Experiment: LB Time24hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gatC and gatB are separated by 3 nucleotides gatB and gatA are separated by 30 nucleotides gatA and gatZ are separated by 9 nucleotides gatZ and gatY are separated by 28 nucleotides
b2092: gatC - galactitol-specific enzyme IIC component of PTS (NCBI), at 2,170,945 to 2,172,300
gatC
b2093: gatB - galactitol-specific enzyme IIB component of PTS (NCBI), at 2,172,304 to 2,172,588
gatB
b2094: gatA - galactitol-specific enzyme IIA component of PTS (NCBI), at 2,172,619 to 2,173,071
gatA
b2095: gatZ - D-tagatose 1,6-bisphosphate aldolase 2, subunit (NCBI), at 2,173,081 to 2,174,343
gatZ
b2096: gatY - tagatose-bisphosphate aldolase 1 (VIMSS), at 2,174,372 to 2,175,226
gatY
Position (kb)
2173
2174
2175 Strain fitness (log2 ratio)
-1
0
1
2 at 2172.130 kb on + strand, within gatC at 2172.137 kb on - strand, within gatC at 2172.247 kb on + strand at 2172.255 kb on + strand at 2172.285 kb on - strand at 2172.285 kb on - strand at 2172.295 kb on + strand at 2172.296 kb on - strand at 2172.303 kb on - strand at 2172.305 kb on - strand at 2172.315 kb on - strand at 2172.430 kb on - strand, within gatB at 2172.542 kb on - strand, within gatB at 2172.552 kb on - strand, within gatB at 2172.688 kb on - strand, within gatA at 2172.690 kb on - strand, within gatA at 2172.690 kb on - strand, within gatA at 2172.716 kb on - strand, within gatA at 2172.726 kb on - strand, within gatA at 2172.807 kb on - strand, within gatA at 2172.839 kb on - strand, within gatA at 2172.917 kb on - strand, within gatA at 2172.978 kb on - strand, within gatA at 2172.978 kb on - strand, within gatA at 2172.983 kb on + strand, within gatA at 2173.004 kb on - strand, within gatA at 2173.067 kb on + strand at 2173.075 kb on - strand at 2173.177 kb on - strand at 2173.177 kb on - strand at 2173.197 kb on - strand at 2173.197 kb on - strand at 2173.205 kb on - strand at 2173.205 kb on - strand at 2173.233 kb on - strand, within gatZ at 2173.440 kb on - strand, within gatZ at 2173.444 kb on - strand, within gatZ at 2173.451 kb on + strand, within gatZ at 2173.451 kb on + strand, within gatZ at 2173.459 kb on - strand, within gatZ at 2173.459 kb on - strand, within gatZ at 2173.485 kb on - strand, within gatZ at 2173.588 kb on - strand, within gatZ at 2173.638 kb on - strand, within gatZ at 2173.717 kb on - strand, within gatZ at 2173.783 kb on + strand, within gatZ at 2173.791 kb on - strand, within gatZ at 2173.791 kb on - strand, within gatZ at 2173.870 kb on - strand, within gatZ at 2174.277 kb on + strand at 2174.277 kb on + strand at 2174.285 kb on - strand at 2174.285 kb on - strand at 2174.285 kb on - strand at 2174.371 kb on + strand at 2174.422 kb on - strand at 2174.422 kb on - strand at 2174.469 kb on + strand, within gatY at 2174.749 kb on + strand, within gatY at 2174.749 kb on + strand, within gatY at 2175.002 kb on + strand, within gatY at 2175.029 kb on + strand, within gatY at 2175.129 kb on + strand, within gatY at 2175.189 kb on + strand at 2175.226 kb on + strand at 2175.226 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Time24hr remove 2,172,130 + gatC b2092 0.87 +0.5 2,172,137 - gatC b2092 0.88 +0.3 2,172,247 + +1.4 2,172,255 + +0.7 2,172,285 - +0.1 2,172,285 - -0.6 2,172,295 + +2.1 2,172,296 - -0.4 2,172,303 - -0.4 2,172,305 - -0.2 2,172,315 - +0.6 2,172,430 - gatB b2093 0.44 -0.1 2,172,542 - gatB b2093 0.84 +0.1 2,172,552 - gatB b2093 0.87 +0.1 2,172,688 - gatA b2094 0.15 +0.4 2,172,690 - gatA b2094 0.16 -0.3 2,172,690 - gatA b2094 0.16 -0.1 2,172,716 - gatA b2094 0.21 +0.2 2,172,726 - gatA b2094 0.24 +0.2 2,172,807 - gatA b2094 0.42 -0.2 2,172,839 - gatA b2094 0.49 +0.3 2,172,917 - gatA b2094 0.66 -0.0 2,172,978 - gatA b2094 0.79 -0.0 2,172,978 - gatA b2094 0.79 +0.1 2,172,983 + gatA b2094 0.80 +0.5 2,173,004 - gatA b2094 0.85 +0.2 2,173,067 + +0.5 2,173,075 - +0.3 2,173,177 - -0.0 2,173,177 - +0.4 2,173,197 - +0.3 2,173,197 - +0.5 2,173,205 - +0.5 2,173,205 - -0.1 2,173,233 - gatZ b2095 0.12 +0.8 2,173,440 - gatZ b2095 0.28 -0.5 2,173,444 - gatZ b2095 0.29 +0.3 2,173,451 + gatZ b2095 0.29 +0.5 2,173,451 + gatZ b2095 0.29 +0.3 2,173,459 - gatZ b2095 0.30 +0.3 2,173,459 - gatZ b2095 0.30 +0.6 2,173,485 - gatZ b2095 0.32 +0.6 2,173,588 - gatZ b2095 0.40 +0.2 2,173,638 - gatZ b2095 0.44 +0.4 2,173,717 - gatZ b2095 0.50 +1.7 2,173,783 + gatZ b2095 0.56 +1.1 2,173,791 - gatZ b2095 0.56 +0.2 2,173,791 - gatZ b2095 0.56 +0.1 2,173,870 - gatZ b2095 0.62 +0.4 2,174,277 + +1.2 2,174,277 + +0.4 2,174,285 - -0.1 2,174,285 - +0.4 2,174,285 - +0.0 2,174,371 + +0.1 2,174,422 - -0.1 2,174,422 - -0.6 2,174,469 + gatY b2096 0.11 +0.4 2,174,749 + gatY b2096 0.44 +0.4 2,174,749 + gatY b2096 0.44 +0.6 2,175,002 + gatY b2096 0.74 +0.0 2,175,029 + gatY b2096 0.77 +0.4 2,175,129 + gatY b2096 0.89 +0.4 2,175,189 + +0.7 2,175,226 + +0.7 2,175,226 + +0.2
Or see this region's nucleotide sequence