Strain Fitness in Escherichia coli BW25113 around b1440

Experiment: LB + carbenicillin Time48hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntydcR and ydcS are separated by 244 nucleotidesydcS and ydcT are separated by 17 nucleotides b1439: ydcR - fused predicted DNA-binding transcriptional regulator/predicted amino transferase (NCBI), at 1,508,027 to 1,509,433 ydcR b1440: ydcS - predicted spermidine/putrescine transporter subunit (NCBI), at 1,509,678 to 1,510,823 ydcS b1441: ydcT - predicted spermidine/putrescine transporter subunit (NCBI), at 1,510,841 to 1,511,854 ydcT Position (kb) 1509 1510 1511Strain fitness (log2 ratio) -1 0 1 2 3at 1508.820 kb on + strand, within ydcRat 1508.820 kb on + strand, within ydcRat 1508.990 kb on - strand, within ydcRat 1509.170 kb on - strand, within ydcRat 1509.170 kb on - strand, within ydcRat 1509.236 kb on + strand, within ydcRat 1509.369 kb on + strandat 1509.375 kb on - strandat 1509.377 kb on - strandat 1509.377 kb on - strandat 1509.674 kb on - strandat 1509.693 kb on + strandat 1509.707 kb on + strandat 1509.718 kb on + strandat 1509.718 kb on + strandat 1509.733 kb on - strandat 1509.809 kb on + strand, within ydcSat 1509.834 kb on + strand, within ydcSat 1509.884 kb on + strand, within ydcSat 1510.004 kb on + strand, within ydcSat 1510.220 kb on + strand, within ydcSat 1510.227 kb on + strand, within ydcSat 1510.336 kb on - strand, within ydcSat 1510.336 kb on - strand, within ydcSat 1510.361 kb on + strand, within ydcSat 1510.390 kb on + strand, within ydcSat 1510.390 kb on + strand, within ydcSat 1510.392 kb on - strand, within ydcSat 1510.439 kb on - strand, within ydcSat 1510.661 kb on + strand, within ydcSat 1510.661 kb on + strand, within ydcSat 1510.763 kb on - strandat 1510.962 kb on - strand, within ydcTat 1511.021 kb on + strand, within ydcTat 1511.073 kb on + strand, within ydcTat 1511.302 kb on + strand, within ydcTat 1511.365 kb on - strand, within ydcTat 1511.428 kb on - strand, within ydcTat 1511.757 kb on - strandat 1511.782 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + carbenicillin Time48hr
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1,508,820 + ydcR b1439 0.56 -0.1
1,508,820 + ydcR b1439 0.56 +0.1
1,508,990 - ydcR b1439 0.68 +3.2
1,509,170 - ydcR b1439 0.81 +0.4
1,509,170 - ydcR b1439 0.81 +0.6
1,509,236 + ydcR b1439 0.86 +0.9
1,509,369 + +0.8
1,509,375 - +0.2
1,509,377 - -0.2
1,509,377 - +0.7
1,509,674 - +0.5
1,509,693 + +0.5
1,509,707 + +0.5
1,509,718 + +0.1
1,509,718 + +0.4
1,509,733 - +0.5
1,509,809 + ydcS b1440 0.11 +0.6
1,509,834 + ydcS b1440 0.14 +0.3
1,509,884 + ydcS b1440 0.18 +0.3
1,510,004 + ydcS b1440 0.28 +0.9
1,510,220 + ydcS b1440 0.47 +0.5
1,510,227 + ydcS b1440 0.48 +0.3
1,510,336 - ydcS b1440 0.57 +0.2
1,510,336 - ydcS b1440 0.57 -0.1
1,510,361 + ydcS b1440 0.60 +0.7
1,510,390 + ydcS b1440 0.62 +0.3
1,510,390 + ydcS b1440 0.62 +0.2
1,510,392 - ydcS b1440 0.62 +0.7
1,510,439 - ydcS b1440 0.66 +0.4
1,510,661 + ydcS b1440 0.86 +1.1
1,510,661 + ydcS b1440 0.86 +0.2
1,510,763 - +0.1
1,510,962 - ydcT b1441 0.12 -0.7
1,511,021 + ydcT b1441 0.18 +0.4
1,511,073 + ydcT b1441 0.23 +0.2
1,511,302 + ydcT b1441 0.45 +0.4
1,511,365 - ydcT b1441 0.52 +0.2
1,511,428 - ydcT b1441 0.58 -0.3
1,511,757 - +0.3
1,511,782 + +0.0

Or see this region's nucleotide sequence