Experiment: LB + ciprofloxacin Time24hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ygfB and zapA are separated by 167 nucleotides zapA and ssrS are separated by 41 nucleotides ssrS and ygfA are separated by 75 nucleotides ygfA and rygC are separated by 59 nucleotides rygC and ibsC overlap by 99 nucleotides ibsC and serA are separated by 228 nucleotides
b2909: ygfB - orf, hypothetical protein (VIMSS), at 3,052,888 to 3,053,466
ygfB
b2910: zapA - protein that localizes to the cytokinetic ring (NCBI), at 3,053,634 to 3,053,963
zapA
b2911: ssrS - 6S regulatory RNA (NCBI), at 3,054,005 to 3,054,187
ssrS
b2912: ygfA - putative ligase (VIMSS), at 3,054,263 to 3,054,811
ygfA
b4446: rygC - Novel sRNA, function unknown; paralogous to the other QUAD sRNA genes (RefSeq), at 3,054,871 to 3,055,010
rygC
b4665: ibsC - toxic membrane protein (RefSeq), at 3,054,912 to 3,054,971
ibsC
b2913: serA - D-3-phosphoglycerate dehydrogenase (NCBI), at 3,055,200 to 3,056,432
serA
Position (kb)
3054
3055 Strain fitness (log2 ratio)
-2
-1
0
1 at 3053.263 kb on - strand, within ygfB at 3053.277 kb on + strand, within ygfB at 3053.308 kb on - strand, within ygfB at 3053.308 kb on - strand, within ygfB at 3053.355 kb on + strand, within ygfB at 3053.355 kb on + strand, within ygfB at 3053.359 kb on - strand, within ygfB at 3053.359 kb on - strand, within ygfB at 3053.392 kb on + strand, within ygfB at 3053.424 kb on + strand at 3053.434 kb on + strand at 3053.457 kb on + strand at 3053.457 kb on + strand at 3053.457 kb on + strand at 3053.457 kb on + strand at 3053.591 kb on + strand at 3053.591 kb on + strand at 3053.635 kb on + strand at 3053.635 kb on + strand at 3053.635 kb on + strand at 3053.679 kb on + strand, within zapA at 3053.722 kb on - strand, within zapA at 3053.731 kb on + strand, within zapA at 3053.886 kb on - strand, within zapA at 3053.886 kb on - strand, within zapA at 3053.891 kb on + strand, within zapA at 3053.992 kb on - strand at 3054.147 kb on + strand, within ssrS at 3054.147 kb on + strand, within ssrS at 3054.348 kb on + strand, within ygfA at 3054.388 kb on - strand, within ygfA at 3054.480 kb on - strand, within ygfA at 3054.480 kb on - strand, within ygfA at 3054.524 kb on + strand, within ygfA at 3054.532 kb on - strand, within ygfA at 3054.578 kb on + strand, within ygfA at 3054.696 kb on - strand, within ygfA at 3054.704 kb on + strand, within ygfA at 3054.704 kb on + strand, within ygfA at 3054.711 kb on + strand, within ygfA at 3054.711 kb on + strand, within ygfA at 3054.788 kb on - strand at 3054.897 kb on + strand, within rygC at 3054.946 kb on - strand at 3054.946 kb on - strand at 3055.023 kb on - strand at 3055.023 kb on - strand at 3055.280 kb on - strand at 3055.352 kb on + strand, within serA at 3055.352 kb on + strand, within serA at 3055.437 kb on - strand, within serA at 3055.535 kb on - strand, within serA at 3055.611 kb on + strand, within serA at 3055.611 kb on + strand, within serA at 3055.642 kb on - strand, within serA at 3055.674 kb on - strand, within serA at 3055.678 kb on + strand, within serA at 3055.678 kb on + strand, within serA at 3055.678 kb on + strand, within serA at 3055.708 kb on + strand, within serA at 3055.745 kb on + strand, within serA at 3055.745 kb on + strand, within serA at 3055.749 kb on - strand, within serA at 3055.749 kb on - strand, within serA at 3055.802 kb on - strand, within serA
Per-strain Table
Position Strand Gene LocusTag Fraction LB + ciprofloxacin Time24hr remove 3,053,263 - ygfB b2909 0.65 +0.4 3,053,277 + ygfB b2909 0.67 -0.1 3,053,308 - ygfB b2909 0.73 -0.6 3,053,308 - ygfB b2909 0.73 +0.4 3,053,355 + ygfB b2909 0.81 -0.0 3,053,355 + ygfB b2909 0.81 +0.4 3,053,359 - ygfB b2909 0.81 +0.5 3,053,359 - ygfB b2909 0.81 -0.1 3,053,392 + ygfB b2909 0.87 +0.3 3,053,424 + -0.1 3,053,434 + -0.2 3,053,457 + +0.3 3,053,457 + -0.1 3,053,457 + -0.1 3,053,457 + +0.3 3,053,591 + +0.4 3,053,591 + +0.6 3,053,635 + -0.9 3,053,635 + -0.0 3,053,635 + +0.4 3,053,679 + zapA b2910 0.14 -0.0 3,053,722 - zapA b2910 0.27 +0.2 3,053,731 + zapA b2910 0.29 -0.0 3,053,886 - zapA b2910 0.76 +0.0 3,053,886 - zapA b2910 0.76 +0.2 3,053,891 + zapA b2910 0.78 +0.3 3,053,992 - -0.1 3,054,147 + ssrS b2911 0.78 +0.1 3,054,147 + ssrS b2911 0.78 -0.1 3,054,348 + ygfA b2912 0.15 -0.4 3,054,388 - ygfA b2912 0.23 -1.4 3,054,480 - ygfA b2912 0.40 -2.1 3,054,480 - ygfA b2912 0.40 -0.8 3,054,524 + ygfA b2912 0.48 -0.0 3,054,532 - ygfA b2912 0.49 +0.6 3,054,578 + ygfA b2912 0.57 +1.6 3,054,696 - ygfA b2912 0.79 -1.8 3,054,704 + ygfA b2912 0.80 -0.4 3,054,704 + ygfA b2912 0.80 -0.4 3,054,711 + ygfA b2912 0.82 +1.6 3,054,711 + ygfA b2912 0.82 -2.2 3,054,788 - -0.8 3,054,897 + rygC b4446 0.19 -0.3 3,054,946 - +0.0 3,054,946 - +0.0 3,055,023 - +0.6 3,055,023 - +0.6 3,055,280 - +0.1 3,055,352 + serA b2913 0.12 +1.2 3,055,352 + serA b2913 0.12 -0.4 3,055,437 - serA b2913 0.19 +0.1 3,055,535 - serA b2913 0.27 +0.2 3,055,611 + serA b2913 0.33 +0.3 3,055,611 + serA b2913 0.33 +0.7 3,055,642 - serA b2913 0.36 +0.7 3,055,674 - serA b2913 0.38 +0.1 3,055,678 + serA b2913 0.39 -0.2 3,055,678 + serA b2913 0.39 +0.6 3,055,678 + serA b2913 0.39 -0.1 3,055,708 + serA b2913 0.41 +0.4 3,055,745 + serA b2913 0.44 +0.5 3,055,745 + serA b2913 0.44 +0.7 3,055,749 - serA b2913 0.45 +0.3 3,055,749 - serA b2913 0.45 +0.1 3,055,802 - serA b2913 0.49 -0.1
Or see this region's nucleotide sequence