Strain Fitness in Escherichia coli BW25113 around b2074

Experiment: LB Time24hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyegL and ryeC are separated by 180 nucleotidesryeC and ibsA overlap by 103 nucleotidesibsA and ryeD are separated by 235 nucleotidesryeD and ibsB overlap by 99 nucleotidesibsB and b2074 are separated by 278 nucleotidesb2074 and mdtB overlap by 1 nucleotides b2073: yegL - hypothetical protein (NCBI), at 2,150,493 to 2,151,152 yegL b4436: ryeC - Novel sRNA, function unknown; paralogous to the other QUAD sRNA genes unknown (RefSeq), at 2,151,333 to 2,151,475 ryeC b4667: ibsA - toxic membrane protein (RefSeq), at 2,151,373 to 2,151,432 ibsA b4437: ryeD - Novel sRNA, function unknown; paralogous to the other QUAD sRNA genes (RefSeq), at 2,151,668 to 2,151,803 ryeD b4668: ibsB - toxic membrane protein (RefSeq), at 2,151,705 to 2,151,761 ibsB b2074: b2074 - putative membrane protein (VIMSS), at 2,152,040 to 2,153,287 b2074 b2075: mdtB - multidrug efflux system, subunit B (NCBI), at 2,153,287 to 2,156,409 mdtB Position (kb) 2152 2153 2154Strain fitness (log2 ratio) -1 0 1at 2151.046 kb on - strand, within yegLat 2151.104 kb on - strandat 2151.104 kb on - strandat 2151.195 kb on - strandat 2151.277 kb on + strandat 2151.299 kb on - strandat 2151.311 kb on - strandat 2151.339 kb on + strandat 2151.339 kb on - strandat 2151.354 kb on + strand, within ryeCat 2151.354 kb on + strand, within ryeCat 2151.388 kb on - strandat 2151.411 kb on + strandat 2151.411 kb on + strandat 2151.432 kb on - strandat 2151.512 kb on - strandat 2151.671 kb on + strandat 2151.747 kb on + strandat 2151.747 kb on + strandat 2152.197 kb on + strand, within b2074at 2152.228 kb on + strand, within b2074at 2152.253 kb on + strand, within b2074at 2152.447 kb on + strand, within b2074at 2152.677 kb on - strand, within b2074at 2152.677 kb on - strand, within b2074at 2152.748 kb on + strand, within b2074at 2152.748 kb on + strand, within b2074at 2153.105 kb on + strand, within b2074at 2153.105 kb on + strand, within b2074at 2153.113 kb on + strand, within b2074at 2153.113 kb on - strand, within b2074at 2153.113 kb on - strand, within b2074at 2153.115 kb on + strand, within b2074at 2153.343 kb on - strandat 2153.359 kb on - strandat 2153.359 kb on - strandat 2153.415 kb on - strandat 2153.471 kb on + strandat 2153.581 kb on + strandat 2153.581 kb on + strandat 2153.695 kb on + strand, within mdtBat 2153.695 kb on + strand, within mdtBat 2153.753 kb on + strand, within mdtBat 2153.753 kb on + strand, within mdtBat 2153.984 kb on + strand, within mdtBat 2154.025 kb on + strand, within mdtBat 2154.086 kb on + strand, within mdtBat 2154.103 kb on - strand, within mdtBat 2154.231 kb on + strand, within mdtBat 2154.240 kb on - strand, within mdtBat 2154.260 kb on + strand, within mdtBat 2154.260 kb on + strand, within mdtB

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Time24hr
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2,151,046 - yegL b2073 0.84 +0.2
2,151,104 - +0.1
2,151,104 - -0.1
2,151,195 - -0.1
2,151,277 + +0.3
2,151,299 - +0.1
2,151,311 - +0.3
2,151,339 + +0.1
2,151,339 - +0.7
2,151,354 + ryeC b4436 0.15 +0.6
2,151,354 + ryeC b4436 0.15 -0.2
2,151,388 - +0.2
2,151,411 + -0.1
2,151,411 + +0.3
2,151,432 - +0.3
2,151,512 - +1.2
2,151,671 + -0.1
2,151,747 + -0.1
2,151,747 + +0.3
2,152,197 + b2074 b2074 0.13 +0.2
2,152,228 + b2074 b2074 0.15 +0.3
2,152,253 + b2074 b2074 0.17 -0.5
2,152,447 + b2074 b2074 0.33 -0.0
2,152,677 - b2074 b2074 0.51 -1.5
2,152,677 - b2074 b2074 0.51 +0.4
2,152,748 + b2074 b2074 0.57 -0.3
2,152,748 + b2074 b2074 0.57 +0.7
2,153,105 + b2074 b2074 0.85 +1.0
2,153,105 + b2074 b2074 0.85 +0.8
2,153,113 + b2074 b2074 0.86 +0.8
2,153,113 - b2074 b2074 0.86 +1.2
2,153,113 - b2074 b2074 0.86 +0.6
2,153,115 + b2074 b2074 0.86 +0.2
2,153,343 - +0.3
2,153,359 - -0.5
2,153,359 - +0.6
2,153,415 - +0.1
2,153,471 + +0.2
2,153,581 + -0.2
2,153,581 + +0.4
2,153,695 + mdtB b2075 0.13 +0.7
2,153,695 + mdtB b2075 0.13 +0.5
2,153,753 + mdtB b2075 0.15 -0.1
2,153,753 + mdtB b2075 0.15 +0.4
2,153,984 + mdtB b2075 0.22 -0.2
2,154,025 + mdtB b2075 0.24 -0.0
2,154,086 + mdtB b2075 0.26 -0.2
2,154,103 - mdtB b2075 0.26 +0.1
2,154,231 + mdtB b2075 0.30 +0.1
2,154,240 - mdtB b2075 0.31 +0.3
2,154,260 + mdtB b2075 0.31 +0.6
2,154,260 + mdtB b2075 0.31 -0.3

Or see this region's nucleotide sequence