Strain Fitness in Escherichia coli BW25113 around b2831

Experiment: LB + carbenicillin Time48hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnudH and mutH are separated by 684 nucleotidesmutH and ygdQ are separated by 68 nucleotidesygdQ and ygdR are separated by 137 nucleotides b2830: nudH - dinucleoside polyphosphate hydrolase (NCBI), at 2,966,469 to 2,966,999 nudH b2831: mutH - DNA mismatch repair protein (NCBI), at 2,967,684 to 2,968,373 mutH b2832: ygdQ - predicted inner membrane protein (NCBI), at 2,968,442 to 2,969,155 ygdQ b2833: ygdR - hypothetical protein (NCBI), at 2,969,293 to 2,969,511 ygdR Position (kb) 2967 2968 2969Strain fitness (log2 ratio) -1 0 1at 2966.722 kb on + strand, within nudHat 2967.071 kb on + strandat 2967.078 kb on + strandat 2967.078 kb on + strandat 2967.640 kb on - strandat 2967.694 kb on - strandat 2967.728 kb on + strandat 2967.768 kb on - strand, within mutHat 2967.768 kb on - strand, within mutHat 2967.994 kb on - strand, within mutHat 2968.044 kb on + strand, within mutHat 2968.088 kb on + strand, within mutHat 2968.088 kb on + strand, within mutHat 2968.096 kb on - strand, within mutHat 2968.174 kb on + strand, within mutHat 2968.179 kb on - strand, within mutHat 2968.183 kb on - strand, within mutHat 2968.499 kb on + strandat 2968.509 kb on - strandat 2968.509 kb on - strandat 2968.614 kb on - strand, within ygdQat 2968.614 kb on - strand, within ygdQat 2968.620 kb on - strand, within ygdQat 2968.620 kb on - strand, within ygdQat 2968.648 kb on + strand, within ygdQat 2968.648 kb on + strand, within ygdQat 2968.674 kb on + strand, within ygdQat 2968.786 kb on - strand, within ygdQat 2968.829 kb on + strand, within ygdQat 2968.856 kb on - strand, within ygdQat 2968.880 kb on + strand, within ygdQat 2968.942 kb on + strand, within ygdQat 2968.942 kb on + strand, within ygdQat 2968.955 kb on - strand, within ygdQat 2969.039 kb on - strand, within ygdQat 2969.039 kb on - strand, within ygdQat 2969.108 kb on - strandat 2969.108 kb on - strandat 2969.160 kb on - strandat 2969.160 kb on - strandat 2969.164 kb on + strandat 2969.172 kb on - strandat 2969.172 kb on - strandat 2969.193 kb on + strandat 2969.193 kb on - strandat 2969.202 kb on - strandat 2969.231 kb on + strandat 2969.242 kb on - strandat 2969.263 kb on + strandat 2969.263 kb on + strandat 2969.280 kb on - strandat 2969.281 kb on - strandat 2969.286 kb on - strandat 2969.327 kb on + strand, within ygdR

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + carbenicillin Time48hr
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2,966,722 + nudH b2830 0.48 +1.7
2,967,071 + +0.3
2,967,078 + -0.2
2,967,078 + +0.5
2,967,640 - -0.3
2,967,694 - +0.7
2,967,728 + +0.4
2,967,768 - mutH b2831 0.12 -0.7
2,967,768 - mutH b2831 0.12 +0.5
2,967,994 - mutH b2831 0.45 -0.1
2,968,044 + mutH b2831 0.52 -0.0
2,968,088 + mutH b2831 0.59 +0.0
2,968,088 + mutH b2831 0.59 -0.2
2,968,096 - mutH b2831 0.60 -0.0
2,968,174 + mutH b2831 0.71 +0.3
2,968,179 - mutH b2831 0.72 -0.2
2,968,183 - mutH b2831 0.72 -0.5
2,968,499 + +0.1
2,968,509 - +0.2
2,968,509 - -0.3
2,968,614 - ygdQ b2832 0.24 +0.3
2,968,614 - ygdQ b2832 0.24 -0.0
2,968,620 - ygdQ b2832 0.25 -0.7
2,968,620 - ygdQ b2832 0.25 +0.2
2,968,648 + ygdQ b2832 0.29 +0.3
2,968,648 + ygdQ b2832 0.29 +0.3
2,968,674 + ygdQ b2832 0.32 +0.1
2,968,786 - ygdQ b2832 0.48 +0.5
2,968,829 + ygdQ b2832 0.54 +0.2
2,968,856 - ygdQ b2832 0.58 +0.1
2,968,880 + ygdQ b2832 0.61 +0.3
2,968,942 + ygdQ b2832 0.70 -0.6
2,968,942 + ygdQ b2832 0.70 +1.1
2,968,955 - ygdQ b2832 0.72 +0.1
2,969,039 - ygdQ b2832 0.84 -0.9
2,969,039 - ygdQ b2832 0.84 +0.6
2,969,108 - +0.4
2,969,108 - -0.3
2,969,160 - +0.1
2,969,160 - +0.2
2,969,164 + +0.0
2,969,172 - -0.2
2,969,172 - -0.1
2,969,193 + +0.0
2,969,193 - +0.2
2,969,202 - -0.3
2,969,231 + -0.1
2,969,242 - -0.1
2,969,263 + +1.3
2,969,263 + +1.0
2,969,280 - +0.0
2,969,281 - +0.3
2,969,286 - -0.4
2,969,327 + ygdR b2833 0.16 -0.0

Or see this region's nucleotide sequence