Strain Fitness in Pseudomonas putida KT2440 around PP_1770

Experiment: P. put South MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_1769 and PP_1770 are separated by 164 nucleotidesPP_1770 and PP_1771 overlap by 4 nucleotidesPP_1771 and PP_1772 are separated by 70 nucleotides PP_1769: PP_1769 - chorismate mutase/Prephenate dehydratase, at 1,975,069 to 1,976,172 _1769 PP_1770: PP_1770 - 3-phosphoshikimate 1-carboxyvinyltransferase, at 1,976,337 to 1,978,577 _1770 PP_1771: PP_1771 - Cytidylate kinase, at 1,978,574 to 1,979,260 _1771 PP_1772: PP_1772 - 30S ribosomal protein S1, at 1,979,331 to 1,981,061 _1772 Position (kb) 1976 1977 1978 1979Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 1976.311 kb on + strandat 1976.311 kb on + strandat 1976.341 kb on + strandat 1976.341 kb on + strandat 1977.243 kb on + strand, within PP_1770at 1977.243 kb on + strand, within PP_1770at 1977.243 kb on + strand, within PP_1770at 1977.254 kb on + strand, within PP_1770at 1979.254 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction P. put South MOI 1
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1,976,311 + -3.9
1,976,311 + -4.9
1,976,341 + -2.3
1,976,341 + +0.1
1,977,243 + PP_1770 0.40 -1.5
1,977,243 + PP_1770 0.40 -3.9
1,977,243 + PP_1770 0.40 -4.1
1,977,254 + PP_1770 0.41 -2.4
1,979,254 + -5.5

Or see this region's nucleotide sequence