Experiment: LB + ciprofloxacin Time24hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hyfD and hyfE are separated by 11 nucleotides hyfE and hyfF are separated by 4 nucleotides hyfF and hyfG overlap by 11 nucleotides
b2484: hyfD - hydrogenase 4 membrane subunit (NCBI), at 2,602,833 to 2,604,272
hyfD
b2485: hyfE - hydrogenase 4, membrane subunit (NCBI), at 2,604,284 to 2,604,934
hyfE
b2486: hyfF - NADH dehydrogenase subunit N (NCBI), at 2,604,939 to 2,606,519
hyfF
b2487: hyfG - hydrogenase 4, subunit (NCBI), at 2,606,509 to 2,608,176
hyfG
Position (kb)
2604
2605
2606
2607 Strain fitness (log2 ratio)
-1
0
1 at 2604.064 kb on - strand, within hyfD at 2604.064 kb on - strand, within hyfD at 2604.216 kb on + strand at 2604.216 kb on + strand at 2604.284 kb on - strand at 2604.284 kb on - strand at 2604.340 kb on + strand at 2604.488 kb on + strand, within hyfE at 2604.488 kb on + strand, within hyfE at 2604.513 kb on - strand, within hyfE at 2604.598 kb on - strand, within hyfE at 2604.626 kb on + strand, within hyfE at 2604.700 kb on + strand, within hyfE at 2604.708 kb on - strand, within hyfE at 2604.708 kb on - strand, within hyfE at 2604.828 kb on + strand, within hyfE at 2604.862 kb on - strand, within hyfE at 2604.882 kb on + strand at 2604.882 kb on + strand at 2605.055 kb on - strand at 2605.065 kb on - strand at 2605.065 kb on - strand at 2605.071 kb on - strand at 2605.140 kb on - strand, within hyfF at 2605.210 kb on + strand, within hyfF at 2605.256 kb on + strand, within hyfF at 2605.289 kb on - strand, within hyfF at 2605.374 kb on + strand, within hyfF at 2605.374 kb on + strand, within hyfF at 2605.374 kb on + strand, within hyfF at 2605.420 kb on - strand, within hyfF at 2605.439 kb on - strand, within hyfF at 2605.439 kb on - strand, within hyfF at 2605.478 kb on + strand, within hyfF at 2605.478 kb on + strand, within hyfF at 2605.478 kb on + strand, within hyfF at 2605.481 kb on + strand, within hyfF at 2605.521 kb on - strand, within hyfF at 2605.579 kb on + strand, within hyfF at 2605.601 kb on - strand, within hyfF at 2605.601 kb on - strand, within hyfF at 2605.686 kb on - strand, within hyfF at 2605.686 kb on - strand, within hyfF at 2605.829 kb on - strand, within hyfF at 2605.969 kb on + strand, within hyfF at 2605.969 kb on + strand, within hyfF at 2606.180 kb on + strand, within hyfF at 2606.240 kb on - strand, within hyfF at 2606.420 kb on - strand at 2606.450 kb on + strand at 2606.611 kb on - strand at 2606.648 kb on + strand at 2606.761 kb on - strand, within hyfG at 2606.893 kb on - strand, within hyfG at 2606.922 kb on + strand, within hyfG at 2606.922 kb on + strand, within hyfG at 2606.990 kb on + strand, within hyfG at 2606.998 kb on - strand, within hyfG at 2606.998 kb on - strand, within hyfG at 2607.008 kb on - strand, within hyfG at 2607.199 kb on + strand, within hyfG at 2607.250 kb on + strand, within hyfG at 2607.250 kb on + strand, within hyfG at 2607.258 kb on + strand, within hyfG at 2607.260 kb on + strand, within hyfG at 2607.264 kb on + strand, within hyfG at 2607.272 kb on - strand, within hyfG at 2607.354 kb on + strand, within hyfG at 2607.362 kb on + strand, within hyfG at 2607.397 kb on - strand, within hyfG
Per-strain Table
Position Strand Gene LocusTag Fraction LB + ciprofloxacin Time24hr remove 2,604,064 - hyfD b2484 0.85 +0.4 2,604,064 - hyfD b2484 0.85 +0.4 2,604,216 + +0.5 2,604,216 + -0.6 2,604,284 - +0.3 2,604,284 - +0.1 2,604,340 + -0.1 2,604,488 + hyfE b2485 0.31 +0.5 2,604,488 + hyfE b2485 0.31 +0.4 2,604,513 - hyfE b2485 0.35 -0.3 2,604,598 - hyfE b2485 0.48 -0.1 2,604,626 + hyfE b2485 0.53 -0.3 2,604,700 + hyfE b2485 0.64 +0.7 2,604,708 - hyfE b2485 0.65 +0.3 2,604,708 - hyfE b2485 0.65 +0.7 2,604,828 + hyfE b2485 0.84 +0.1 2,604,862 - hyfE b2485 0.89 -0.2 2,604,882 + -0.1 2,604,882 + +0.5 2,605,055 - -0.2 2,605,065 - +0.4 2,605,065 - +0.2 2,605,071 - +0.2 2,605,140 - hyfF b2486 0.13 -0.2 2,605,210 + hyfF b2486 0.17 +0.6 2,605,256 + hyfF b2486 0.20 +0.1 2,605,289 - hyfF b2486 0.22 +0.1 2,605,374 + hyfF b2486 0.28 +0.9 2,605,374 + hyfF b2486 0.28 +0.5 2,605,374 + hyfF b2486 0.28 +0.1 2,605,420 - hyfF b2486 0.30 +0.8 2,605,439 - hyfF b2486 0.32 -0.0 2,605,439 - hyfF b2486 0.32 +1.4 2,605,478 + hyfF b2486 0.34 +0.1 2,605,478 + hyfF b2486 0.34 -0.4 2,605,478 + hyfF b2486 0.34 +0.3 2,605,481 + hyfF b2486 0.34 +1.0 2,605,521 - hyfF b2486 0.37 +0.2 2,605,579 + hyfF b2486 0.40 -0.2 2,605,601 - hyfF b2486 0.42 +1.0 2,605,601 - hyfF b2486 0.42 -0.3 2,605,686 - hyfF b2486 0.47 +1.1 2,605,686 - hyfF b2486 0.47 +0.6 2,605,829 - hyfF b2486 0.56 +0.5 2,605,969 + hyfF b2486 0.65 +1.0 2,605,969 + hyfF b2486 0.65 +0.5 2,606,180 + hyfF b2486 0.78 +0.9 2,606,240 - hyfF b2486 0.82 -0.5 2,606,420 - +0.1 2,606,450 + +0.4 2,606,611 - +0.0 2,606,648 + +0.3 2,606,761 - hyfG b2487 0.15 +0.1 2,606,893 - hyfG b2487 0.23 +0.5 2,606,922 + hyfG b2487 0.25 +0.1 2,606,922 + hyfG b2487 0.25 +0.3 2,606,990 + hyfG b2487 0.29 +0.7 2,606,998 - hyfG b2487 0.29 -0.0 2,606,998 - hyfG b2487 0.29 +0.3 2,607,008 - hyfG b2487 0.30 +0.5 2,607,199 + hyfG b2487 0.41 +0.3 2,607,250 + hyfG b2487 0.44 +0.2 2,607,250 + hyfG b2487 0.44 +0.9 2,607,258 + hyfG b2487 0.45 -0.2 2,607,260 + hyfG b2487 0.45 +0.1 2,607,264 + hyfG b2487 0.45 +0.0 2,607,272 - hyfG b2487 0.46 +0.4 2,607,354 + hyfG b2487 0.51 -0.0 2,607,362 + hyfG b2487 0.51 -0.2 2,607,397 - hyfG b2487 0.53 +1.1
Or see this region's nucleotide sequence