Experiment: LB + carbenicillin Time48hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lfhA and mbhA are separated by 1 nucleotides mbhA and dinB are separated by 70 nucleotides dinB and yafN are separated by 51 nucleotides yafN and yafO are separated by 2 nucleotides yafO and yafP are separated by 9 nucleotides
b0229: lfhA - flagellar biosynthesis (VIMSS), at 248,358 to 250,070
lfhA
b0230: mbhA - putative motility protein (VIMSS), at 250,072 to 250,827
mbhA
b0231: dinB - DNA polymerase IV (NCBI), at 250,898 to 251,953
dinB
b0232: yafN - predicted antitoxin of the YafO-YafN toxin-antitoxin system (NCBI), at 252,005 to 252,298
yafN
b0233: yafO - predicted toxin of the YafO-YafN toxin-antitoxin system (NCBI), at 252,301 to 252,699
yafO
b0234: yafP - predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI), at 252,709 to 253,161
yafP
Position (kb) 250
251
252 Strain fitness (log2 ratio) -2
-1
0
1
2 at 250.046 kb on + strand at 250.049 kb on + strand at 250.049 kb on + strand at 250.295 kb on - strand, within mbhA at 250.295 kb on - strand, within mbhA at 250.355 kb on + strand, within mbhA at 250.374 kb on + strand, within mbhA at 250.389 kb on + strand, within mbhA at 250.426 kb on + strand, within mbhA at 250.442 kb on - strand, within mbhA at 250.473 kb on - strand, within mbhA at 250.501 kb on + strand, within mbhA at 250.559 kb on - strand, within mbhA at 250.594 kb on - strand, within mbhA at 250.697 kb on + strand, within mbhA at 250.718 kb on - strand, within mbhA at 250.718 kb on - strand, within mbhA at 250.729 kb on + strand, within mbhA at 250.959 kb on + strand at 250.988 kb on - strand at 251.129 kb on - strand at 251.129 kb on - strand, within dinB at 251.214 kb on + strand, within dinB at 251.240 kb on + strand, within dinB at 251.240 kb on + strand, within dinB at 251.240 kb on - strand, within dinB at 251.240 kb on - strand, within dinB at 251.255 kb on - strand, within dinB at 251.499 kb on - strand, within dinB at 251.499 kb on - strand, within dinB at 251.579 kb on - strand, within dinB at 251.662 kb on - strand, within dinB at 251.669 kb on + strand, within dinB at 251.803 kb on + strand, within dinB at 251.803 kb on + strand, within dinB at 251.818 kb on + strand, within dinB at 251.880 kb on + strand at 251.901 kb on + strand at 251.909 kb on - strand at 251.924 kb on + strand at 251.965 kb on - strand at 252.003 kb on - strand at 252.137 kb on - strand, within yafN at 252.155 kb on - strand, within yafN at 252.288 kb on + strand at 252.331 kb on + strand at 252.331 kb on + strand at 252.341 kb on - strand, within yafO at 252.346 kb on + strand, within yafO at 252.368 kb on - strand, within yafO at 252.368 kb on - strand, within yafO at 252.380 kb on + strand, within yafO at 252.381 kb on + strand, within yafO at 252.419 kb on + strand, within yafO at 252.427 kb on + strand, within yafO at 252.427 kb on + strand, within yafO at 252.440 kb on + strand, within yafO at 252.440 kb on + strand, within yafO at 252.440 kb on + strand, within yafO at 252.440 kb on + strand, within yafO at 252.440 kb on + strand, within yafO at 252.443 kb on - strand, within yafO at 252.475 kb on + strand, within yafO at 252.522 kb on - strand, within yafO at 252.522 kb on - strand, within yafO at 252.534 kb on - strand, within yafO at 252.538 kb on + strand, within yafO at 252.538 kb on + strand, within yafO at 252.563 kb on + strand, within yafO at 252.566 kb on - strand, within yafO at 252.574 kb on - strand, within yafO at 252.574 kb on - strand, within yafO at 252.592 kb on - strand, within yafO at 252.594 kb on - strand, within yafO at 252.597 kb on + strand, within yafO at 252.610 kb on - strand, within yafO at 252.614 kb on + strand, within yafO at 252.622 kb on - strand, within yafO at 252.628 kb on + strand, within yafO at 252.671 kb on + strand at 252.671 kb on + strand at 252.739 kb on - strand at 252.739 kb on - strand at 252.779 kb on - strand, within yafP at 252.781 kb on + strand, within yafP at 252.848 kb on + strand, within yafP at 252.849 kb on + strand, within yafP at 252.879 kb on + strand, within yafP at 252.896 kb on + strand, within yafP at 252.936 kb on + strand, within yafP
Per-strain Table
Position Strand Gene LocusTag Fraction LB + carbenicillin Time48hr remove 250,046 + +0.7 250,049 + +0.3 250,049 + -0.1 250,295 - mbhA b0230 0.29 +0.1 250,295 - mbhA b0230 0.29 -0.1 250,355 + mbhA b0230 0.37 +0.3 250,374 + mbhA b0230 0.40 +0.4 250,389 + mbhA b0230 0.42 +0.1 250,426 + mbhA b0230 0.47 +0.3 250,442 - mbhA b0230 0.49 +0.1 250,473 - mbhA b0230 0.53 +0.5 250,501 + mbhA b0230 0.57 +0.0 250,559 - mbhA b0230 0.64 +0.2 250,594 - mbhA b0230 0.69 +0.8 250,697 + mbhA b0230 0.83 -0.0 250,718 - mbhA b0230 0.85 +0.2 250,718 - mbhA b0230 0.85 +0.1 250,729 + mbhA b0230 0.87 +0.0 250,959 + -0.0 250,988 - -0.4 251,129 - +2.1 251,129 - dinB b0231 0.22 -0.2 251,214 + dinB b0231 0.30 -0.1 251,240 + dinB b0231 0.32 -0.1 251,240 + dinB b0231 0.32 -0.5 251,240 - dinB b0231 0.32 -0.0 251,240 - dinB b0231 0.32 +0.2 251,255 - dinB b0231 0.34 +0.0 251,499 - dinB b0231 0.57 +0.3 251,499 - dinB b0231 0.57 +0.2 251,579 - dinB b0231 0.64 -0.4 251,662 - dinB b0231 0.72 -0.1 251,669 + dinB b0231 0.73 +0.1 251,803 + dinB b0231 0.86 +0.2 251,803 + dinB b0231 0.86 +0.3 251,818 + dinB b0231 0.87 +0.4 251,880 + +0.1 251,901 + +0.2 251,909 - +0.1 251,924 + +0.7 251,965 - +0.6 252,003 - +0.2 252,137 - yafN b0232 0.45 -0.4 252,155 - yafN b0232 0.51 -0.4 252,288 + +0.1 252,331 + -0.1 252,331 + -0.2 252,341 - yafO b0233 0.10 +0.6 252,346 + yafO b0233 0.11 +0.9 252,368 - yafO b0233 0.17 +0.6 252,368 - yafO b0233 0.17 +0.6 252,380 + yafO b0233 0.20 -0.5 252,381 + yafO b0233 0.20 +0.2 252,419 + yafO b0233 0.30 +0.6 252,427 + yafO b0233 0.32 +0.6 252,427 + yafO b0233 0.32 +1.6 252,440 + yafO b0233 0.35 +1.4 252,440 + yafO b0233 0.35 +0.1 252,440 + yafO b0233 0.35 +0.8 252,440 + yafO b0233 0.35 +0.2 252,440 + yafO b0233 0.35 -0.4 252,443 - yafO b0233 0.36 +0.6 252,475 + yafO b0233 0.44 -0.4 252,522 - yafO b0233 0.55 -0.2 252,522 - yafO b0233 0.55 -0.4 252,534 - yafO b0233 0.58 -1.8 252,538 + yafO b0233 0.59 -0.0 252,538 + yafO b0233 0.59 -0.0 252,563 + yafO b0233 0.66 +0.1 252,566 - yafO b0233 0.66 +0.6 252,574 - yafO b0233 0.68 -0.3 252,574 - yafO b0233 0.68 +0.8 252,592 - yafO b0233 0.73 +0.6 252,594 - yafO b0233 0.73 +0.6 252,597 + yafO b0233 0.74 +0.7 252,610 - yafO b0233 0.77 +0.6 252,614 + yafO b0233 0.78 +0.6 252,622 - yafO b0233 0.80 +0.6 252,628 + yafO b0233 0.82 +0.7 252,671 + +0.0 252,671 + -0.3 252,739 - +0.6 252,739 - +0.6 252,779 - yafP b0234 0.15 +0.6 252,781 + yafP b0234 0.16 +0.6 252,848 + yafP b0234 0.31 -1.4 252,849 + yafP b0234 0.31 +0.6 252,879 + yafP b0234 0.38 +0.6 252,896 + yafP b0234 0.41 +0.6 252,936 + yafP b0234 0.50 +0.9
Or see this region's nucleotide sequence