Strain Fitness in Escherichia coli BW25113 around b3863

Experiment: LB + ciprofloxacin Time24hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyihG and polA are separated by 363 nucleotidespolA and spf are separated by 146 nucleotidesspf and yihA are separated by 125 nucleotides b3862: yihG - predicted endonuclease (NCBI), at 4,043,693 to 4,044,625 yihG b3863: polA - DNA polymerase I (NCBI), at 4,044,989 to 4,047,775 polA b3864: spf - Spot 42 RNA (NCBI), at 4,047,922 to 4,048,030 spf b3865: yihA - GTP-binding protein (RefSeq), at 4,048,156 to 4,048,788 yihA Position (kb) 4044 4045 4046 4047 4048Strain fitness (log2 ratio) -1 0 1 2at 4044.086 kb on - strand, within yihGat 4044.145 kb on - strand, within yihGat 4044.194 kb on - strand, within yihGat 4044.199 kb on - strand, within yihGat 4044.242 kb on - strand, within yihGat 4044.242 kb on - strand, within yihGat 4044.264 kb on + strand, within yihGat 4044.264 kb on + strand, within yihGat 4044.286 kb on - strand, within yihGat 4044.312 kb on + strand, within yihGat 4044.349 kb on + strand, within yihGat 4044.349 kb on + strand, within yihGat 4044.353 kb on - strand, within yihGat 4044.357 kb on - strand, within yihGat 4044.376 kb on + strand, within yihGat 4044.386 kb on - strand, within yihGat 4044.387 kb on + strand, within yihGat 4044.399 kb on - strand, within yihGat 4044.399 kb on - strand, within yihGat 4044.401 kb on + strand, within yihGat 4044.401 kb on + strand, within yihGat 4044.409 kb on + strand, within yihGat 4044.409 kb on - strand, within yihGat 4044.409 kb on - strand, within yihGat 4044.409 kb on - strand, within yihGat 4044.491 kb on - strand, within yihGat 4044.533 kb on - strandat 4044.553 kb on + strandat 4044.592 kb on - strandat 4044.629 kb on - strandat 4044.629 kb on - strandat 4044.629 kb on - strandat 4044.629 kb on - strandat 4044.629 kb on - strandat 4044.890 kb on - strandat 4044.890 kb on - strandat 4044.960 kb on - strandat 4044.962 kb on - strandat 4044.962 kb on - strandat 4044.982 kb on + strandat 4044.985 kb on + strandat 4044.985 kb on - strandat 4044.985 kb on - strandat 4045.938 kb on + strand, within polAat 4046.403 kb on + strand, within polAat 4046.840 kb on + strand, within polAat 4046.907 kb on + strand, within polAat 4047.703 kb on + strandat 4047.740 kb on + strandat 4047.740 kb on + strandat 4047.819 kb on - strandat 4047.920 kb on + strandat 4047.925 kb on + strandat 4048.005 kb on + strand, within spfat 4048.054 kb on - strandat 4048.117 kb on + strandat 4048.201 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + ciprofloxacin Time24hr
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4,044,086 - yihG b3862 0.42 +0.5
4,044,145 - yihG b3862 0.48 +0.2
4,044,194 - yihG b3862 0.54 +0.2
4,044,199 - yihG b3862 0.54 +0.4
4,044,242 - yihG b3862 0.59 -0.0
4,044,242 - yihG b3862 0.59 +0.1
4,044,264 + yihG b3862 0.61 +1.1
4,044,264 + yihG b3862 0.61 -1.0
4,044,286 - yihG b3862 0.64 -0.1
4,044,312 + yihG b3862 0.66 +0.1
4,044,349 + yihG b3862 0.70 +0.1
4,044,349 + yihG b3862 0.70 +2.1
4,044,353 - yihG b3862 0.71 -0.1
4,044,357 - yihG b3862 0.71 +0.9
4,044,376 + yihG b3862 0.73 -0.2
4,044,386 - yihG b3862 0.74 +0.1
4,044,387 + yihG b3862 0.74 +0.4
4,044,399 - yihG b3862 0.76 +0.4
4,044,399 - yihG b3862 0.76 -0.2
4,044,401 + yihG b3862 0.76 -0.3
4,044,401 + yihG b3862 0.76 -0.1
4,044,409 + yihG b3862 0.77 -0.7
4,044,409 - yihG b3862 0.77 -0.2
4,044,409 - yihG b3862 0.77 +0.1
4,044,409 - yihG b3862 0.77 +0.3
4,044,491 - yihG b3862 0.86 +0.1
4,044,533 - +0.5
4,044,553 + +0.1
4,044,592 - +0.1
4,044,629 - +1.1
4,044,629 - +0.2
4,044,629 - +0.2
4,044,629 - -0.3
4,044,629 - +0.6
4,044,890 - +0.3
4,044,890 - +0.1
4,044,960 - +0.3
4,044,962 - +0.8
4,044,962 - +0.6
4,044,982 + -0.3
4,044,985 + -0.1
4,044,985 - +0.1
4,044,985 - +0.8
4,045,938 + polA b3863 0.34 +1.7
4,046,403 + polA b3863 0.51 +1.1
4,046,840 + polA b3863 0.66 -0.5
4,046,907 + polA b3863 0.69 -1.5
4,047,703 + -0.3
4,047,740 + +0.3
4,047,740 + +0.0
4,047,819 - +0.4
4,047,920 + +0.6
4,047,925 + +0.3
4,048,005 + spf b3864 0.76 +0.9
4,048,054 - +0.1
4,048,117 + +0.4
4,048,201 - +0.5

Or see this region's nucleotide sequence