Strain Fitness in Escherichia coli BW25113 around b4387

Experiment: LB + carbenicillin Time48hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntlplA and ytjB are separated by 27 nucleotidesytjB and serB are separated by 105 nucleotides b4386: lplA - lipoate-protein ligase A (NCBI), at 4,621,124 to 4,622,140 lplA b4387: ytjB - hypothetical protein (NCBI), at 4,622,168 to 4,622,812 ytjB b4388: serB - 3-phosphoserine phosphatase (NCBI), at 4,622,918 to 4,623,886 serB Position (kb) 4622 4623Strain fitness (log2 ratio) -1 0 1 2at 4621.232 kb on + strand, within lplAat 4621.240 kb on - strand, within lplAat 4621.240 kb on - strand, within lplAat 4621.240 kb on - strand, within lplAat 4621.287 kb on - strand, within lplAat 4621.298 kb on + strand, within lplAat 4621.298 kb on + strand, within lplAat 4621.301 kb on - strand, within lplAat 4621.350 kb on - strand, within lplAat 4621.350 kb on - strand, within lplAat 4621.457 kb on + strand, within lplAat 4621.457 kb on + strand, within lplAat 4621.491 kb on + strand, within lplAat 4621.501 kb on + strand, within lplAat 4621.549 kb on + strand, within lplAat 4621.549 kb on + strand, within lplAat 4621.592 kb on + strand, within lplAat 4621.674 kb on - strand, within lplAat 4621.674 kb on - strand, within lplAat 4621.869 kb on + strand, within lplAat 4621.884 kb on - strand, within lplAat 4621.946 kb on - strand, within lplAat 4621.946 kb on - strand, within lplAat 4621.952 kb on - strand, within lplAat 4621.957 kb on - strand, within lplAat 4621.970 kb on + strand, within lplAat 4621.970 kb on + strand, within lplAat 4621.976 kb on - strand, within lplAat 4622.011 kb on - strand, within lplAat 4622.011 kb on - strand, within lplAat 4622.126 kb on + strandat 4622.281 kb on - strand, within ytjBat 4622.281 kb on - strand, within ytjBat 4622.284 kb on - strand, within ytjBat 4622.284 kb on - strand, within ytjBat 4622.289 kb on - strand, within ytjBat 4622.305 kb on + strand, within ytjBat 4622.531 kb on + strand, within ytjBat 4622.534 kb on + strand, within ytjBat 4622.539 kb on - strand, within ytjBat 4622.539 kb on - strand, within ytjBat 4622.637 kb on - strand, within ytjBat 4622.653 kb on + strand, within ytjBat 4622.705 kb on - strand, within ytjBat 4622.816 kb on - strandat 4622.846 kb on + strandat 4622.901 kb on + strandat 4622.901 kb on + strandat 4622.925 kb on + strandat 4622.925 kb on + strandat 4622.925 kb on + strandat 4622.944 kb on + strandat 4622.952 kb on - strandat 4622.952 kb on - strandat 4622.977 kb on - strandat 4623.023 kb on + strand, within serBat 4623.023 kb on - strand, within serBat 4623.038 kb on + strand, within serBat 4623.038 kb on + strand, within serBat 4623.048 kb on + strand, within serBat 4623.048 kb on + strand, within serBat 4623.056 kb on - strand, within serBat 4623.109 kb on - strand, within serBat 4623.126 kb on - strand, within serBat 4623.126 kb on - strand, within serBat 4623.202 kb on + strand, within serBat 4623.296 kb on + strand, within serBat 4623.352 kb on - strand, within serBat 4623.352 kb on - strand, within serBat 4623.551 kb on + strand, within serBat 4623.691 kb on + strand, within serBat 4623.691 kb on + strand, within serBat 4623.746 kb on - strand, within serB

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + carbenicillin Time48hr
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4,621,232 + lplA b4386 0.11 +0.4
4,621,240 - lplA b4386 0.11 -0.2
4,621,240 - lplA b4386 0.11 +0.4
4,621,240 - lplA b4386 0.11 +0.2
4,621,287 - lplA b4386 0.16 -0.1
4,621,298 + lplA b4386 0.17 -0.4
4,621,298 + lplA b4386 0.17 +0.2
4,621,301 - lplA b4386 0.17 +0.3
4,621,350 - lplA b4386 0.22 +0.1
4,621,350 - lplA b4386 0.22 +0.3
4,621,457 + lplA b4386 0.33 +1.0
4,621,457 + lplA b4386 0.33 +0.3
4,621,491 + lplA b4386 0.36 +0.2
4,621,501 + lplA b4386 0.37 +0.2
4,621,549 + lplA b4386 0.42 +0.7
4,621,549 + lplA b4386 0.42 +0.2
4,621,592 + lplA b4386 0.46 +0.3
4,621,674 - lplA b4386 0.54 +0.5
4,621,674 - lplA b4386 0.54 +0.8
4,621,869 + lplA b4386 0.73 +0.5
4,621,884 - lplA b4386 0.75 +0.3
4,621,946 - lplA b4386 0.81 +0.3
4,621,946 - lplA b4386 0.81 +0.5
4,621,952 - lplA b4386 0.81 +0.6
4,621,957 - lplA b4386 0.82 +0.3
4,621,970 + lplA b4386 0.83 +0.4
4,621,970 + lplA b4386 0.83 +0.2
4,621,976 - lplA b4386 0.84 -0.1
4,622,011 - lplA b4386 0.87 -0.2
4,622,011 - lplA b4386 0.87 +0.4
4,622,126 + +0.6
4,622,281 - ytjB b4387 0.18 -0.2
4,622,281 - ytjB b4387 0.18 +0.4
4,622,284 - ytjB b4387 0.18 +0.4
4,622,284 - ytjB b4387 0.18 +2.0
4,622,289 - ytjB b4387 0.19 +0.2
4,622,305 + ytjB b4387 0.21 +0.2
4,622,531 + ytjB b4387 0.56 +0.2
4,622,534 + ytjB b4387 0.57 +0.5
4,622,539 - ytjB b4387 0.58 +0.5
4,622,539 - ytjB b4387 0.58 +0.6
4,622,637 - ytjB b4387 0.73 +1.2
4,622,653 + ytjB b4387 0.75 +0.0
4,622,705 - ytjB b4387 0.83 +0.4
4,622,816 - +0.6
4,622,846 + +0.2
4,622,901 + -0.2
4,622,901 + +0.4
4,622,925 + -0.3
4,622,925 + +0.9
4,622,925 + +0.4
4,622,944 + +0.6
4,622,952 - +0.2
4,622,952 - +0.9
4,622,977 - -0.3
4,623,023 + serB b4388 0.11 -0.2
4,623,023 - serB b4388 0.11 +0.8
4,623,038 + serB b4388 0.12 +0.6
4,623,038 + serB b4388 0.12 +0.6
4,623,048 + serB b4388 0.13 -0.6
4,623,048 + serB b4388 0.13 -0.2
4,623,056 - serB b4388 0.14 +0.4
4,623,109 - serB b4388 0.20 +0.3
4,623,126 - serB b4388 0.21 -0.0
4,623,126 - serB b4388 0.21 -0.1
4,623,202 + serB b4388 0.29 +0.2
4,623,296 + serB b4388 0.39 +0.2
4,623,352 - serB b4388 0.45 -0.3
4,623,352 - serB b4388 0.45 +0.9
4,623,551 + serB b4388 0.65 +0.2
4,623,691 + serB b4388 0.80 +0.5
4,623,691 + serB b4388 0.80 +0.2
4,623,746 - serB b4388 0.85 +0.3

Or see this region's nucleotide sequence