Strain Fitness in Escherichia coli BW25113 around b2294

Experiment: LB + carbenicillin Time48hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyfbS and yfbT are separated by 86 nucleotidesyfbT and b2294 are separated by 10 nucleotidesb2294 and yfbV are separated by 82 nucleotidesyfbV and ackA are separated by 337 nucleotides b2292: yfbS - predicted transporter (NCBI), at 2,407,542 to 2,409,374 yfbS b2293: yfbT - putative phosphatase (VIMSS), at 2,409,461 to 2,410,111 yfbT b2294: b2294 - orf, hypothetical protein (VIMSS), at 2,410,122 to 2,410,616 b2294 b2295: yfbV - hypothetical protein (NCBI), at 2,410,699 to 2,411,154 yfbV b2296: ackA - acetate kinase (NCBI), at 2,411,492 to 2,412,694 ackA Position (kb) 2410 2411Strain fitness (log2 ratio) -1 0 1at 2409.178 kb on + strand, within yfbSat 2409.178 kb on + strand, within yfbSat 2409.195 kb on + strandat 2409.195 kb on + strandat 2409.208 kb on - strandat 2409.251 kb on - strandat 2409.258 kb on - strandat 2409.513 kb on + strandat 2409.636 kb on + strand, within yfbTat 2409.641 kb on + strand, within yfbTat 2409.913 kb on - strand, within yfbTat 2410.159 kb on + strandat 2410.161 kb on - strandat 2410.287 kb on - strand, within b2294at 2410.358 kb on + strand, within b2294at 2410.358 kb on + strand, within b2294at 2410.426 kb on - strand, within b2294at 2410.435 kb on - strand, within b2294at 2410.435 kb on - strand, within b2294at 2410.467 kb on + strand, within b2294at 2410.467 kb on + strand, within b2294at 2410.508 kb on + strand, within b2294at 2410.538 kb on - strand, within b2294at 2410.538 kb on - strand, within b2294at 2410.550 kb on + strand, within b2294at 2410.561 kb on + strand, within b2294at 2410.567 kb on + strandat 2410.567 kb on + strandat 2410.618 kb on + strandat 2410.623 kb on + strandat 2410.636 kb on - strandat 2410.636 kb on - strandat 2410.673 kb on + strandat 2410.673 kb on + strandat 2410.726 kb on - strandat 2410.765 kb on - strand, within yfbVat 2410.808 kb on + strand, within yfbVat 2410.808 kb on + strand, within yfbVat 2410.811 kb on - strand, within yfbVat 2410.858 kb on + strand, within yfbVat 2410.864 kb on + strand, within yfbVat 2410.866 kb on - strand, within yfbVat 2410.886 kb on + strand, within yfbVat 2410.934 kb on - strand, within yfbVat 2410.946 kb on - strand, within yfbVat 2410.948 kb on + strand, within yfbVat 2411.001 kb on + strand, within yfbVat 2411.113 kb on - strandat 2411.138 kb on - strandat 2411.174 kb on - strandat 2411.285 kb on - strandat 2411.290 kb on + strandat 2411.317 kb on + strandat 2411.398 kb on + strandat 2411.398 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + carbenicillin Time48hr
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2,409,178 + yfbS b2292 0.89 +0.0
2,409,178 + yfbS b2292 0.89 +0.1
2,409,195 + -0.2
2,409,195 + -0.1
2,409,208 - +0.2
2,409,251 - -0.3
2,409,258 - +0.1
2,409,513 + -0.1
2,409,636 + yfbT b2293 0.27 +0.2
2,409,641 + yfbT b2293 0.28 +0.2
2,409,913 - yfbT b2293 0.69 +0.1
2,410,159 + +0.1
2,410,161 - +0.1
2,410,287 - b2294 b2294 0.33 +0.0
2,410,358 + b2294 b2294 0.48 +0.2
2,410,358 + b2294 b2294 0.48 +0.9
2,410,426 - b2294 b2294 0.61 +0.2
2,410,435 - b2294 b2294 0.63 +1.3
2,410,435 - b2294 b2294 0.63 +0.2
2,410,467 + b2294 b2294 0.70 +0.5
2,410,467 + b2294 b2294 0.70 +0.5
2,410,508 + b2294 b2294 0.78 -0.3
2,410,538 - b2294 b2294 0.84 -0.0
2,410,538 - b2294 b2294 0.84 +0.5
2,410,550 + b2294 b2294 0.86 +0.2
2,410,561 + b2294 b2294 0.89 +0.3
2,410,567 + -0.0
2,410,567 + -0.0
2,410,618 + +0.8
2,410,623 + +0.3
2,410,636 - +0.6
2,410,636 - +0.5
2,410,673 + -0.5
2,410,673 + +0.6
2,410,726 - -0.2
2,410,765 - yfbV b2295 0.14 -0.2
2,410,808 + yfbV b2295 0.24 +0.1
2,410,808 + yfbV b2295 0.24 +0.0
2,410,811 - yfbV b2295 0.25 +0.2
2,410,858 + yfbV b2295 0.35 -0.3
2,410,864 + yfbV b2295 0.36 +0.2
2,410,866 - yfbV b2295 0.37 -0.5
2,410,886 + yfbV b2295 0.41 -0.2
2,410,934 - yfbV b2295 0.52 -0.0
2,410,946 - yfbV b2295 0.54 -0.1
2,410,948 + yfbV b2295 0.55 +0.1
2,411,001 + yfbV b2295 0.66 +0.9
2,411,113 - +0.4
2,411,138 - -0.2
2,411,174 - +0.1
2,411,285 - +0.4
2,411,290 + +0.5
2,411,317 + +0.4
2,411,398 + +0.3
2,411,398 + -0.2

Or see this region's nucleotide sequence