Experiment: P. put South MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0010 and PP_0011 are separated by 40 nucleotides PP_0011 and PP_0012 are separated by 15 nucleotides
PP_0010: PP_0010 - chromosomal replication initiator protein, at 9,542 to 11,062
_0010
PP_0011: PP_0011 - DNA polymerase III subunit beta, at 11,103 to 12,206
_0011
PP_0012: PP_0012 - DNA replication and repair protein, at 12,222 to 13,325
_0012
Position (kb)
11
12
13 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 12.211 kb on + strand at 12.211 kb on + strand at 12.236 kb on + strand at 12.236 kb on + strand at 12.236 kb on + strand at 12.280 kb on + strand at 12.280 kb on + strand at 12.280 kb on + strand at 12.281 kb on - strand at 12.307 kb on - strand at 12.401 kb on + strand, within PP_0012 at 12.408 kb on + strand, within PP_0012 at 12.425 kb on + strand, within PP_0012 at 12.425 kb on + strand, within PP_0012 at 12.425 kb on + strand, within PP_0012 at 12.425 kb on + strand, within PP_0012 at 12.425 kb on + strand, within PP_0012 at 12.426 kb on - strand, within PP_0012 at 12.426 kb on - strand, within PP_0012 at 12.426 kb on - strand, within PP_0012 at 12.426 kb on - strand, within PP_0012 at 12.462 kb on - strand, within PP_0012 at 12.503 kb on + strand at 12.503 kb on + strand, within PP_0012 at 12.503 kb on + strand, within PP_0012 at 12.504 kb on - strand, within PP_0012 at 12.504 kb on - strand, within PP_0012 at 12.504 kb on - strand, within PP_0012 at 12.504 kb on - strand, within PP_0012 at 12.504 kb on - strand, within PP_0012 at 12.504 kb on - strand, within PP_0012 at 12.504 kb on - strand, within PP_0012 at 12.504 kb on - strand, within PP_0012 at 12.504 kb on - strand, within PP_0012 at 12.504 kb on - strand, within PP_0012 at 12.556 kb on + strand, within PP_0012 at 12.556 kb on + strand, within PP_0012 at 12.556 kb on + strand, within PP_0012 at 12.728 kb on + strand, within PP_0012 at 12.885 kb on + strand, within PP_0012 at 12.965 kb on + strand, within PP_0012 at 13.118 kb on + strand, within PP_0012 at 13.118 kb on + strand, within PP_0012 at 13.118 kb on + strand, within PP_0012 at 13.118 kb on + strand, within PP_0012 at 13.118 kb on + strand, within PP_0012 at 13.118 kb on + strand, within PP_0012 at 13.118 kb on + strand, within PP_0012 at 13.122 kb on + strand, within PP_0012 at 13.122 kb on + strand, within PP_0012 at 13.122 kb on + strand, within PP_0012 at 13.122 kb on + strand, within PP_0012
Per-strain Table
Position Strand Gene LocusTag Fraction P. put South MOI 1 remove 12,211 + +1.7 12,211 + -0.8 12,236 + -3.1 12,236 + -3.0 12,236 + -4.8 12,280 + -2.2 12,280 + +0.4 12,280 + +0.2 12,281 - -0.9 12,307 - +0.4 12,401 + PP_0012 0.16 -1.0 12,408 + PP_0012 0.17 +0.1 12,425 + PP_0012 0.18 -0.2 12,425 + PP_0012 0.18 -0.0 12,425 + PP_0012 0.18 +0.8 12,425 + PP_0012 0.18 -2.8 12,425 + PP_0012 0.18 +1.0 12,426 - PP_0012 0.18 +0.9 12,426 - PP_0012 0.18 +0.2 12,426 - PP_0012 0.18 +0.4 12,426 - PP_0012 0.18 +0.1 12,462 - PP_0012 0.22 -0.3 12,503 + +0.7 12,503 + PP_0012 0.25 -0.9 12,503 + PP_0012 0.25 +0.4 12,504 - PP_0012 0.26 -1.4 12,504 - PP_0012 0.26 -1.0 12,504 - PP_0012 0.26 +0.4 12,504 - PP_0012 0.26 +1.0 12,504 - PP_0012 0.26 -0.1 12,504 - PP_0012 0.26 +0.6 12,504 - PP_0012 0.26 +0.4 12,504 - PP_0012 0.26 +1.1 12,504 - PP_0012 0.26 +1.2 12,504 - PP_0012 0.26 -0.1 12,556 + PP_0012 0.30 +1.7 12,556 + PP_0012 0.30 +0.2 12,556 + PP_0012 0.30 +0.8 12,728 + PP_0012 0.46 -0.2 12,885 + PP_0012 0.60 -2.9 12,965 + PP_0012 0.67 -0.1 13,118 + PP_0012 0.81 +3.1 13,118 + PP_0012 0.81 -2.1 13,118 + PP_0012 0.81 -0.1 13,118 + PP_0012 0.81 -0.6 13,118 + PP_0012 0.81 -1.0 13,118 + PP_0012 0.81 +0.6 13,118 + PP_0012 0.81 +0.6 13,122 + PP_0012 0.82 +1.2 13,122 + PP_0012 0.82 +1.6 13,122 + PP_0012 0.82 -2.7 13,122 + PP_0012 0.82 -1.8
Or see this region's nucleotide sequence