at 74.275 kb on + strand, within PP_0063 at 74.276 kb on - strand, within PP_0063 at 74.276 kb on - strand, within PP_0063 at 74.276 kb on - strand, within PP_0063 at 74.276 kb on - strand, within PP_0063 at 74.276 kb on - strand, within PP_0063 at 74.329 kb on + strand at 74.330 kb on - strand at 74.330 kb on - strand at 74.330 kb on - strand at 74.330 kb on - strand at 74.341 kb on + strand at 74.353 kb on + strand at 74.353 kb on + strand at 74.354 kb on - strand at 74.354 kb on - strand at 74.354 kb on - strand at 74.410 kb on + strand at 74.410 kb on + strand at 74.411 kb on - strand at 74.411 kb on - strand at 74.411 kb on - strand at 74.411 kb on - strand at 74.411 kb on - strand at 74.411 kb on - strand at 74.431 kb on + strand at 74.431 kb on + strand at 74.431 kb on + strand at 74.431 kb on + strand at 74.431 kb on + strand at 74.497 kb on + strand at 74.497 kb on + strand at 74.498 kb on - strand at 74.646 kb on + strand, within PP_0064 at 74.647 kb on - strand, within PP_0064 at 74.647 kb on - strand, within PP_0064 at 74.647 kb on - strand, within PP_0064 at 74.654 kb on - strand, within PP_0064 at 74.679 kb on + strand, within PP_0064 at 74.680 kb on - strand, within PP_0064 at 74.696 kb on + strand, within PP_0064 at 74.985 kb on + strand, within PP_0065 at 75.196 kb on + strand, within PP_0065 at 75.196 kb on + strand, within PP_0065 at 75.209 kb on + strand, within PP_0065
Per-strain Table
Position Strand Gene LocusTag Fraction P. put South MOI 1 remove 72,893 - +0.4 72,893 - -0.2 72,893 - +1.7 72,895 - -0.9 72,917 - +0.2 72,932 - -0.2 72,932 - +0.1 72,969 - +2.0 73,073 - PP_0062 0.26 +0.5 73,073 - PP_0062 0.26 +0.7 73,112 + PP_0062 0.33 +0.2 73,112 + PP_0062 0.33 +0.7 73,113 - PP_0062 0.33 -0.7 73,113 - PP_0062 0.33 +0.7 73,113 - PP_0062 0.33 -0.4 73,113 - PP_0062 0.33 -1.1 73,128 + PP_0062 0.36 -0.2 73,406 + PP_0062 0.86 +0.1 73,406 + PP_0062 0.86 +0.3 73,407 - PP_0062 0.86 +1.1 73,489 + +0.2 73,489 + +0.1 73,490 - +3.0 73,490 - +2.9 73,490 - +3.5 73,512 - +0.2 73,662 - PP_0063 0.15 +1.4 73,746 + +2.8 73,747 - PP_0063 0.25 +3.6 73,858 - PP_0063 0.38 +2.4 73,915 + PP_0063 0.44 +2.5 73,915 + PP_0063 0.44 +2.2 73,915 + PP_0063 0.44 +3.9 73,915 + PP_0063 0.44 +2.4 73,916 - PP_0063 0.44 +3.8 74,082 + PP_0063 0.63 -1.9 74,195 + PP_0063 0.75 +2.3 74,196 - PP_0063 0.76 +3.2 74,275 + PP_0063 0.84 +3.2 74,275 + PP_0063 0.84 +3.0 74,275 + PP_0063 0.84 +2.5 74,276 - PP_0063 0.85 +3.2 74,276 - PP_0063 0.85 +2.9 74,276 - PP_0063 0.85 +0.5 74,276 - PP_0063 0.85 +2.4 74,276 - PP_0063 0.85 +1.4 74,329 + +2.3 74,330 - +3.0 74,330 - +4.3 74,330 - +2.5 74,330 - +2.7 74,341 + +2.8 74,353 + +3.0 74,353 + +2.4 74,354 - +4.5 74,354 - +2.1 74,354 - +2.4 74,410 + +0.8 74,410 + +0.3 74,411 - +0.1 74,411 - +0.6 74,411 - -0.7 74,411 - -0.3 74,411 - +1.2 74,411 - +0.2 74,431 + +1.9 74,431 + +0.4 74,431 + +1.3 74,431 + +0.7 74,431 + +1.5 74,497 + -1.3 74,497 + +0.3 74,498 - +0.9 74,646 + PP_0064 0.43 -0.9 74,647 - PP_0064 0.43 +0.3 74,647 - PP_0064 0.43 -0.2 74,647 - PP_0064 0.43 +0.6 74,654 - PP_0064 0.46 +1.4 74,679 + PP_0064 0.54 -0.6 74,680 - PP_0064 0.54 -0.6 74,696 + PP_0064 0.59 -0.1 74,985 + PP_0065 0.11 +0.1 75,196 + PP_0065 0.26 +2.2 75,196 + PP_0065 0.26 -0.2 75,209 + PP_0065 0.27 +0.3
Or see this region's nucleotide sequence