Strain Fitness in Escherichia coli BW25113 around b2778

Experiment: LB + ciprofloxacin Time24hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntygcF and yqcG are separated by 138 nucleotidesyqcG and ygcG are separated by 13 nucleotidesygcG and eno are separated by 59 nucleotides b2777: ygcF - hypothetical protein (NCBI), at 2,902,769 to 2,903,440 ygcF b4682: yqcG - expressed protein (RefSeq), at 2,903,579 to 2,903,719 yqcG b2778: ygcG - orf, hypothetical protein (VIMSS), at 2,903,733 to 2,904,605 ygcG b2779: eno - phosphopyruvate hydratase (NCBI), at 2,904,665 to 2,905,963 eno Position (kb) 2903 2904 2905Strain fitness (log2 ratio) -1 0 1 2at 2902.735 kb on - strandat 2902.746 kb on + strandat 2902.770 kb on - strandat 2902.770 kb on - strandat 2902.797 kb on - strandat 2902.817 kb on - strandat 2902.832 kb on - strandat 2902.832 kb on - strandat 2902.846 kb on - strand, within ygcFat 2902.852 kb on - strand, within ygcFat 2902.852 kb on - strand, within ygcFat 2902.859 kb on - strand, within ygcFat 2902.876 kb on + strand, within ygcFat 2902.882 kb on - strand, within ygcFat 2902.887 kb on + strand, within ygcFat 2902.887 kb on - strand, within ygcFat 2902.887 kb on - strand, within ygcFat 2902.887 kb on - strand, within ygcFat 2902.887 kb on - strand, within ygcFat 2902.917 kb on - strand, within ygcFat 2902.917 kb on - strand, within ygcFat 2902.950 kb on - strand, within ygcFat 2902.981 kb on + strand, within ygcFat 2902.990 kb on - strand, within ygcFat 2902.990 kb on - strand, within ygcFat 2903.002 kb on + strand, within ygcFat 2903.010 kb on - strand, within ygcFat 2903.010 kb on - strand, within ygcFat 2903.015 kb on + strand, within ygcFat 2903.034 kb on - strand, within ygcFat 2903.050 kb on - strand, within ygcFat 2903.050 kb on - strand, within ygcFat 2903.058 kb on + strand, within ygcFat 2903.058 kb on + strand, within ygcFat 2903.074 kb on - strand, within ygcFat 2903.074 kb on - strand, within ygcFat 2903.187 kb on + strand, within ygcFat 2903.235 kb on + strand, within ygcFat 2903.307 kb on + strand, within ygcFat 2903.328 kb on + strand, within ygcFat 2903.332 kb on + strand, within ygcFat 2903.332 kb on + strand, within ygcFat 2903.335 kb on - strand, within ygcFat 2903.340 kb on - strand, within ygcFat 2903.388 kb on + strandat 2903.388 kb on + strandat 2903.394 kb on + strandat 2903.394 kb on + strandat 2903.396 kb on + strandat 2903.409 kb on + strandat 2903.416 kb on - strandat 2903.417 kb on - strandat 2903.417 kb on - strandat 2903.417 kb on - strandat 2903.437 kb on + strandat 2903.445 kb on - strandat 2903.448 kb on + strandat 2903.471 kb on - strandat 2903.503 kb on + strandat 2903.510 kb on + strandat 2903.525 kb on + strandat 2904.015 kb on - strand, within ygcGat 2904.015 kb on - strand, within ygcGat 2904.017 kb on + strand, within ygcGat 2904.017 kb on + strand, within ygcGat 2904.017 kb on - strand, within ygcGat 2904.027 kb on - strand, within ygcGat 2904.045 kb on + strand, within ygcGat 2904.061 kb on + strand, within ygcGat 2904.107 kb on + strand, within ygcGat 2904.113 kb on + strand, within ygcGat 2904.167 kb on + strand, within ygcGat 2904.205 kb on + strand, within ygcGat 2904.209 kb on + strand, within ygcGat 2904.439 kb on - strand, within ygcGat 2904.485 kb on - strand, within ygcGat 2904.485 kb on - strand, within ygcGat 2904.485 kb on - strand, within ygcGat 2904.492 kb on + strand, within ygcGat 2904.503 kb on - strand, within ygcGat 2904.503 kb on - strand, within ygcGat 2904.513 kb on + strand, within ygcGat 2904.525 kb on + strandat 2904.528 kb on + strandat 2904.573 kb on + strandat 2904.603 kb on - strandat 2904.665 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + ciprofloxacin Time24hr
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2,902,735 - +0.5
2,902,746 + +0.5
2,902,770 - +0.6
2,902,770 - +0.4
2,902,797 - -0.0
2,902,817 - +0.1
2,902,832 - -0.1
2,902,832 - +0.3
2,902,846 - ygcF b2777 0.11 +0.2
2,902,852 - ygcF b2777 0.12 -0.2
2,902,852 - ygcF b2777 0.12 +0.3
2,902,859 - ygcF b2777 0.13 +0.5
2,902,876 + ygcF b2777 0.16 +0.5
2,902,882 - ygcF b2777 0.17 -0.4
2,902,887 + ygcF b2777 0.18 -0.7
2,902,887 - ygcF b2777 0.18 +0.4
2,902,887 - ygcF b2777 0.18 +0.2
2,902,887 - ygcF b2777 0.18 +0.4
2,902,887 - ygcF b2777 0.18 +0.0
2,902,917 - ygcF b2777 0.22 +0.0
2,902,917 - ygcF b2777 0.22 +0.5
2,902,950 - ygcF b2777 0.27 +0.1
2,902,981 + ygcF b2777 0.32 +0.3
2,902,990 - ygcF b2777 0.33 +0.0
2,902,990 - ygcF b2777 0.33 -0.1
2,903,002 + ygcF b2777 0.35 -0.2
2,903,010 - ygcF b2777 0.36 +0.0
2,903,010 - ygcF b2777 0.36 +0.8
2,903,015 + ygcF b2777 0.37 +0.3
2,903,034 - ygcF b2777 0.39 -0.1
2,903,050 - ygcF b2777 0.42 +0.2
2,903,050 - ygcF b2777 0.42 +0.3
2,903,058 + ygcF b2777 0.43 +0.3
2,903,058 + ygcF b2777 0.43 -0.6
2,903,074 - ygcF b2777 0.45 -0.0
2,903,074 - ygcF b2777 0.45 +0.6
2,903,187 + ygcF b2777 0.62 -0.4
2,903,235 + ygcF b2777 0.69 +0.5
2,903,307 + ygcF b2777 0.80 +0.2
2,903,328 + ygcF b2777 0.83 -0.2
2,903,332 + ygcF b2777 0.84 -0.7
2,903,332 + ygcF b2777 0.84 -0.2
2,903,335 - ygcF b2777 0.84 +0.3
2,903,340 - ygcF b2777 0.85 +0.4
2,903,388 + +0.8
2,903,388 + +0.1
2,903,394 + +0.5
2,903,394 + +0.2
2,903,396 + -0.2
2,903,409 + -0.0
2,903,416 - +0.3
2,903,417 - -1.5
2,903,417 - +0.5
2,903,417 - +0.1
2,903,437 + -0.0
2,903,445 - -0.3
2,903,448 + -0.3
2,903,471 - -0.3
2,903,503 + -0.3
2,903,510 + +0.8
2,903,525 + -0.0
2,904,015 - ygcG b2778 0.32 +0.5
2,904,015 - ygcG b2778 0.32 +0.5
2,904,017 + ygcG b2778 0.33 +0.5
2,904,017 + ygcG b2778 0.33 +0.5
2,904,017 - ygcG b2778 0.33 +0.5
2,904,027 - ygcG b2778 0.34 +0.5
2,904,045 + ygcG b2778 0.36 +0.5
2,904,061 + ygcG b2778 0.38 +0.5
2,904,107 + ygcG b2778 0.43 +0.5
2,904,113 + ygcG b2778 0.44 +0.5
2,904,167 + ygcG b2778 0.50 +2.5
2,904,205 + ygcG b2778 0.54 +0.5
2,904,209 + ygcG b2778 0.55 +0.2
2,904,439 - ygcG b2778 0.81 +0.1
2,904,485 - ygcG b2778 0.86 +0.2
2,904,485 - ygcG b2778 0.86 +0.7
2,904,485 - ygcG b2778 0.86 +0.4
2,904,492 + ygcG b2778 0.87 +0.6
2,904,503 - ygcG b2778 0.88 +0.8
2,904,503 - ygcG b2778 0.88 +1.5
2,904,513 + ygcG b2778 0.89 +1.6
2,904,525 + +1.3
2,904,528 + +1.1
2,904,573 + +0.1
2,904,603 - +0.4
2,904,665 - +0.9

Or see this region's nucleotide sequence