Experiment: LB + carbenicillin Time48hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yjeF and yjeE overlap by 29 nucleotides yjeE and amiB are separated by 18 nucleotides amiB and mutL are separated by 9 nucleotides
b4167: yjeF - predicted carbohydrate kinase (NCBI), at 4,392,089 to 4,393,636
yjeF
b4168: yjeE - ATPase with strong ADP affinity (NCBI), at 4,393,608 to 4,394,069
yjeE
b4169: amiB - N-acetylmuramoyl-l-alanine amidase II (NCBI), at 4,394,088 to 4,395,425
amiB
b4170: mutL - DNA mismatch repair protein (NCBI), at 4,395,435 to 4,397,282
mutL
Position (kb)
4394
4395
4396 Strain fitness (log2 ratio)
-1
0
1 at 4393.194 kb on + strand, within yjeF at 4393.306 kb on - strand, within yjeF at 4393.396 kb on - strand, within yjeF at 4393.446 kb on + strand, within yjeF at 4393.446 kb on + strand, within yjeF at 4393.468 kb on - strand, within yjeF at 4393.474 kb on + strand, within yjeF at 4393.476 kb on + strand, within yjeF at 4393.476 kb on + strand, within yjeF at 4393.479 kb on - strand, within yjeF at 4393.490 kb on - strand at 4393.497 kb on + strand at 4393.510 kb on + strand at 4393.543 kb on + strand at 4393.566 kb on - strand at 4393.584 kb on + strand at 4394.078 kb on - strand at 4394.123 kb on + strand at 4394.142 kb on + strand at 4394.143 kb on - strand at 4394.232 kb on - strand, within amiB at 4394.232 kb on - strand, within amiB at 4394.244 kb on + strand, within amiB at 4394.257 kb on + strand, within amiB at 4394.257 kb on + strand, within amiB at 4394.294 kb on - strand, within amiB at 4394.294 kb on - strand, within amiB at 4394.345 kb on - strand, within amiB at 4394.352 kb on - strand, within amiB at 4394.352 kb on - strand, within amiB at 4394.353 kb on - strand, within amiB at 4394.389 kb on + strand, within amiB at 4394.424 kb on + strand, within amiB at 4394.424 kb on + strand, within amiB at 4394.454 kb on + strand, within amiB at 4394.553 kb on + strand, within amiB at 4394.553 kb on + strand, within amiB at 4394.603 kb on - strand, within amiB at 4394.603 kb on - strand, within amiB at 4394.606 kb on - strand, within amiB at 4394.606 kb on - strand, within amiB at 4394.642 kb on + strand, within amiB at 4394.650 kb on - strand, within amiB at 4394.755 kb on + strand, within amiB at 4394.798 kb on - strand, within amiB at 4394.832 kb on - strand, within amiB at 4394.832 kb on - strand, within amiB at 4394.850 kb on + strand, within amiB at 4394.856 kb on - strand, within amiB at 4394.856 kb on - strand, within amiB at 4394.912 kb on + strand, within amiB at 4394.917 kb on - strand, within amiB at 4394.939 kb on - strand, within amiB at 4394.939 kb on - strand, within amiB at 4394.942 kb on + strand, within amiB at 4394.943 kb on + strand, within amiB at 4394.944 kb on + strand, within amiB at 4394.944 kb on + strand, within amiB at 4394.951 kb on - strand, within amiB at 4394.951 kb on - strand, within amiB at 4395.041 kb on + strand, within amiB at 4395.080 kb on - strand, within amiB at 4395.080 kb on - strand, within amiB at 4395.112 kb on + strand, within amiB at 4395.112 kb on + strand, within amiB at 4395.116 kb on - strand, within amiB at 4395.214 kb on + strand, within amiB at 4395.217 kb on + strand, within amiB at 4395.246 kb on + strand, within amiB at 4395.286 kb on + strand, within amiB at 4395.307 kb on - strand at 4395.307 kb on - strand at 4395.348 kb on + strand at 4395.348 kb on + strand at 4395.414 kb on - strand at 4395.454 kb on + strand at 4395.596 kb on - strand at 4395.655 kb on + strand, within mutL at 4395.679 kb on + strand, within mutL at 4395.679 kb on + strand, within mutL at 4395.775 kb on + strand, within mutL at 4395.808 kb on - strand, within mutL at 4395.808 kb on - strand, within mutL at 4395.808 kb on - strand, within mutL at 4395.853 kb on + strand, within mutL at 4395.854 kb on + strand, within mutL at 4395.854 kb on + strand, within mutL at 4395.861 kb on - strand, within mutL at 4395.865 kb on + strand, within mutL at 4395.865 kb on + strand, within mutL at 4395.872 kb on - strand, within mutL at 4395.872 kb on - strand, within mutL at 4395.873 kb on - strand, within mutL at 4395.873 kb on - strand, within mutL at 4395.920 kb on + strand, within mutL at 4395.920 kb on + strand, within mutL at 4395.986 kb on - strand, within mutL at 4395.986 kb on - strand, within mutL at 4396.002 kb on + strand, within mutL at 4396.002 kb on + strand, within mutL at 4396.079 kb on + strand, within mutL at 4396.148 kb on - strand, within mutL at 4396.248 kb on + strand, within mutL at 4396.271 kb on + strand, within mutL at 4396.271 kb on + strand, within mutL at 4396.326 kb on - strand, within mutL at 4396.329 kb on - strand, within mutL at 4396.407 kb on + strand, within mutL at 4396.415 kb on + strand, within mutL at 4396.415 kb on - strand, within mutL
Per-strain Table
Position Strand Gene LocusTag Fraction LB + carbenicillin Time48hr remove 4,393,194 + yjeF b4167 0.71 +0.0 4,393,306 - yjeF b4167 0.79 +0.6 4,393,396 - yjeF b4167 0.84 +0.2 4,393,446 + yjeF b4167 0.88 -0.4 4,393,446 + yjeF b4167 0.88 +0.1 4,393,468 - yjeF b4167 0.89 +0.3 4,393,474 + yjeF b4167 0.89 +0.0 4,393,476 + yjeF b4167 0.90 +0.8 4,393,476 + yjeF b4167 0.90 -0.3 4,393,479 - yjeF b4167 0.90 +0.1 4,393,490 - +0.6 4,393,497 + -0.0 4,393,510 + -0.1 4,393,543 + +0.2 4,393,566 - -0.1 4,393,584 + +0.2 4,394,078 - +0.3 4,394,123 + -0.0 4,394,142 + +0.2 4,394,143 - -0.3 4,394,232 - amiB b4169 0.11 -0.2 4,394,232 - amiB b4169 0.11 +0.1 4,394,244 + amiB b4169 0.12 -0.0 4,394,257 + amiB b4169 0.13 +0.5 4,394,257 + amiB b4169 0.13 -0.7 4,394,294 - amiB b4169 0.15 +0.1 4,394,294 - amiB b4169 0.15 -0.2 4,394,345 - amiB b4169 0.19 -0.2 4,394,352 - amiB b4169 0.20 +0.2 4,394,352 - amiB b4169 0.20 -0.0 4,394,353 - amiB b4169 0.20 +0.2 4,394,389 + amiB b4169 0.22 -0.2 4,394,424 + amiB b4169 0.25 -0.3 4,394,424 + amiB b4169 0.25 -0.0 4,394,454 + amiB b4169 0.27 -0.1 4,394,553 + amiB b4169 0.35 +0.6 4,394,553 + amiB b4169 0.35 -0.2 4,394,603 - amiB b4169 0.38 -0.2 4,394,603 - amiB b4169 0.38 -0.6 4,394,606 - amiB b4169 0.39 -0.1 4,394,606 - amiB b4169 0.39 -0.6 4,394,642 + amiB b4169 0.41 +0.3 4,394,650 - amiB b4169 0.42 -0.2 4,394,755 + amiB b4169 0.50 +0.2 4,394,798 - amiB b4169 0.53 -0.5 4,394,832 - amiB b4169 0.56 +0.0 4,394,832 - amiB b4169 0.56 -0.1 4,394,850 + amiB b4169 0.57 +0.2 4,394,856 - amiB b4169 0.57 -0.1 4,394,856 - amiB b4169 0.57 -0.3 4,394,912 + amiB b4169 0.62 +0.0 4,394,917 - amiB b4169 0.62 -0.2 4,394,939 - amiB b4169 0.64 +0.4 4,394,939 - amiB b4169 0.64 +0.4 4,394,942 + amiB b4169 0.64 +0.2 4,394,943 + amiB b4169 0.64 -0.2 4,394,944 + amiB b4169 0.64 +0.8 4,394,944 + amiB b4169 0.64 -0.5 4,394,951 - amiB b4169 0.64 -0.2 4,394,951 - amiB b4169 0.64 +0.7 4,395,041 + amiB b4169 0.71 -0.1 4,395,080 - amiB b4169 0.74 +0.1 4,395,080 - amiB b4169 0.74 -0.1 4,395,112 + amiB b4169 0.77 -0.2 4,395,112 + amiB b4169 0.77 -0.1 4,395,116 - amiB b4169 0.77 -0.6 4,395,214 + amiB b4169 0.84 +0.1 4,395,217 + amiB b4169 0.84 +0.2 4,395,246 + amiB b4169 0.87 -0.4 4,395,286 + amiB b4169 0.90 -0.2 4,395,307 - +0.7 4,395,307 - -0.0 4,395,348 + -0.4 4,395,348 + -0.1 4,395,414 - +0.0 4,395,454 + +0.6 4,395,596 - +0.2 4,395,655 + mutL b4170 0.12 -0.0 4,395,679 + mutL b4170 0.13 -0.1 4,395,679 + mutL b4170 0.13 +0.2 4,395,775 + mutL b4170 0.18 -0.0 4,395,808 - mutL b4170 0.20 -0.1 4,395,808 - mutL b4170 0.20 -0.1 4,395,808 - mutL b4170 0.20 +1.1 4,395,853 + mutL b4170 0.23 -0.1 4,395,854 + mutL b4170 0.23 +0.3 4,395,854 + mutL b4170 0.23 -0.5 4,395,861 - mutL b4170 0.23 +0.1 4,395,865 + mutL b4170 0.23 +0.5 4,395,865 + mutL b4170 0.23 -0.1 4,395,872 - mutL b4170 0.24 +0.0 4,395,872 - mutL b4170 0.24 +0.1 4,395,873 - mutL b4170 0.24 +0.1 4,395,873 - mutL b4170 0.24 +0.0 4,395,920 + mutL b4170 0.26 +0.5 4,395,920 + mutL b4170 0.26 +0.3 4,395,986 - mutL b4170 0.30 +0.4 4,395,986 - mutL b4170 0.30 +0.1 4,396,002 + mutL b4170 0.31 -0.1 4,396,002 + mutL b4170 0.31 +0.2 4,396,079 + mutL b4170 0.35 +0.3 4,396,148 - mutL b4170 0.39 +0.4 4,396,248 + mutL b4170 0.44 -0.1 4,396,271 + mutL b4170 0.45 -0.4 4,396,271 + mutL b4170 0.45 -0.1 4,396,326 - mutL b4170 0.48 +0.3 4,396,329 - mutL b4170 0.48 -0.0 4,396,407 + mutL b4170 0.53 -0.1 4,396,415 + mutL b4170 0.53 -0.7 4,396,415 - mutL b4170 0.53 +0.3
Or see this region's nucleotide sequence