Strain Fitness in Escherichia coli BW25113 around b2433

Experiment: LB + carbenicillin Time48hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntb2431 and yfeY are separated by 95 nucleotidesyfeY and b2433 are separated by 60 nucleotidesb2433 and b2434 overlap by 14 nucleotidesb2434 and amiA are separated by 213 nucleotides b2431: b2431 - orf, hypothetical protein (VIMSS), at 2,547,668 to 2,548,567 b2431 b2432: yfeY - hypothetical protein (NCBI), at 2,548,663 to 2,549,238 yfeY b2433: b2433 - orf, hypothetical protein (VIMSS), at 2,549,299 to 2,549,748 b2433 b2434: b2434 - orf, hypothetical protein (VIMSS), at 2,549,735 to 2,550,160 b2434 b2435: amiA - N-acetylmuramoyl-l-alanine amidase I (NCBI), at 2,550,374 to 2,551,243 amiA Position (kb) 2549 2550Strain fitness (log2 ratio) -1 0 1at 2548.322 kb on - strand, within b2431at 2548.537 kb on - strandat 2548.537 kb on - strandat 2548.895 kb on + strand, within yfeYat 2549.078 kb on + strand, within yfeYat 2549.078 kb on + strand, within yfeYat 2549.210 kb on + strandat 2549.253 kb on - strandat 2549.266 kb on - strandat 2549.348 kb on + strand, within b2433at 2549.388 kb on + strand, within b2433at 2549.645 kb on + strand, within b2433at 2549.680 kb on + strand, within b2433at 2549.680 kb on + strand, within b2433at 2549.746 kb on - strandat 2549.795 kb on + strand, within b2434at 2549.833 kb on - strand, within b2434at 2549.864 kb on + strand, within b2434at 2550.001 kb on + strand, within b2434at 2550.037 kb on + strand, within b2434at 2550.357 kb on + strandat 2550.357 kb on + strandat 2550.381 kb on + strandat 2550.597 kb on - strand, within amiAat 2550.631 kb on - strand, within amiAat 2550.747 kb on + strand, within amiA

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + carbenicillin Time48hr
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2,548,322 - b2431 b2431 0.73 +0.3
2,548,537 - +0.2
2,548,537 - +0.1
2,548,895 + yfeY b2432 0.40 +0.1
2,549,078 + yfeY b2432 0.72 +0.0
2,549,078 + yfeY b2432 0.72 +0.5
2,549,210 + +0.5
2,549,253 - +0.5
2,549,266 - +0.1
2,549,348 + b2433 b2433 0.11 +0.1
2,549,388 + b2433 b2433 0.20 +0.3
2,549,645 + b2433 b2433 0.77 +0.2
2,549,680 + b2433 b2433 0.85 +1.5
2,549,680 + b2433 b2433 0.85 +1.4
2,549,746 - -0.1
2,549,795 + b2434 b2434 0.14 -1.2
2,549,833 - b2434 b2434 0.23 +0.2
2,549,864 + b2434 b2434 0.30 +0.3
2,550,001 + b2434 b2434 0.62 +0.2
2,550,037 + b2434 b2434 0.71 -0.1
2,550,357 + +0.3
2,550,357 + -0.2
2,550,381 + -0.7
2,550,597 - amiA b2435 0.26 +0.3
2,550,631 - amiA b2435 0.30 +0.4
2,550,747 + amiA b2435 0.43 +0.5

Or see this region's nucleotide sequence