Experiment: P. put South MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0066 and PP_0067 overlap by 4 nucleotides PP_0067 and PP_0068 are separated by 60 nucleotides PP_0068 and PP_0069 are separated by 206 nucleotides
PP_0066: PP_0066 - Ribosomal RNA small subunit methyltransferase B, at 76,233 to 77,543
_0066
PP_0067: PP_0067 - Methionyl-tRNA formyltransferase, at 77,540 to 78,472
_0067
PP_0068: PP_0068 - peptide deformylase, at 78,533 to 79,039
_0068
PP_0069: PP_0069 - putative Rossmann fold nucleotide-binding protein, at 79,246 to 80,343
_0069
Position (kb)
77
78
79 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 76.942 kb on + strand, within PP_0066 at 76.942 kb on + strand, within PP_0066 at 76.990 kb on + strand, within PP_0066 at 76.991 kb on - strand, within PP_0066 at 76.991 kb on - strand, within PP_0066 at 76.991 kb on - strand, within PP_0066 at 77.299 kb on + strand, within PP_0066 at 77.448 kb on + strand at 77.448 kb on + strand at 77.449 kb on - strand at 77.449 kb on - strand at 77.449 kb on - strand at 77.449 kb on - strand at 79.040 kb on - strand at 79.040 kb on - strand at 79.086 kb on - strand at 79.086 kb on - strand at 79.086 kb on - strand at 79.128 kb on - strand at 79.128 kb on - strand at 79.137 kb on - strand at 79.137 kb on - strand at 79.148 kb on - strand at 79.182 kb on - strand at 79.213 kb on + strand at 79.214 kb on - strand at 79.214 kb on - strand at 79.214 kb on - strand at 79.214 kb on - strand at 79.298 kb on + strand at 79.298 kb on + strand at 79.298 kb on + strand at 79.298 kb on + strand at 79.454 kb on + strand, within PP_0069 at 79.455 kb on - strand, within PP_0069
Per-strain Table
Position Strand Gene LocusTag Fraction P. put South MOI 1 remove 76,942 + PP_0066 0.54 -0.9 76,942 + PP_0066 0.54 -3.8 76,990 + PP_0066 0.58 -1.3 76,991 - PP_0066 0.58 -2.6 76,991 - PP_0066 0.58 -4.4 76,991 - PP_0066 0.58 -5.2 77,299 + PP_0066 0.81 -2.4 77,448 + -2.7 77,448 + -4.1 77,449 - -2.8 77,449 - -3.9 77,449 - -4.5 77,449 - -4.4 79,040 - -3.4 79,040 - -3.7 79,086 - -0.8 79,086 - -0.9 79,086 - -1.1 79,128 - -0.3 79,128 - -0.1 79,137 - -0.0 79,137 - -0.9 79,148 - +0.5 79,182 - -0.5 79,213 + -3.3 79,214 - -1.5 79,214 - +0.3 79,214 - -2.2 79,214 - -0.1 79,298 + -3.1 79,298 + +1.7 79,298 + -1.3 79,298 + -0.7 79,454 + PP_0069 0.19 -1.3 79,455 - PP_0069 0.19 -0.2
Or see this region's nucleotide sequence