Experiment: LB Time24hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliM and fliN overlap by 4 nucleotides fliN and fliO are separated by 2 nucleotides fliO and fliP overlap by 1 nucleotides fliP and fliQ are separated by 9 nucleotides fliQ and fliR are separated by 7 nucleotides
b1945: fliM - flagellar motor switch protein M (NCBI), at 2,018,111 to 2,019,115
fliM
b1946: fliN - flagellar motor switch protein (NCBI), at 2,019,112 to 2,019,525
fliN
b1947: fliO - flagellar biosynthesis (VIMSS), at 2,019,528 to 2,019,893
fliO
b1948: fliP - flagellar biosynthesis protein P (NCBI), at 2,019,893 to 2,020,630
fliP
b1949: fliQ - flagellar biosynthesis protein Q (NCBI), at 2,020,640 to 2,020,909
fliQ
b1950: fliR - flagellar biosynthesis protein R (NCBI), at 2,020,917 to 2,021,702
fliR
Position (kb)
2019
2020
2021 Strain fitness (log2 ratio)
-1
0
1
2 at 2019.025 kb on + strand at 2019.106 kb on + strand at 2019.397 kb on + strand, within fliN at 2019.437 kb on + strand, within fliN at 2019.538 kb on + strand at 2019.538 kb on + strand at 2019.995 kb on + strand, within fliP at 2020.000 kb on + strand, within fliP at 2020.231 kb on - strand, within fliP at 2020.359 kb on - strand, within fliP at 2020.359 kb on - strand, within fliP at 2020.375 kb on + strand, within fliP at 2020.382 kb on - strand, within fliP at 2020.668 kb on + strand, within fliQ at 2020.719 kb on - strand, within fliQ at 2020.840 kb on + strand, within fliQ at 2020.894 kb on + strand at 2020.899 kb on - strand at 2020.950 kb on - strand at 2021.298 kb on + strand, within fliR at 2021.377 kb on + strand, within fliR at 2021.377 kb on + strand, within fliR at 2021.413 kb on - strand, within fliR at 2021.498 kb on + strand, within fliR at 2021.501 kb on + strand, within fliR at 2021.501 kb on + strand, within fliR at 2021.522 kb on - strand, within fliR at 2021.572 kb on + strand, within fliR at 2021.594 kb on + strand, within fliR at 2021.594 kb on + strand, within fliR at 2021.595 kb on + strand, within fliR at 2021.595 kb on + strand, within fliR
Per-strain Table
Position Strand Gene LocusTag Fraction LB Time24hr remove 2,019,025 + +0.2 2,019,106 + -0.3 2,019,397 + fliN b1946 0.69 +0.4 2,019,437 + fliN b1946 0.79 -0.1 2,019,538 + +0.1 2,019,538 + +0.7 2,019,995 + fliP b1948 0.14 +0.3 2,020,000 + fliP b1948 0.14 +0.5 2,020,231 - fliP b1948 0.46 +0.4 2,020,359 - fliP b1948 0.63 +0.2 2,020,359 - fliP b1948 0.63 +0.6 2,020,375 + fliP b1948 0.65 +0.9 2,020,382 - fliP b1948 0.66 -0.1 2,020,668 + fliQ b1949 0.10 +0.5 2,020,719 - fliQ b1949 0.29 +0.1 2,020,840 + fliQ b1949 0.74 -0.0 2,020,894 + -0.4 2,020,899 - -0.1 2,020,950 - +0.1 2,021,298 + fliR b1950 0.48 -0.7 2,021,377 + fliR b1950 0.59 +0.3 2,021,377 + fliR b1950 0.59 +0.8 2,021,413 - fliR b1950 0.63 +1.1 2,021,498 + fliR b1950 0.74 -1.0 2,021,501 + fliR b1950 0.74 +0.2 2,021,501 + fliR b1950 0.74 +0.7 2,021,522 - fliR b1950 0.77 +0.6 2,021,572 + fliR b1950 0.83 +2.2 2,021,594 + fliR b1950 0.86 +2.2 2,021,594 + fliR b1950 0.86 +0.6 2,021,595 + fliR b1950 0.86 +1.6 2,021,595 + fliR b1950 0.86 -0.0
Or see this region's nucleotide sequence