Experiment: LB + carbenicillin Time48hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dxs and ispA are separated by 24 nucleotides ispA and xseB overlap by 1 nucleotides xseB and thiI are separated by 205 nucleotides
b0420: dxs - 1-deoxy-D-xylulose-5-phosphate synthase (NCBI), at 437,539 to 439,401
dxs
b0421: ispA - geranyltranstransferase (NCBI), at 439,426 to 440,325
ispA
b0422: xseB - exodeoxyribonuclease VII small subunit (NCBI), at 440,325 to 440,567
xseB
b0423: thiI - thiamine biosynthesis protein ThiI (NCBI), at 440,773 to 442,221
thiI
Position (kb) 440
441 Strain fitness (log2 ratio) -1
0
1
2 at 439.386 kb on - strand at 439.407 kb on - strand at 440.335 kb on - strand at 440.546 kb on - strand at 440.581 kb on - strand at 440.581 kb on - strand at 440.609 kb on + strand at 440.650 kb on + strand at 440.650 kb on + strand at 440.650 kb on - strand at 440.716 kb on - strand at 440.750 kb on - strand at 440.882 kb on + strand at 440.882 kb on + strand at 440.882 kb on + strand at 440.890 kb on - strand at 440.890 kb on - strand at 440.968 kb on + strand, within thiI at 440.968 kb on + strand, within thiI at 440.976 kb on - strand, within thiI at 440.976 kb on - strand, within thiI at 440.976 kb on - strand, within thiI at 441.031 kb on - strand, within thiI at 441.031 kb on - strand, within thiI at 441.040 kb on - strand, within thiI at 441.066 kb on - strand, within thiI at 441.100 kb on + strand, within thiI at 441.430 kb on - strand, within thiI at 441.471 kb on + strand, within thiI
Per-strain Table
Position Strand Gene LocusTag Fraction LB + carbenicillin Time48hr remove 439,386 - +0.4 439,407 - -0.2 440,335 - -0.1 440,546 - -0.7 440,581 - +0.3 440,581 - -0.5 440,609 + +0.0 440,650 + -0.5 440,650 + +0.1 440,650 - +0.9 440,716 - +2.1 440,750 - +0.3 440,882 + -0.1 440,882 + -0.0 440,882 + -0.0 440,890 - +1.8 440,890 - +1.5 440,968 + thiI b0423 0.13 +0.5 440,968 + thiI b0423 0.13 -0.1 440,976 - thiI b0423 0.14 +0.1 440,976 - thiI b0423 0.14 -0.9 440,976 - thiI b0423 0.14 -0.3 441,031 - thiI b0423 0.18 +0.8 441,031 - thiI b0423 0.18 +0.4 441,040 - thiI b0423 0.18 +0.1 441,066 - thiI b0423 0.20 +0.0 441,100 + thiI b0423 0.23 +0.2 441,430 - thiI b0423 0.45 +0.0 441,471 + thiI b0423 0.48 -0.3
Or see this region's nucleotide sequence