Strain Fitness in Escherichia coli BW25113 around b2997

Experiment: LB + ciprofloxacin Time24hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthybB and hybA overlap by 11 nucleotideshybA and hybO are separated by 2 nucleotideshybO and yghW are separated by 188 nucleotidesyghW and yghX are separated by 118 nucleotides b2995: hybB - predicted hydrogenase 2 cytochrome b type component (NCBI), at 3,141,008 to 3,142,186 hybB b2996: hybA - hydrogenase 2 4Fe-4S ferredoxin-type component (NCBI), at 3,142,176 to 3,143,162 hybA b2997: hybO - hydrogenase 2, small subunit (NCBI), at 3,143,165 to 3,144,283 hybO b2998: yghW - hypothetical protein (NCBI), at 3,144,472 to 3,144,759 yghW b4658: yghX - no description, at 3,144,878 to 3,145,713 yghX Position (kb) 3143 3144 3145Strain fitness (log2 ratio) -1 0 1at 3142.271 kb on - strandat 3142.284 kb on - strand, within hybAat 3142.284 kb on - strand, within hybAat 3142.284 kb on - strand, within hybAat 3142.286 kb on - strand, within hybAat 3142.286 kb on - strand, within hybAat 3142.368 kb on + strand, within hybAat 3142.410 kb on + strand, within hybAat 3142.446 kb on - strand, within hybAat 3142.585 kb on - strand, within hybAat 3142.585 kb on - strand, within hybAat 3142.587 kb on + strand, within hybAat 3142.600 kb on + strand, within hybAat 3142.676 kb on - strand, within hybAat 3142.719 kb on - strand, within hybAat 3142.719 kb on - strand, within hybAat 3142.865 kb on + strand, within hybAat 3142.877 kb on + strand, within hybAat 3142.885 kb on - strand, within hybAat 3142.995 kb on - strand, within hybAat 3142.995 kb on - strand, within hybAat 3143.008 kb on + strand, within hybAat 3143.108 kb on - strandat 3143.108 kb on - strandat 3143.127 kb on + strandat 3143.127 kb on + strandat 3143.223 kb on + strandat 3143.230 kb on + strandat 3143.230 kb on + strandat 3143.277 kb on - strand, within hybOat 3143.279 kb on - strand, within hybOat 3143.410 kb on - strand, within hybOat 3143.410 kb on - strand, within hybOat 3143.456 kb on + strand, within hybOat 3143.471 kb on - strand, within hybOat 3143.475 kb on + strand, within hybOat 3143.499 kb on - strand, within hybOat 3143.515 kb on - strand, within hybOat 3143.521 kb on - strand, within hybOat 3143.656 kb on + strand, within hybOat 3143.677 kb on - strandat 3143.677 kb on - strand, within hybOat 3143.799 kb on - strand, within hybOat 3143.861 kb on - strand, within hybOat 3143.897 kb on + strand, within hybOat 3143.960 kb on - strand, within hybOat 3143.995 kb on + strand, within hybOat 3144.007 kb on + strand, within hybOat 3144.091 kb on + strand, within hybOat 3144.105 kb on + strand, within hybOat 3144.105 kb on + strand, within hybOat 3144.201 kb on + strandat 3144.202 kb on - strandat 3144.250 kb on + strandat 3144.259 kb on + strandat 3144.259 kb on + strandat 3144.267 kb on + strandat 3144.269 kb on + strandat 3144.269 kb on + strandat 3144.395 kb on + strandat 3144.395 kb on + strandat 3144.398 kb on - strandat 3144.437 kb on - strandat 3144.485 kb on + strandat 3144.553 kb on + strand, within yghWat 3144.553 kb on + strand, within yghWat 3144.553 kb on + strand, within yghWat 3144.553 kb on + strand, within yghWat 3144.553 kb on + strand, within yghWat 3144.560 kb on - strand, within yghWat 3144.561 kb on - strand, within yghWat 3144.564 kb on + strand, within yghWat 3144.564 kb on + strand, within yghWat 3144.659 kb on - strand, within yghWat 3144.701 kb on - strand, within yghWat 3144.845 kb on + strandat 3144.854 kb on - strandat 3144.854 kb on - strandat 3144.911 kb on + strandat 3144.992 kb on + strand, within yghXat 3144.992 kb on + strand, within yghXat 3145.000 kb on - strand, within yghXat 3145.000 kb on - strand, within yghXat 3145.009 kb on + strand, within yghXat 3145.010 kb on - strand, within yghXat 3145.034 kb on + strand, within yghXat 3145.042 kb on - strand, within yghXat 3145.042 kb on - strand, within yghXat 3145.042 kb on - strand, within yghXat 3145.042 kb on - strand, within yghXat 3145.052 kb on - strand, within yghXat 3145.080 kb on + strand, within yghXat 3145.085 kb on + strand, within yghXat 3145.152 kb on + strand, within yghXat 3145.157 kb on + strand, within yghXat 3145.240 kb on + strand, within yghX

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + ciprofloxacin Time24hr
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3,142,271 - +0.4
3,142,284 - hybA b2996 0.11 +0.1
3,142,284 - hybA b2996 0.11 +0.9
3,142,284 - hybA b2996 0.11 +0.2
3,142,286 - hybA b2996 0.11 +0.8
3,142,286 - hybA b2996 0.11 +0.0
3,142,368 + hybA b2996 0.19 +0.5
3,142,410 + hybA b2996 0.24 +0.3
3,142,446 - hybA b2996 0.27 +0.4
3,142,585 - hybA b2996 0.41 +0.4
3,142,585 - hybA b2996 0.41 +0.6
3,142,587 + hybA b2996 0.42 +0.5
3,142,600 + hybA b2996 0.43 +0.2
3,142,676 - hybA b2996 0.51 +0.3
3,142,719 - hybA b2996 0.55 +0.5
3,142,719 - hybA b2996 0.55 +0.2
3,142,865 + hybA b2996 0.70 +0.2
3,142,877 + hybA b2996 0.71 +0.3
3,142,885 - hybA b2996 0.72 +0.4
3,142,995 - hybA b2996 0.83 +0.4
3,142,995 - hybA b2996 0.83 -0.1
3,143,008 + hybA b2996 0.84 +0.5
3,143,108 - +0.4
3,143,108 - +1.2
3,143,127 + +0.5
3,143,127 + -0.0
3,143,223 + +0.7
3,143,230 + +0.1
3,143,230 + +0.2
3,143,277 - hybO b2997 0.10 +1.3
3,143,279 - hybO b2997 0.10 +0.1
3,143,410 - hybO b2997 0.22 +0.5
3,143,410 - hybO b2997 0.22 +0.4
3,143,456 + hybO b2997 0.26 +0.3
3,143,471 - hybO b2997 0.27 +0.4
3,143,475 + hybO b2997 0.28 +0.2
3,143,499 - hybO b2997 0.30 +0.6
3,143,515 - hybO b2997 0.31 +0.4
3,143,521 - hybO b2997 0.32 +0.6
3,143,656 + hybO b2997 0.44 +0.3
3,143,677 - +0.3
3,143,677 - hybO b2997 0.46 -0.2
3,143,799 - hybO b2997 0.57 +0.3
3,143,861 - hybO b2997 0.62 +0.6
3,143,897 + hybO b2997 0.65 +0.2
3,143,960 - hybO b2997 0.71 +0.7
3,143,995 + hybO b2997 0.74 +0.5
3,144,007 + hybO b2997 0.75 +0.4
3,144,091 + hybO b2997 0.83 +0.5
3,144,105 + hybO b2997 0.84 -0.2
3,144,105 + hybO b2997 0.84 -0.1
3,144,201 + -0.3
3,144,202 - +0.0
3,144,250 + +0.4
3,144,259 + +0.4
3,144,259 + +0.0
3,144,267 + -0.1
3,144,269 + +0.4
3,144,269 + +0.0
3,144,395 + +0.6
3,144,395 + +0.3
3,144,398 - +0.4
3,144,437 - -0.1
3,144,485 + +0.1
3,144,553 + yghW b2998 0.28 +0.2
3,144,553 + yghW b2998 0.28 +0.0
3,144,553 + yghW b2998 0.28 +0.4
3,144,553 + yghW b2998 0.28 +0.7
3,144,553 + yghW b2998 0.28 +0.4
3,144,560 - yghW b2998 0.31 -0.2
3,144,561 - yghW b2998 0.31 +0.4
3,144,564 + yghW b2998 0.32 -0.0
3,144,564 + yghW b2998 0.32 -0.0
3,144,659 - yghW b2998 0.65 -0.3
3,144,701 - yghW b2998 0.80 +0.2
3,144,845 + +0.2
3,144,854 - +0.8
3,144,854 - +0.1
3,144,911 + -0.1
3,144,992 + yghX b4658 0.14 +0.1
3,144,992 + yghX b4658 0.14 +0.6
3,145,000 - yghX b4658 0.15 +0.4
3,145,000 - yghX b4658 0.15 +0.1
3,145,009 + yghX b4658 0.16 -1.3
3,145,010 - yghX b4658 0.16 -0.1
3,145,034 + yghX b4658 0.19 +0.6
3,145,042 - yghX b4658 0.20 -0.1
3,145,042 - yghX b4658 0.20 -0.2
3,145,042 - yghX b4658 0.20 -0.2
3,145,042 - yghX b4658 0.20 +0.3
3,145,052 - yghX b4658 0.21 +0.2
3,145,080 + yghX b4658 0.24 -0.4
3,145,085 + yghX b4658 0.25 +0.6
3,145,152 + yghX b4658 0.33 +0.1
3,145,157 + yghX b4658 0.33 -0.1
3,145,240 + yghX b4658 0.43 +0.4

Or see this region's nucleotide sequence