Experiment: LB + ciprofloxacin regrowth after 48hrs
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ytfJ and ytfK are separated by 324 nucleotides ytfK and ytfL are separated by 78 nucleotides ytfL and msrA are separated by 322 nucleotides
b4216: ytfJ - predicted transcriptional regulator (NCBI), at 4,436,731 to 4,437,285
ytfJ
b4217: ytfK - orf, hypothetical protein (VIMSS), at 4,437,610 to 4,437,816
ytfK
b4218: ytfL - predicted inner membrane protein (NCBI), at 4,437,895 to 4,439,238
ytfL
b4219: msrA - methionine sulfoxide reductase A (NCBI), at 4,439,561 to 4,440,199
msrA
Position (kb)
4437
4438
4439
4440 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4436.902 kb on - strand, within ytfJ at 4436.902 kb on - strand, within ytfJ at 4436.929 kb on + strand, within ytfJ at 4436.991 kb on + strand, within ytfJ at 4437.003 kb on + strand, within ytfJ at 4437.203 kb on - strand, within ytfJ at 4437.233 kb on + strand at 4437.239 kb on + strand at 4437.247 kb on + strand at 4437.251 kb on + strand at 4437.281 kb on + strand at 4437.281 kb on + strand at 4437.282 kb on - strand at 4437.282 kb on - strand at 4437.287 kb on + strand at 4437.287 kb on - strand at 4437.289 kb on - strand at 4437.289 kb on - strand at 4437.289 kb on - strand at 4437.300 kb on - strand at 4437.316 kb on - strand at 4437.323 kb on + strand at 4437.363 kb on - strand at 4437.363 kb on - strand at 4437.395 kb on + strand at 4437.420 kb on + strand at 4437.420 kb on + strand at 4437.425 kb on + strand at 4437.519 kb on + strand at 4437.526 kb on - strand at 4437.526 kb on - strand at 4437.527 kb on - strand at 4437.527 kb on - strand at 4437.574 kb on + strand at 4437.635 kb on + strand, within ytfK at 4437.635 kb on + strand, within ytfK at 4437.660 kb on - strand, within ytfK at 4437.686 kb on - strand, within ytfK at 4437.686 kb on - strand, within ytfK at 4437.692 kb on - strand, within ytfK at 4437.717 kb on + strand, within ytfK at 4437.768 kb on + strand, within ytfK at 4437.768 kb on + strand, within ytfK at 4437.786 kb on + strand, within ytfK at 4437.788 kb on - strand, within ytfK at 4437.858 kb on - strand at 4437.858 kb on - strand at 4437.860 kb on + strand at 4437.860 kb on + strand at 4437.946 kb on + strand at 4437.958 kb on + strand at 4437.976 kb on - strand at 4437.979 kb on + strand at 4437.997 kb on + strand at 4437.997 kb on + strand at 4438.030 kb on + strand, within ytfL at 4438.071 kb on + strand, within ytfL at 4438.072 kb on - strand, within ytfL at 4438.081 kb on - strand, within ytfL at 4438.081 kb on - strand, within ytfL at 4438.226 kb on - strand, within ytfL at 4438.226 kb on - strand, within ytfL at 4438.281 kb on + strand, within ytfL at 4438.355 kb on - strand, within ytfL at 4438.355 kb on - strand, within ytfL at 4438.369 kb on - strand, within ytfL at 4438.390 kb on + strand, within ytfL at 4438.390 kb on + strand, within ytfL at 4438.408 kb on - strand, within ytfL at 4438.408 kb on - strand, within ytfL at 4438.431 kb on + strand, within ytfL at 4438.438 kb on + strand, within ytfL at 4438.444 kb on - strand, within ytfL at 4438.458 kb on + strand, within ytfL at 4438.458 kb on + strand, within ytfL at 4438.474 kb on - strand, within ytfL at 4438.510 kb on + strand, within ytfL at 4438.527 kb on + strand, within ytfL at 4438.568 kb on - strand, within ytfL at 4438.595 kb on - strand, within ytfL at 4438.595 kb on - strand, within ytfL at 4438.694 kb on - strand, within ytfL at 4438.702 kb on - strand, within ytfL at 4438.731 kb on + strand, within ytfL at 4438.739 kb on - strand, within ytfL at 4438.739 kb on - strand, within ytfL at 4438.755 kb on + strand, within ytfL at 4438.755 kb on + strand, within ytfL at 4438.762 kb on - strand, within ytfL at 4438.762 kb on - strand, within ytfL at 4438.775 kb on + strand, within ytfL at 4438.775 kb on + strand, within ytfL at 4438.826 kb on - strand, within ytfL at 4438.907 kb on + strand, within ytfL at 4438.907 kb on + strand, within ytfL at 4439.009 kb on + strand, within ytfL at 4439.049 kb on - strand, within ytfL at 4439.071 kb on - strand, within ytfL at 4439.201 kb on - strand at 4439.208 kb on + strand at 4439.212 kb on + strand at 4439.212 kb on + strand at 4439.226 kb on - strand at 4439.345 kb on + strand at 4439.345 kb on + strand at 4439.355 kb on - strand at 4439.370 kb on - strand at 4439.431 kb on - strand at 4439.431 kb on - strand at 4439.466 kb on - strand at 4439.688 kb on + strand, within msrA at 4439.739 kb on + strand, within msrA at 4439.759 kb on + strand, within msrA at 4439.759 kb on + strand, within msrA at 4439.764 kb on + strand, within msrA at 4439.872 kb on + strand, within msrA at 4439.937 kb on - strand, within msrA at 4439.937 kb on - strand, within msrA at 4440.009 kb on + strand, within msrA at 4440.090 kb on + strand, within msrA at 4440.120 kb on - strand, within msrA at 4440.131 kb on - strand, within msrA at 4440.143 kb on - strand at 4440.143 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB + ciprofloxacin regrowth after 48hrs remove 4,436,902 - ytfJ b4216 0.31 +0.1 4,436,902 - ytfJ b4216 0.31 +0.5 4,436,929 + ytfJ b4216 0.36 -0.5 4,436,991 + ytfJ b4216 0.47 +0.6 4,437,003 + ytfJ b4216 0.49 +0.4 4,437,203 - ytfJ b4216 0.85 +0.3 4,437,233 + +0.2 4,437,239 + -0.2 4,437,247 + +0.2 4,437,251 + -0.1 4,437,281 + +0.7 4,437,281 + +0.6 4,437,282 - +0.9 4,437,282 - +0.1 4,437,287 + -0.1 4,437,287 - -0.3 4,437,289 - -0.1 4,437,289 - +0.1 4,437,289 - +0.3 4,437,300 - +0.8 4,437,316 - +0.3 4,437,323 + +0.0 4,437,363 - -0.2 4,437,363 - +0.4 4,437,395 + -0.2 4,437,420 + +0.2 4,437,420 + -0.3 4,437,425 + +0.6 4,437,519 + +0.4 4,437,526 - +0.4 4,437,526 - +0.4 4,437,527 - +0.3 4,437,527 - +0.2 4,437,574 + +0.1 4,437,635 + ytfK b4217 0.12 -0.0 4,437,635 + ytfK b4217 0.12 +0.5 4,437,660 - ytfK b4217 0.24 -0.0 4,437,686 - ytfK b4217 0.37 -0.1 4,437,686 - ytfK b4217 0.37 +0.7 4,437,692 - ytfK b4217 0.40 -0.4 4,437,717 + ytfK b4217 0.52 +1.4 4,437,768 + ytfK b4217 0.76 +0.1 4,437,768 + ytfK b4217 0.76 -0.2 4,437,786 + ytfK b4217 0.85 +0.2 4,437,788 - ytfK b4217 0.86 -0.3 4,437,858 - +0.1 4,437,858 - +0.2 4,437,860 + +0.3 4,437,860 + +0.4 4,437,946 + -1.3 4,437,958 + -0.6 4,437,976 - +0.4 4,437,979 + -2.6 4,437,997 + -3.8 4,437,997 + -0.7 4,438,030 + ytfL b4218 0.10 -0.5 4,438,071 + ytfL b4218 0.13 -3.1 4,438,072 - ytfL b4218 0.13 -2.1 4,438,081 - ytfL b4218 0.14 -1.7 4,438,081 - ytfL b4218 0.14 -1.4 4,438,226 - ytfL b4218 0.25 -2.3 4,438,226 - ytfL b4218 0.25 -3.6 4,438,281 + ytfL b4218 0.29 -2.1 4,438,355 - ytfL b4218 0.34 -2.9 4,438,355 - ytfL b4218 0.34 -1.7 4,438,369 - ytfL b4218 0.35 -3.1 4,438,390 + ytfL b4218 0.37 -3.2 4,438,390 + ytfL b4218 0.37 -2.3 4,438,408 - ytfL b4218 0.38 -2.1 4,438,408 - ytfL b4218 0.38 -3.6 4,438,431 + ytfL b4218 0.40 +0.0 4,438,438 + ytfL b4218 0.40 -2.2 4,438,444 - ytfL b4218 0.41 -1.8 4,438,458 + ytfL b4218 0.42 -1.8 4,438,458 + ytfL b4218 0.42 -1.8 4,438,474 - ytfL b4218 0.43 -2.5 4,438,510 + ytfL b4218 0.46 -2.4 4,438,527 + ytfL b4218 0.47 -2.3 4,438,568 - ytfL b4218 0.50 -3.9 4,438,595 - ytfL b4218 0.52 -2.6 4,438,595 - ytfL b4218 0.52 -1.0 4,438,694 - ytfL b4218 0.59 -2.8 4,438,702 - ytfL b4218 0.60 -1.4 4,438,731 + ytfL b4218 0.62 -2.0 4,438,739 - ytfL b4218 0.63 -2.6 4,438,739 - ytfL b4218 0.63 +0.0 4,438,755 + ytfL b4218 0.64 -1.7 4,438,755 + ytfL b4218 0.64 -2.1 4,438,762 - ytfL b4218 0.65 -1.5 4,438,762 - ytfL b4218 0.65 -3.2 4,438,775 + ytfL b4218 0.65 -1.2 4,438,775 + ytfL b4218 0.65 -1.5 4,438,826 - ytfL b4218 0.69 -1.9 4,438,907 + ytfL b4218 0.75 -2.4 4,438,907 + ytfL b4218 0.75 -1.0 4,439,009 + ytfL b4218 0.83 -1.4 4,439,049 - ytfL b4218 0.86 -2.5 4,439,071 - ytfL b4218 0.88 -3.2 4,439,201 - -2.1 4,439,208 + -3.3 4,439,212 + -4.2 4,439,212 + -1.6 4,439,226 - -1.8 4,439,345 + -1.2 4,439,345 + -1.7 4,439,355 - -0.2 4,439,370 - -0.4 4,439,431 - -0.4 4,439,431 - +0.3 4,439,466 - +0.4 4,439,688 + msrA b4219 0.20 +0.1 4,439,739 + msrA b4219 0.28 -0.1 4,439,759 + msrA b4219 0.31 +0.4 4,439,759 + msrA b4219 0.31 +0.3 4,439,764 + msrA b4219 0.32 +0.1 4,439,872 + msrA b4219 0.49 +0.8 4,439,937 - msrA b4219 0.59 +0.0 4,439,937 - msrA b4219 0.59 -0.2 4,440,009 + msrA b4219 0.70 +0.0 4,440,090 + msrA b4219 0.83 +0.2 4,440,120 - msrA b4219 0.87 +0.5 4,440,131 - msrA b4219 0.89 +0.4 4,440,143 - +0.2 4,440,143 - -0.5
Or see this region's nucleotide sequence