Experiment: P. put South MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1964 and PP_1966 are separated by 446 nucleotides PP_1966 and PP_1967 are separated by 30 nucleotides
PP_1964: PP_1964 - putative dGMP, dTMP, dhydroxyCMP kinase, at 2,226,598 to 2,227,401
_1964
PP_1966: PP_1966 - DNA polymerase III subunit delta', at 2,227,848 to 2,228,834
_1966
PP_1967: PP_1967 - tRNA D-aminoacylase, at 2,228,865 to 2,229,647
_1967
Position (kb)
2227
2228
2229 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2227.443 kb on - strand at 2227.512 kb on + strand at 2227.526 kb on + strand at 2227.730 kb on + strand at 2227.730 kb on + strand at 2227.763 kb on + strand at 2227.818 kb on + strand at 2227.818 kb on + strand at 2228.860 kb on - strand at 2228.872 kb on + strand at 2228.872 kb on + strand at 2229.030 kb on + strand, within PP_1967 at 2229.030 kb on + strand, within PP_1967 at 2229.257 kb on + strand, within PP_1967 at 2229.257 kb on + strand, within PP_1967 at 2229.336 kb on - strand, within PP_1967 at 2229.378 kb on + strand, within PP_1967 at 2229.378 kb on + strand, within PP_1967 at 2229.381 kb on + strand, within PP_1967 at 2229.381 kb on + strand, within PP_1967 at 2229.381 kb on + strand, within PP_1967 at 2229.382 kb on - strand, within PP_1967 at 2229.531 kb on + strand, within PP_1967 at 2229.532 kb on - strand, within PP_1967 at 2229.552 kb on + strand, within PP_1967 at 2229.552 kb on + strand, within PP_1967 at 2229.553 kb on - strand, within PP_1967 at 2229.553 kb on - strand, within PP_1967 at 2229.579 kb on + strand at 2229.579 kb on + strand at 2229.579 kb on + strand at 2229.579 kb on + strand at 2229.754 kb on - strand at 2229.769 kb on - strand at 2229.779 kb on + strand at 2229.779 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction P. put South MOI 1 remove 2,227,443 - +4.2 2,227,512 + -1.7 2,227,526 + -0.1 2,227,730 + +0.5 2,227,730 + +0.7 2,227,763 + -0.2 2,227,818 + -0.6 2,227,818 + -3.8 2,228,860 - -0.9 2,228,872 + -0.4 2,228,872 + -0.3 2,229,030 + PP_1967 0.21 +0.1 2,229,030 + PP_1967 0.21 +0.8 2,229,257 + PP_1967 0.50 +0.1 2,229,257 + PP_1967 0.50 -0.0 2,229,336 - PP_1967 0.60 -0.7 2,229,378 + PP_1967 0.66 +1.7 2,229,378 + PP_1967 0.66 -0.5 2,229,381 + PP_1967 0.66 -0.2 2,229,381 + PP_1967 0.66 -0.5 2,229,381 + PP_1967 0.66 -0.1 2,229,382 - PP_1967 0.66 +0.5 2,229,531 + PP_1967 0.85 -0.7 2,229,532 - PP_1967 0.85 -0.6 2,229,552 + PP_1967 0.88 -0.5 2,229,552 + PP_1967 0.88 +0.7 2,229,553 - PP_1967 0.88 -0.9 2,229,553 - PP_1967 0.88 -3.4 2,229,579 + -0.2 2,229,579 + +0.2 2,229,579 + +0.3 2,229,579 + -0.6 2,229,754 - -0.5 2,229,769 - +1.0 2,229,779 + -0.8 2,229,779 + +0.8
Or see this region's nucleotide sequence