Strain Fitness in Escherichia coli BW25113 around b1930

Experiment: LB + ciprofloxacin Time24hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyedE and yedF overlap by 4 nucleotidesyedF and yedK are separated by 108 nucleotidesyedK and yedL are separated by 110 nucleotides b1929: yedE - predicted inner membrane protein (NCBI), at 2,006,301 to 2,007,506 yedE b1930: yedF - hypothetical protein (NCBI), at 2,007,503 to 2,007,736 yedF b1931: yedK - hypothetical protein (NCBI), at 2,007,845 to 2,008,513 yedK b1932: yedL - predicted acyltransferase (NCBI), at 2,008,624 to 2,009,103 yedL Position (kb) 2007 2008Strain fitness (log2 ratio) -1 0 1at 2006.508 kb on + strand, within yedEat 2006.605 kb on - strand, within yedEat 2006.732 kb on - strand, within yedEat 2006.767 kb on - strand, within yedEat 2006.772 kb on - strand, within yedEat 2006.951 kb on - strand, within yedEat 2006.989 kb on + strand, within yedEat 2007.062 kb on + strand, within yedEat 2007.062 kb on + strand, within yedEat 2007.131 kb on + strand, within yedEat 2007.131 kb on + strand, within yedEat 2007.244 kb on - strand, within yedEat 2007.257 kb on - strand, within yedEat 2007.261 kb on + strand, within yedEat 2007.263 kb on - strand, within yedEat 2007.314 kb on + strand, within yedEat 2007.541 kb on + strand, within yedFat 2007.542 kb on + strand, within yedFat 2007.627 kb on + strand, within yedFat 2007.628 kb on - strand, within yedFat 2007.630 kb on + strand, within yedFat 2007.656 kb on - strand, within yedFat 2007.710 kb on + strand, within yedFat 2007.744 kb on + strandat 2007.763 kb on - strandat 2007.767 kb on - strandat 2007.775 kb on - strandat 2007.931 kb on + strand, within yedKat 2008.001 kb on - strand, within yedKat 2008.003 kb on + strand, within yedKat 2008.003 kb on + strand, within yedKat 2008.095 kb on + strand, within yedKat 2008.119 kb on - strand, within yedKat 2008.295 kb on - strand, within yedKat 2008.389 kb on + strand, within yedKat 2008.506 kb on + strandat 2008.506 kb on + strandat 2008.534 kb on + strandat 2008.661 kb on - strandat 2008.671 kb on - strandat 2008.696 kb on - strand, within yedLat 2008.707 kb on - strand, within yedLat 2008.707 kb on - strand, within yedLat 2008.728 kb on + strand, within yedLat 2008.729 kb on + strand, within yedL

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + ciprofloxacin Time24hr
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2,006,508 + yedE b1929 0.17 +0.6
2,006,605 - yedE b1929 0.25 +1.1
2,006,732 - yedE b1929 0.36 +0.1
2,006,767 - yedE b1929 0.39 +0.1
2,006,772 - yedE b1929 0.39 -0.3
2,006,951 - yedE b1929 0.54 +0.0
2,006,989 + yedE b1929 0.57 +0.8
2,007,062 + yedE b1929 0.63 +0.1
2,007,062 + yedE b1929 0.63 -0.1
2,007,131 + yedE b1929 0.69 -0.2
2,007,131 + yedE b1929 0.69 +0.3
2,007,244 - yedE b1929 0.78 -0.1
2,007,257 - yedE b1929 0.79 +0.3
2,007,261 + yedE b1929 0.80 +0.1
2,007,263 - yedE b1929 0.80 +0.1
2,007,314 + yedE b1929 0.84 -0.0
2,007,541 + yedF b1930 0.16 +0.7
2,007,542 + yedF b1930 0.17 +0.4
2,007,627 + yedF b1930 0.53 +0.4
2,007,628 - yedF b1930 0.53 +0.9
2,007,630 + yedF b1930 0.54 +0.3
2,007,656 - yedF b1930 0.65 +0.2
2,007,710 + yedF b1930 0.88 +0.2
2,007,744 + -0.1
2,007,763 - -0.2
2,007,767 - +0.4
2,007,775 - -0.5
2,007,931 + yedK b1931 0.13 -0.2
2,008,001 - yedK b1931 0.23 -0.0
2,008,003 + yedK b1931 0.24 +0.1
2,008,003 + yedK b1931 0.24 -0.4
2,008,095 + yedK b1931 0.37 -0.1
2,008,119 - yedK b1931 0.41 +0.2
2,008,295 - yedK b1931 0.67 +0.3
2,008,389 + yedK b1931 0.81 +0.3
2,008,506 + -1.1
2,008,506 + +0.3
2,008,534 + +0.1
2,008,661 - -0.9
2,008,671 - +0.2
2,008,696 - yedL b1932 0.15 +0.9
2,008,707 - yedL b1932 0.17 +0.4
2,008,707 - yedL b1932 0.17 +0.6
2,008,728 + yedL b1932 0.22 +0.6
2,008,729 + yedL b1932 0.22 -0.6

Or see this region's nucleotide sequence