Strain Fitness in Escherichia coli BW25113 around b1467

Experiment: LB + carbenicillin Time48hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnarV and narW overlap by 4 nucleotidesnarW and narY overlap by 1 nucleotidesnarY and narZ overlap by 4 nucleotides b1465: narV - nitrate reductase 2 (NRZ), gamma subunit (NCBI), at 1,533,961 to 1,534,641 narV b1466: narW - nitrate reductase 2 (NRZ), delta subunit (assembly subunit) (NCBI), at 1,534,638 to 1,535,333 narW b1467: narY - nitrate reductase 2 (NRZ), beta subunit (NCBI), at 1,535,333 to 1,536,877 narY b1468: narZ - nitrate reductase 2 (NRZ), alpha subunit (NCBI), at 1,536,874 to 1,540,614 narZ Position (kb) 1535 1536 1537Strain fitness (log2 ratio) -1 0 1 2at 1534.336 kb on + strand, within narVat 1534.625 kb on + strandat 1534.659 kb on + strandat 1534.659 kb on + strandat 1534.776 kb on + strand, within narWat 1535.009 kb on + strand, within narWat 1535.035 kb on - strand, within narWat 1535.064 kb on - strand, within narWat 1535.075 kb on + strand, within narWat 1535.172 kb on - strand, within narWat 1535.310 kb on + strandat 1535.352 kb on - strandat 1535.352 kb on - strandat 1535.359 kb on + strandat 1535.563 kb on + strand, within narYat 1535.583 kb on - strand, within narYat 1535.843 kb on - strand, within narYat 1535.885 kb on + strand, within narYat 1536.005 kb on - strand, within narYat 1536.005 kb on - strand, within narYat 1536.015 kb on - strand, within narYat 1536.054 kb on + strand, within narYat 1536.316 kb on - strand, within narYat 1536.316 kb on - strand, within narYat 1536.323 kb on + strand, within narYat 1536.895 kb on - strandat 1537.060 kb on - strandat 1537.542 kb on - strand, within narZat 1537.763 kb on - strand, within narZat 1537.763 kb on - strand, within narZat 1537.800 kb on - strand, within narZ

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + carbenicillin Time48hr
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1,534,336 + narV b1465 0.55 +0.6
1,534,625 + +0.6
1,534,659 + -0.0
1,534,659 + -0.7
1,534,776 + narW b1466 0.20 +0.0
1,535,009 + narW b1466 0.53 +0.1
1,535,035 - narW b1466 0.57 +0.3
1,535,064 - narW b1466 0.61 -0.1
1,535,075 + narW b1466 0.63 +0.3
1,535,172 - narW b1466 0.77 +0.6
1,535,310 + +0.2
1,535,352 - +0.3
1,535,352 - +0.4
1,535,359 + -0.1
1,535,563 + narY b1467 0.15 +0.2
1,535,583 - narY b1467 0.16 +0.0
1,535,843 - narY b1467 0.33 +0.2
1,535,885 + narY b1467 0.36 +0.1
1,536,005 - narY b1467 0.43 +0.5
1,536,005 - narY b1467 0.43 +0.4
1,536,015 - narY b1467 0.44 +2.2
1,536,054 + narY b1467 0.47 +0.6
1,536,316 - narY b1467 0.64 +0.3
1,536,316 - narY b1467 0.64 +0.3
1,536,323 + narY b1467 0.64 +0.7
1,536,895 - +0.4
1,537,060 - +0.2
1,537,542 - narZ b1468 0.18 +0.7
1,537,763 - narZ b1468 0.24 +0.8
1,537,763 - narZ b1468 0.24 +0.7
1,537,800 - narZ b1468 0.25 +0.4

Or see this region's nucleotide sequence