Strain Fitness in Escherichia coli BW25113 around b2486

Experiment: LB + carbenicillin Time9hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthyfD and hyfE are separated by 11 nucleotideshyfE and hyfF are separated by 4 nucleotideshyfF and hyfG overlap by 11 nucleotides b2484: hyfD - hydrogenase 4 membrane subunit (NCBI), at 2,602,833 to 2,604,272 hyfD b2485: hyfE - hydrogenase 4, membrane subunit (NCBI), at 2,604,284 to 2,604,934 hyfE b2486: hyfF - NADH dehydrogenase subunit N (NCBI), at 2,604,939 to 2,606,519 hyfF b2487: hyfG - hydrogenase 4, subunit (NCBI), at 2,606,509 to 2,608,176 hyfG Position (kb) 2604 2605 2606 2607Strain fitness (log2 ratio) -1 0 1at 2604.064 kb on - strand, within hyfDat 2604.064 kb on - strand, within hyfDat 2604.216 kb on + strandat 2604.216 kb on + strandat 2604.284 kb on - strandat 2604.284 kb on - strandat 2604.340 kb on + strandat 2604.488 kb on + strand, within hyfEat 2604.488 kb on + strand, within hyfEat 2604.513 kb on - strand, within hyfEat 2604.598 kb on - strand, within hyfEat 2604.626 kb on + strand, within hyfEat 2604.700 kb on + strand, within hyfEat 2604.708 kb on - strand, within hyfEat 2604.708 kb on - strand, within hyfEat 2604.828 kb on + strand, within hyfEat 2604.862 kb on - strand, within hyfEat 2604.882 kb on + strandat 2604.882 kb on + strandat 2605.055 kb on - strandat 2605.065 kb on - strandat 2605.065 kb on - strandat 2605.071 kb on - strandat 2605.140 kb on - strand, within hyfFat 2605.210 kb on + strand, within hyfFat 2605.256 kb on + strand, within hyfFat 2605.289 kb on - strand, within hyfFat 2605.374 kb on + strand, within hyfFat 2605.374 kb on + strand, within hyfFat 2605.374 kb on + strand, within hyfFat 2605.420 kb on - strand, within hyfFat 2605.439 kb on - strand, within hyfFat 2605.439 kb on - strand, within hyfFat 2605.478 kb on + strand, within hyfFat 2605.478 kb on + strand, within hyfFat 2605.478 kb on + strand, within hyfFat 2605.481 kb on + strand, within hyfFat 2605.521 kb on - strand, within hyfFat 2605.579 kb on + strand, within hyfFat 2605.601 kb on - strand, within hyfFat 2605.601 kb on - strand, within hyfFat 2605.686 kb on - strand, within hyfFat 2605.686 kb on - strand, within hyfFat 2605.829 kb on - strand, within hyfFat 2605.969 kb on + strand, within hyfFat 2605.969 kb on + strand, within hyfFat 2606.180 kb on + strand, within hyfFat 2606.240 kb on - strand, within hyfFat 2606.420 kb on - strandat 2606.450 kb on + strandat 2606.611 kb on - strandat 2606.648 kb on + strandat 2606.761 kb on - strand, within hyfGat 2606.893 kb on - strand, within hyfGat 2606.922 kb on + strand, within hyfGat 2606.922 kb on + strand, within hyfGat 2606.990 kb on + strand, within hyfGat 2606.998 kb on - strand, within hyfGat 2606.998 kb on - strand, within hyfGat 2607.008 kb on - strand, within hyfGat 2607.199 kb on + strand, within hyfGat 2607.250 kb on + strand, within hyfGat 2607.250 kb on + strand, within hyfGat 2607.258 kb on + strand, within hyfGat 2607.260 kb on + strand, within hyfGat 2607.264 kb on + strand, within hyfGat 2607.272 kb on - strand, within hyfGat 2607.354 kb on + strand, within hyfGat 2607.362 kb on + strand, within hyfGat 2607.397 kb on - strand, within hyfG

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + carbenicillin Time9hr
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2,604,064 - hyfD b2484 0.85 +0.1
2,604,064 - hyfD b2484 0.85 +0.1
2,604,216 + +0.7
2,604,216 + +1.0
2,604,284 - +0.4
2,604,284 - +0.2
2,604,340 + -0.2
2,604,488 + hyfE b2485 0.31 +0.3
2,604,488 + hyfE b2485 0.31 +0.1
2,604,513 - hyfE b2485 0.35 -0.4
2,604,598 - hyfE b2485 0.48 +0.0
2,604,626 + hyfE b2485 0.53 -0.2
2,604,700 + hyfE b2485 0.64 +0.2
2,604,708 - hyfE b2485 0.65 -0.3
2,604,708 - hyfE b2485 0.65 +0.3
2,604,828 + hyfE b2485 0.84 -0.0
2,604,862 - hyfE b2485 0.89 +0.3
2,604,882 + +0.2
2,604,882 + +0.4
2,605,055 - -0.0
2,605,065 - +0.4
2,605,065 - -0.2
2,605,071 - -0.0
2,605,140 - hyfF b2486 0.13 +0.6
2,605,210 + hyfF b2486 0.17 +0.3
2,605,256 + hyfF b2486 0.20 +0.1
2,605,289 - hyfF b2486 0.22 +0.3
2,605,374 + hyfF b2486 0.28 -0.0
2,605,374 + hyfF b2486 0.28 +0.3
2,605,374 + hyfF b2486 0.28 +0.1
2,605,420 - hyfF b2486 0.30 +0.4
2,605,439 - hyfF b2486 0.32 +0.4
2,605,439 - hyfF b2486 0.32 +1.5
2,605,478 + hyfF b2486 0.34 -0.2
2,605,478 + hyfF b2486 0.34 +0.2
2,605,478 + hyfF b2486 0.34 +0.2
2,605,481 + hyfF b2486 0.34 +0.3
2,605,521 - hyfF b2486 0.37 +0.1
2,605,579 + hyfF b2486 0.40 -0.2
2,605,601 - hyfF b2486 0.42 +1.2
2,605,601 - hyfF b2486 0.42 +0.1
2,605,686 - hyfF b2486 0.47 +0.8
2,605,686 - hyfF b2486 0.47 +0.4
2,605,829 - hyfF b2486 0.56 +0.5
2,605,969 + hyfF b2486 0.65 +1.1
2,605,969 + hyfF b2486 0.65 +0.5
2,606,180 + hyfF b2486 0.78 +0.7
2,606,240 - hyfF b2486 0.82 -0.6
2,606,420 - +0.1
2,606,450 + +0.2
2,606,611 - +0.1
2,606,648 + +0.3
2,606,761 - hyfG b2487 0.15 +0.1
2,606,893 - hyfG b2487 0.23 +0.0
2,606,922 + hyfG b2487 0.25 +0.3
2,606,922 + hyfG b2487 0.25 +0.1
2,606,990 + hyfG b2487 0.29 +0.7
2,606,998 - hyfG b2487 0.29 -0.5
2,606,998 - hyfG b2487 0.29 -0.0
2,607,008 - hyfG b2487 0.30 +0.4
2,607,199 + hyfG b2487 0.41 +0.0
2,607,250 + hyfG b2487 0.44 +0.3
2,607,250 + hyfG b2487 0.44 +0.6
2,607,258 + hyfG b2487 0.45 -0.1
2,607,260 + hyfG b2487 0.45 +0.0
2,607,264 + hyfG b2487 0.45 +0.3
2,607,272 - hyfG b2487 0.46 +0.1
2,607,354 + hyfG b2487 0.51 -0.1
2,607,362 + hyfG b2487 0.51 +0.4
2,607,397 - hyfG b2487 0.53 +0.9

Or see this region's nucleotide sequence