Strain Fitness in Escherichia coli BW25113 around b3264

Experiment: LB + carbenicillin Time9hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyhdJ and yhdU are separated by 83 nucleotidesyhdU and envR are separated by 2 nucleotidesenvR and acrE are separated by 398 nucleotides b3262: yhdJ - putative methyltransferase (VIMSS), at 3,409,675 to 3,410,559 yhdJ b3263: yhdU - predicted membrane protein (NCBI), at 3,410,643 to 3,410,822 yhdU b3264: envR - DNA-binding transcriptional regulator (NCBI), at 3,410,825 to 3,411,487 envR b3265: acrE - cytoplasmic membrane lipoprotein (NCBI), at 3,411,886 to 3,413,043 acrE Position (kb) 3410 3411 3412Strain fitness (log2 ratio) -1 0 1 2at 3409.828 kb on - strand, within yhdJat 3409.873 kb on - strand, within yhdJat 3409.879 kb on + strand, within yhdJat 3409.879 kb on + strand, within yhdJat 3409.895 kb on - strand, within yhdJat 3409.915 kb on + strand, within yhdJat 3409.968 kb on + strand, within yhdJat 3410.048 kb on - strand, within yhdJat 3410.097 kb on + strand, within yhdJat 3410.108 kb on + strand, within yhdJat 3410.168 kb on + strand, within yhdJat 3410.186 kb on - strand, within yhdJat 3410.186 kb on - strand, within yhdJat 3410.316 kb on + strand, within yhdJat 3410.334 kb on - strand, within yhdJat 3410.423 kb on + strand, within yhdJat 3410.460 kb on + strand, within yhdJat 3410.464 kb on + strand, within yhdJat 3410.565 kb on + strandat 3410.565 kb on + strandat 3410.565 kb on + strandat 3410.603 kb on + strandat 3410.613 kb on + strandat 3410.616 kb on - strandat 3410.618 kb on + strandat 3410.629 kb on + strandat 3410.631 kb on - strandat 3410.631 kb on - strandat 3410.633 kb on + strandat 3410.633 kb on + strandat 3410.633 kb on + strandat 3410.636 kb on - strandat 3410.653 kb on + strandat 3410.653 kb on + strandat 3410.654 kb on + strandat 3410.661 kb on - strand, within yhdUat 3410.677 kb on - strand, within yhdUat 3410.678 kb on - strand, within yhdUat 3410.678 kb on - strand, within yhdUat 3410.679 kb on + strand, within yhdUat 3410.693 kb on + strand, within yhdUat 3410.698 kb on + strand, within yhdUat 3410.712 kb on - strand, within yhdUat 3410.716 kb on + strand, within yhdUat 3410.716 kb on + strand, within yhdUat 3410.764 kb on + strand, within yhdUat 3410.764 kb on + strand, within yhdUat 3410.764 kb on + strand, within yhdUat 3410.772 kb on - strand, within yhdUat 3410.772 kb on - strand, within yhdUat 3410.778 kb on + strand, within yhdUat 3410.788 kb on + strand, within yhdUat 3410.788 kb on + strand, within yhdUat 3410.788 kb on + strand, within yhdUat 3410.788 kb on + strand, within yhdUat 3410.880 kb on + strandat 3410.896 kb on + strand, within envRat 3410.901 kb on + strand, within envRat 3410.911 kb on + strand, within envRat 3410.968 kb on - strand, within envRat 3411.043 kb on + strand, within envRat 3411.062 kb on + strand, within envRat 3411.079 kb on + strand, within envRat 3411.114 kb on + strand, within envRat 3411.122 kb on - strand, within envRat 3411.231 kb on - strand, within envRat 3411.243 kb on - strand, within envRat 3411.249 kb on - strand, within envRat 3411.250 kb on - strand, within envRat 3411.250 kb on - strand, within envRat 3411.252 kb on + strand, within envRat 3411.260 kb on - strand, within envRat 3411.260 kb on - strand, within envRat 3411.293 kb on + strand, within envRat 3411.298 kb on - strand, within envRat 3411.298 kb on - strand, within envRat 3411.306 kb on - strand, within envRat 3411.369 kb on - strand, within envRat 3411.397 kb on + strand, within envRat 3411.404 kb on + strand, within envRat 3411.410 kb on + strand, within envRat 3411.442 kb on + strandat 3411.442 kb on + strandat 3411.450 kb on + strandat 3411.452 kb on + strandat 3411.455 kb on + strandat 3411.807 kb on - strandat 3411.807 kb on - strandat 3411.807 kb on - strandat 3411.889 kb on - strandat 3411.891 kb on - strandat 3411.923 kb on - strandat 3411.928 kb on - strandat 3411.928 kb on - strandat 3411.928 kb on - strandat 3411.928 kb on - strandat 3411.947 kb on - strandat 3411.960 kb on + strandat 3411.960 kb on + strandat 3411.968 kb on - strandat 3411.999 kb on - strandat 3412.013 kb on + strand, within acrEat 3412.056 kb on - strand, within acrEat 3412.065 kb on + strand, within acrEat 3412.065 kb on + strand, within acrEat 3412.067 kb on - strand, within acrEat 3412.067 kb on - strand, within acrEat 3412.067 kb on - strand, within acrEat 3412.067 kb on - strand, within acrEat 3412.079 kb on - strand, within acrEat 3412.079 kb on - strand, within acrEat 3412.081 kb on - strand, within acrEat 3412.103 kb on - strand, within acrEat 3412.111 kb on - strand, within acrEat 3412.147 kb on - strand, within acrEat 3412.150 kb on + strand, within acrEat 3412.161 kb on + strand, within acrEat 3412.161 kb on + strand, within acrEat 3412.169 kb on - strand, within acrEat 3412.169 kb on - strand, within acrEat 3412.169 kb on - strand, within acrEat 3412.282 kb on + strand, within acrEat 3412.288 kb on + strand, within acrEat 3412.288 kb on + strand, within acrEat 3412.305 kb on + strand, within acrEat 3412.314 kb on - strand, within acrEat 3412.333 kb on + strand, within acrE

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + carbenicillin Time9hr
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3,409,828 - yhdJ b3262 0.17 +0.3
3,409,873 - yhdJ b3262 0.22 +0.3
3,409,879 + yhdJ b3262 0.23 -0.5
3,409,879 + yhdJ b3262 0.23 +0.6
3,409,895 - yhdJ b3262 0.25 -0.2
3,409,915 + yhdJ b3262 0.27 +0.0
3,409,968 + yhdJ b3262 0.33 +0.1
3,410,048 - yhdJ b3262 0.42 +0.3
3,410,097 + yhdJ b3262 0.48 +0.4
3,410,108 + yhdJ b3262 0.49 -0.6
3,410,168 + yhdJ b3262 0.56 +0.2
3,410,186 - yhdJ b3262 0.58 -0.2
3,410,186 - yhdJ b3262 0.58 +0.2
3,410,316 + yhdJ b3262 0.72 +0.1
3,410,334 - yhdJ b3262 0.74 +0.9
3,410,423 + yhdJ b3262 0.85 +0.2
3,410,460 + yhdJ b3262 0.89 -0.1
3,410,464 + yhdJ b3262 0.89 +0.4
3,410,565 + +0.4
3,410,565 + -0.1
3,410,565 + +0.2
3,410,603 + +0.8
3,410,613 + +0.8
3,410,616 - +0.3
3,410,618 + +0.4
3,410,629 + -0.2
3,410,631 - +0.1
3,410,631 - +0.2
3,410,633 + +0.2
3,410,633 + -0.1
3,410,633 + +0.2
3,410,636 - +0.8
3,410,653 + +0.4
3,410,653 + +0.5
3,410,654 + -0.4
3,410,661 - yhdU b3263 0.10 +0.9
3,410,677 - yhdU b3263 0.19 +0.2
3,410,678 - yhdU b3263 0.19 -0.2
3,410,678 - yhdU b3263 0.19 +0.9
3,410,679 + yhdU b3263 0.20 +0.6
3,410,693 + yhdU b3263 0.28 +0.4
3,410,698 + yhdU b3263 0.31 +0.1
3,410,712 - yhdU b3263 0.38 +1.2
3,410,716 + yhdU b3263 0.41 +0.6
3,410,716 + yhdU b3263 0.41 -0.1
3,410,764 + yhdU b3263 0.67 -0.5
3,410,764 + yhdU b3263 0.67 +0.5
3,410,764 + yhdU b3263 0.67 -0.9
3,410,772 - yhdU b3263 0.72 +0.9
3,410,772 - yhdU b3263 0.72 +0.9
3,410,778 + yhdU b3263 0.75 -0.2
3,410,788 + yhdU b3263 0.81 +0.0
3,410,788 + yhdU b3263 0.81 +0.2
3,410,788 + yhdU b3263 0.81 +0.5
3,410,788 + yhdU b3263 0.81 +0.2
3,410,880 + +0.9
3,410,896 + envR b3264 0.11 -1.0
3,410,901 + envR b3264 0.11 -0.1
3,410,911 + envR b3264 0.13 +2.1
3,410,968 - envR b3264 0.22 +0.9
3,411,043 + envR b3264 0.33 +0.9
3,411,062 + envR b3264 0.36 +0.9
3,411,079 + envR b3264 0.38 +0.9
3,411,114 + envR b3264 0.44 +0.9
3,411,122 - envR b3264 0.45 +0.9
3,411,231 - envR b3264 0.61 -0.3
3,411,243 - envR b3264 0.63 +0.5
3,411,249 - envR b3264 0.64 +0.5
3,411,250 - envR b3264 0.64 +0.9
3,411,250 - envR b3264 0.64 +0.3
3,411,252 + envR b3264 0.64 +0.9
3,411,260 - envR b3264 0.66 -0.3
3,411,260 - envR b3264 0.66 +0.3
3,411,293 + envR b3264 0.71 +0.9
3,411,298 - envR b3264 0.71 +0.6
3,411,298 - envR b3264 0.71 +0.7
3,411,306 - envR b3264 0.73 +0.6
3,411,369 - envR b3264 0.82 +0.9
3,411,397 + envR b3264 0.86 +1.3
3,411,404 + envR b3264 0.87 +1.0
3,411,410 + envR b3264 0.88 +1.5
3,411,442 + +0.3
3,411,442 + -0.5
3,411,450 + +0.9
3,411,452 + +1.5
3,411,455 + +0.9
3,411,807 - -0.9
3,411,807 - +0.9
3,411,807 - +0.1
3,411,889 - +1.1
3,411,891 - +0.3
3,411,923 - +0.0
3,411,928 - +1.2
3,411,928 - +0.6
3,411,928 - +0.4
3,411,928 - +0.2
3,411,947 - +0.6
3,411,960 + +0.9
3,411,960 + +0.9
3,411,968 - -0.4
3,411,999 - +0.3
3,412,013 + acrE b3265 0.11 +1.0
3,412,056 - acrE b3265 0.15 -0.0
3,412,065 + acrE b3265 0.15 +0.9
3,412,065 + acrE b3265 0.15 -0.9
3,412,067 - acrE b3265 0.16 +0.3
3,412,067 - acrE b3265 0.16 +0.5
3,412,067 - acrE b3265 0.16 -0.1
3,412,067 - acrE b3265 0.16 -0.1
3,412,079 - acrE b3265 0.17 -0.1
3,412,079 - acrE b3265 0.17 +0.2
3,412,081 - acrE b3265 0.17 +0.2
3,412,103 - acrE b3265 0.19 +0.2
3,412,111 - acrE b3265 0.19 +0.3
3,412,147 - acrE b3265 0.23 +0.2
3,412,150 + acrE b3265 0.23 +0.6
3,412,161 + acrE b3265 0.24 +0.3
3,412,161 + acrE b3265 0.24 -0.2
3,412,169 - acrE b3265 0.24 -0.0
3,412,169 - acrE b3265 0.24 +0.3
3,412,169 - acrE b3265 0.24 +0.2
3,412,282 + acrE b3265 0.34 -0.2
3,412,288 + acrE b3265 0.35 -0.2
3,412,288 + acrE b3265 0.35 +0.1
3,412,305 + acrE b3265 0.36 +0.7
3,412,314 - acrE b3265 0.37 -0.4
3,412,333 + acrE b3265 0.39 -0.2

Or see this region's nucleotide sequence