Strain Fitness in Escherichia coli BW25113 around b0407

Experiment: LB + carbenicillin Time48hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttgt and yajC are separated by 22 nucleotidesyajC and secD are separated by 27 nucleotides b0406: tgt - queuine tRNA-ribosyltransferase (NCBI), at 425,361 to 426,488 tgt b0407: yajC - preprotein translocase subunit YajC (NCBI), at 426,511 to 426,843 yajC b0408: secD - protein export protein SecD (NCBI), at 426,871 to 428,718 secD Position (kb) 426 427Strain fitness (log2 ratio) -1 0 1 2at 425.700 kb on - strand, within tgtat 425.700 kb on - strand, within tgtat 425.852 kb on - strand, within tgtat 425.914 kb on + strand, within tgtat 425.945 kb on - strand, within tgtat 425.945 kb on - strandat 425.964 kb on + strand, within tgtat 426.030 kb on - strand, within tgtat 426.030 kb on - strand, within tgtat 426.035 kb on - strand, within tgtat 426.035 kb on - strand, within tgtat 426.230 kb on + strand, within tgtat 426.230 kb on + strand, within tgtat 426.241 kb on + strand, within tgtat 426.249 kb on - strand, within tgtat 426.259 kb on - strand, within tgtat 426.277 kb on - strand, within tgtat 426.318 kb on - strand, within tgtat 426.352 kb on - strand, within tgtat 426.352 kb on - strand, within tgtat 426.352 kb on - strand, within tgtat 426.352 kb on - strand, within tgtat 426.405 kb on + strandat 426.481 kb on - strandat 426.561 kb on + strand, within yajCat 426.666 kb on + strand, within yajCat 426.666 kb on + strand, within yajCat 426.765 kb on + strand, within yajCat 426.765 kb on + strand, within yajC

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + carbenicillin Time48hr
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425,700 - tgt b0406 0.30 +0.3
425,700 - tgt b0406 0.30 +0.7
425,852 - tgt b0406 0.44 -0.4
425,914 + tgt b0406 0.49 +0.3
425,945 - tgt b0406 0.52 +0.3
425,945 - +2.4
425,964 + tgt b0406 0.53 -0.2
426,030 - tgt b0406 0.59 -1.3
426,030 - tgt b0406 0.59 +1.0
426,035 - tgt b0406 0.60 -1.1
426,035 - tgt b0406 0.60 -0.3
426,230 + tgt b0406 0.77 -0.1
426,230 + tgt b0406 0.77 +1.2
426,241 + tgt b0406 0.78 +0.2
426,249 - tgt b0406 0.79 +0.3
426,259 - tgt b0406 0.80 +0.1
426,277 - tgt b0406 0.81 +0.7
426,318 - tgt b0406 0.85 +1.1
426,352 - tgt b0406 0.88 -0.3
426,352 - tgt b0406 0.88 -0.7
426,352 - tgt b0406 0.88 +0.4
426,352 - tgt b0406 0.88 +0.7
426,405 + +0.3
426,481 - +0.4
426,561 + yajC b0407 0.15 -0.0
426,666 + yajC b0407 0.47 +1.0
426,666 + yajC b0407 0.47 -0.1
426,765 + yajC b0407 0.76 +1.0
426,765 + yajC b0407 0.76 +2.3

Or see this region's nucleotide sequence