Strain Fitness in Escherichia coli BW25113 around b3099

Experiment: LB + ciprofloxacin Time24hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyqjB and yqjC are separated by 146 nucleotidesyqjC and yqjD are separated by 37 nucleotidesyqjD and yqjE are separated by 2 nucleotidesyqjE and yqjK overlap by 11 nucleotidesyqjK and yqjF are separated by 185 nucleotidesyqjF and yqjG are separated by 69 nucleotides b3096: yqjB - hypothetical protein (NCBI), at 3,246,461 to 3,246,844 yqjB b3097: yqjC - orf, hypothetical protein (VIMSS), at 3,246,991 to 3,247,359 yqjC b3098: yqjD - hypothetical protein (NCBI), at 3,247,397 to 3,247,702 yqjD b3099: yqjE - conserved inner membrane protein (NCBI), at 3,247,705 to 3,248,109 yqjE b3100: yqjK - hypothetical protein (NCBI), at 3,248,099 to 3,248,398 yqjK b3101: yqjF - orf, hypothetical protein (VIMSS), at 3,248,584 to 3,248,976 yqjF b3102: yqjG - predicted S-transferase (NCBI), at 3,249,046 to 3,250,032 yqjG Position (kb) 3247 3248 3249Strain fitness (log2 ratio) -1 0 1at 3246.751 kb on + strand, within yqjBat 3246.760 kb on + strand, within yqjBat 3246.760 kb on + strand, within yqjBat 3246.766 kb on - strand, within yqjBat 3246.781 kb on + strand, within yqjBat 3246.781 kb on + strand, within yqjBat 3246.789 kb on - strand, within yqjBat 3246.799 kb on + strand, within yqjBat 3246.834 kb on + strandat 3246.834 kb on + strandat 3246.873 kb on - strandat 3246.873 kb on - strandat 3246.937 kb on + strandat 3246.961 kb on - strandat 3246.961 kb on - strandat 3247.049 kb on + strand, within yqjCat 3247.054 kb on + strand, within yqjCat 3247.057 kb on - strand, within yqjCat 3247.057 kb on - strand, within yqjCat 3247.063 kb on + strand, within yqjCat 3247.067 kb on - strand, within yqjCat 3247.140 kb on + strand, within yqjCat 3247.140 kb on + strand, within yqjCat 3247.170 kb on + strand, within yqjCat 3247.177 kb on - strand, within yqjCat 3247.257 kb on - strand, within yqjCat 3247.257 kb on - strand, within yqjCat 3247.377 kb on - strandat 3247.382 kb on + strandat 3247.558 kb on + strand, within yqjDat 3247.616 kb on + strand, within yqjDat 3247.616 kb on + strand, within yqjDat 3247.649 kb on + strand, within yqjDat 3247.784 kb on + strand, within yqjEat 3247.801 kb on - strand, within yqjEat 3247.801 kb on - strand, within yqjEat 3247.995 kb on + strand, within yqjEat 3248.030 kb on + strand, within yqjEat 3248.030 kb on + strand, within yqjEat 3248.030 kb on + strand, within yqjEat 3248.041 kb on - strand, within yqjEat 3248.076 kb on + strandat 3248.140 kb on - strand, within yqjKat 3248.140 kb on - strand, within yqjKat 3248.162 kb on + strand, within yqjKat 3248.281 kb on + strand, within yqjKat 3248.281 kb on + strand, within yqjKat 3248.309 kb on - strand, within yqjKat 3248.309 kb on - strand, within yqjKat 3248.323 kb on - strand, within yqjKat 3248.326 kb on + strand, within yqjKat 3248.384 kb on - strandat 3248.676 kb on + strand, within yqjFat 3248.790 kb on + strand, within yqjFat 3248.797 kb on + strand, within yqjFat 3248.940 kb on + strandat 3248.948 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + ciprofloxacin Time24hr
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3,246,751 + yqjB b3096 0.76 -0.1
3,246,760 + yqjB b3096 0.78 -0.0
3,246,760 + yqjB b3096 0.78 -0.2
3,246,766 - yqjB b3096 0.79 +0.3
3,246,781 + yqjB b3096 0.83 +0.1
3,246,781 + yqjB b3096 0.83 +0.4
3,246,789 - yqjB b3096 0.85 +0.4
3,246,799 + yqjB b3096 0.88 +0.2
3,246,834 + +0.6
3,246,834 + -0.0
3,246,873 - +0.1
3,246,873 - -0.2
3,246,937 + +0.1
3,246,961 - +0.5
3,246,961 - -0.0
3,247,049 + yqjC b3097 0.16 +0.3
3,247,054 + yqjC b3097 0.17 +0.4
3,247,057 - yqjC b3097 0.18 -0.2
3,247,057 - yqjC b3097 0.18 +1.2
3,247,063 + yqjC b3097 0.20 +0.5
3,247,067 - yqjC b3097 0.21 -0.5
3,247,140 + yqjC b3097 0.40 +0.3
3,247,140 + yqjC b3097 0.40 -0.3
3,247,170 + yqjC b3097 0.49 +0.1
3,247,177 - yqjC b3097 0.50 +0.2
3,247,257 - yqjC b3097 0.72 -0.4
3,247,257 - yqjC b3097 0.72 -0.2
3,247,377 - +0.3
3,247,382 + +0.5
3,247,558 + yqjD b3098 0.53 +0.3
3,247,616 + yqjD b3098 0.72 -0.2
3,247,616 + yqjD b3098 0.72 -0.0
3,247,649 + yqjD b3098 0.82 +0.6
3,247,784 + yqjE b3099 0.20 +0.2
3,247,801 - yqjE b3099 0.24 +0.2
3,247,801 - yqjE b3099 0.24 +0.3
3,247,995 + yqjE b3099 0.72 -0.0
3,248,030 + yqjE b3099 0.80 +0.4
3,248,030 + yqjE b3099 0.80 +0.2
3,248,030 + yqjE b3099 0.80 +0.8
3,248,041 - yqjE b3099 0.83 +0.9
3,248,076 + -0.2
3,248,140 - yqjK b3100 0.14 +1.4
3,248,140 - yqjK b3100 0.14 +1.0
3,248,162 + yqjK b3100 0.21 +0.7
3,248,281 + yqjK b3100 0.61 +0.3
3,248,281 + yqjK b3100 0.61 +0.4
3,248,309 - yqjK b3100 0.70 +0.5
3,248,309 - yqjK b3100 0.70 +0.3
3,248,323 - yqjK b3100 0.75 +0.1
3,248,326 + yqjK b3100 0.76 -0.3
3,248,384 - -0.5
3,248,676 + yqjF b3101 0.23 +0.1
3,248,790 + yqjF b3101 0.52 +0.4
3,248,797 + yqjF b3101 0.54 -0.0
3,248,940 + +0.4
3,248,948 - +0.2

Or see this region's nucleotide sequence