Experiment: P. put South MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4712 and PP_4713 are separated by 27 nucleotides PP_4713 and PP_4714 are separated by 59 nucleotides PP_4714 and PP_t49 are separated by 78 nucleotides PP_t49 and PP_t48 are separated by 100 nucleotides PP_t48 and PP_5706 are separated by 21 nucleotides
PP_4712: PP_4712 - Translation initiation factor IF-2, at 5,356,220 to 5,358,760
_4712
PP_4713: PP_4713 - transcription termination/antitermination L factor, at 5,358,788 to 5,360,269
_4713
PP_4714: PP_4714 - ribosome maturation factor, at 5,360,329 to 5,360,838
_4714
PP_t49: PP_t49 - tRNA-Met, at 5,360,917 to 5,360,993
_t49
PP_t48: PP_t48 - tRNA-Leu, at 5,361,094 to 5,361,179
_t48
PP_5706: PP_5706 - Sec translocation complex - SecG subunit, at 5,361,201 to 5,361,599
_5706
Position (kb) 5358
5359
5360
5361 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 5358.786 kb on - strand at 5360.957 kb on - strand, within PP_t49 at 5360.973 kb on - strand, within PP_t49 at 5361.186 kb on - strand at 5361.186 kb on - strand at 5361.189 kb on - strand at 5361.189 kb on - strand at 5361.229 kb on - strand at 5361.229 kb on - strand at 5361.229 kb on - strand at 5361.229 kb on - strand at 5361.240 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. put South MOI 1 remove 5,358,786 - -6.7 5,360,957 - PP_t49 0.52 -4.7 5,360,973 - PP_t49 0.73 -1.6 5,361,186 - -3.2 5,361,186 - -0.4 5,361,189 - -0.3 5,361,189 - -0.2 5,361,229 - +1.1 5,361,229 - -1.2 5,361,229 - +0.1 5,361,229 - +0.3 5,361,240 - -0.3
Or see this region's nucleotide sequence