Experiment: LB regrowth after 48hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yhbU and yhbV are separated by 8 nucleotides yhbV and yhbW are separated by 80 nucleotides
b3158: yhbU - predicted peptidase (collagenase-like) (NCBI), at 3,299,507 to 3,300,502
yhbU
b3159: yhbV - orf, hypothetical protein (VIMSS), at 3,300,511 to 3,301,389
yhbV
b3160: yhbW - predicted enzyme (NCBI), at 3,301,470 to 3,302,477
yhbW
Position (kb)
3300
3301
3302 Strain fitness (log2 ratio)
-2
-1
0
1 at 3299.515 kb on + strand at 3299.515 kb on + strand at 3299.550 kb on - strand at 3299.626 kb on + strand, within yhbU at 3299.785 kb on - strand, within yhbU at 3299.785 kb on - strand, within yhbU at 3299.809 kb on - strand, within yhbU at 3299.857 kb on + strand, within yhbU at 3299.860 kb on - strand, within yhbU at 3299.968 kb on + strand, within yhbU at 3299.968 kb on + strand, within yhbU at 3299.968 kb on + strand, within yhbU at 3299.981 kb on - strand, within yhbU at 3300.162 kb on + strand, within yhbU at 3300.216 kb on - strand, within yhbU at 3300.216 kb on - strand, within yhbU at 3300.225 kb on - strand, within yhbU at 3300.240 kb on - strand, within yhbU at 3300.349 kb on - strand, within yhbU at 3300.349 kb on - strand, within yhbU at 3300.388 kb on + strand, within yhbU at 3300.396 kb on - strand, within yhbU at 3300.396 kb on - strand, within yhbU at 3300.396 kb on - strand, within yhbU at 3300.396 kb on - strand, within yhbU at 3300.396 kb on - strand, within yhbU at 3300.451 kb on + strand at 3300.451 kb on + strand at 3300.451 kb on + strand at 3300.451 kb on + strand at 3300.476 kb on + strand at 3300.532 kb on + strand at 3300.540 kb on - strand at 3300.541 kb on - strand at 3300.543 kb on - strand at 3300.543 kb on - strand at 3300.545 kb on + strand at 3300.630 kb on - strand, within yhbV at 3300.632 kb on - strand, within yhbV at 3300.729 kb on + strand, within yhbV at 3300.742 kb on - strand, within yhbV at 3300.742 kb on - strand, within yhbV at 3300.742 kb on - strand, within yhbV at 3300.761 kb on + strand, within yhbV at 3300.861 kb on + strand, within yhbV at 3300.917 kb on - strand, within yhbV at 3300.922 kb on - strand, within yhbV at 3300.957 kb on + strand, within yhbV at 3300.993 kb on + strand, within yhbV at 3300.993 kb on + strand, within yhbV at 3301.040 kb on - strand, within yhbV at 3301.094 kb on - strand, within yhbV at 3301.094 kb on - strand, within yhbV at 3301.100 kb on + strand, within yhbV at 3301.100 kb on + strand, within yhbV at 3301.230 kb on + strand, within yhbV at 3301.230 kb on + strand, within yhbV at 3301.245 kb on + strand, within yhbV at 3301.245 kb on + strand, within yhbV at 3301.286 kb on - strand, within yhbV at 3301.286 kb on - strand, within yhbV at 3301.305 kb on - strand at 3301.305 kb on - strand at 3301.467 kb on + strand at 3301.666 kb on + strand, within yhbW at 3301.746 kb on - strand, within yhbW at 3301.751 kb on + strand, within yhbW at 3301.751 kb on + strand, within yhbW at 3301.768 kb on + strand, within yhbW at 3301.770 kb on - strand, within yhbW at 3301.774 kb on + strand, within yhbW at 3301.782 kb on - strand, within yhbW at 3301.794 kb on + strand, within yhbW at 3301.814 kb on - strand, within yhbW at 3301.816 kb on - strand, within yhbW at 3301.898 kb on - strand, within yhbW at 3301.986 kb on + strand, within yhbW at 3301.986 kb on + strand, within yhbW at 3302.135 kb on - strand, within yhbW at 3302.191 kb on - strand, within yhbW at 3302.206 kb on + strand, within yhbW at 3302.206 kb on + strand, within yhbW at 3302.281 kb on + strand, within yhbW at 3302.296 kb on - strand, within yhbW at 3302.318 kb on + strand, within yhbW at 3302.318 kb on - strand, within yhbW at 3302.349 kb on + strand, within yhbW
Per-strain Table
Position Strand Gene LocusTag Fraction LB regrowth after 48hrs remove 3,299,515 + -0.3 3,299,515 + -0.6 3,299,550 - -0.2 3,299,626 + yhbU b3158 0.12 -1.4 3,299,785 - yhbU b3158 0.28 -0.9 3,299,785 - yhbU b3158 0.28 -1.1 3,299,809 - yhbU b3158 0.30 -0.1 3,299,857 + yhbU b3158 0.35 -1.1 3,299,860 - yhbU b3158 0.35 -1.1 3,299,968 + yhbU b3158 0.46 -1.3 3,299,968 + yhbU b3158 0.46 -1.1 3,299,968 + yhbU b3158 0.46 -0.6 3,299,981 - yhbU b3158 0.48 -0.5 3,300,162 + yhbU b3158 0.66 -0.6 3,300,216 - yhbU b3158 0.71 -0.8 3,300,216 - yhbU b3158 0.71 -0.7 3,300,225 - yhbU b3158 0.72 -1.1 3,300,240 - yhbU b3158 0.74 -0.9 3,300,349 - yhbU b3158 0.85 -1.2 3,300,349 - yhbU b3158 0.85 -1.2 3,300,388 + yhbU b3158 0.88 -0.8 3,300,396 - yhbU b3158 0.89 -0.5 3,300,396 - yhbU b3158 0.89 -1.0 3,300,396 - yhbU b3158 0.89 -0.5 3,300,396 - yhbU b3158 0.89 -0.7 3,300,396 - yhbU b3158 0.89 -0.3 3,300,451 + -1.4 3,300,451 + +0.3 3,300,451 + -1.3 3,300,451 + -1.4 3,300,476 + -0.0 3,300,532 + -0.6 3,300,540 - -0.6 3,300,541 - -0.7 3,300,543 - -0.7 3,300,543 - -0.7 3,300,545 + -1.3 3,300,630 - yhbV b3159 0.14 -1.9 3,300,632 - yhbV b3159 0.14 -0.8 3,300,729 + yhbV b3159 0.25 -1.0 3,300,742 - yhbV b3159 0.26 -0.6 3,300,742 - yhbV b3159 0.26 -1.1 3,300,742 - yhbV b3159 0.26 -0.4 3,300,761 + yhbV b3159 0.28 -1.0 3,300,861 + yhbV b3159 0.40 -0.7 3,300,917 - yhbV b3159 0.46 -0.7 3,300,922 - yhbV b3159 0.47 -0.8 3,300,957 + yhbV b3159 0.51 -0.9 3,300,993 + yhbV b3159 0.55 -0.9 3,300,993 + yhbV b3159 0.55 -1.3 3,301,040 - yhbV b3159 0.60 -0.3 3,301,094 - yhbV b3159 0.66 -0.4 3,301,094 - yhbV b3159 0.66 -0.7 3,301,100 + yhbV b3159 0.67 -0.9 3,301,100 + yhbV b3159 0.67 -0.7 3,301,230 + yhbV b3159 0.82 -1.5 3,301,230 + yhbV b3159 0.82 -0.4 3,301,245 + yhbV b3159 0.84 -0.7 3,301,245 + yhbV b3159 0.84 -0.6 3,301,286 - yhbV b3159 0.88 -0.6 3,301,286 - yhbV b3159 0.88 -1.5 3,301,305 - -0.5 3,301,305 - -0.8 3,301,467 + +0.2 3,301,666 + yhbW b3160 0.19 +0.1 3,301,746 - yhbW b3160 0.27 -0.1 3,301,751 + yhbW b3160 0.28 -0.0 3,301,751 + yhbW b3160 0.28 +0.0 3,301,768 + yhbW b3160 0.30 +0.9 3,301,770 - yhbW b3160 0.30 +0.5 3,301,774 + yhbW b3160 0.30 +0.0 3,301,782 - yhbW b3160 0.31 -0.2 3,301,794 + yhbW b3160 0.32 +0.5 3,301,814 - yhbW b3160 0.34 +0.2 3,301,816 - yhbW b3160 0.34 +0.0 3,301,898 - yhbW b3160 0.42 +0.0 3,301,986 + yhbW b3160 0.51 +0.1 3,301,986 + yhbW b3160 0.51 +0.2 3,302,135 - yhbW b3160 0.66 +0.0 3,302,191 - yhbW b3160 0.72 +0.1 3,302,206 + yhbW b3160 0.73 +0.5 3,302,206 + yhbW b3160 0.73 -0.3 3,302,281 + yhbW b3160 0.80 +0.4 3,302,296 - yhbW b3160 0.82 +0.5 3,302,318 + yhbW b3160 0.84 +0.3 3,302,318 - yhbW b3160 0.84 -0.2 3,302,349 + yhbW b3160 0.87 +0.4
Or see this region's nucleotide sequence