Strain Fitness in Escherichia coli BW25113 around b4686

Experiment: LB + ciprofloxacin Time8hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthemN and yshB are separated by 228 nucleotidesyshB and glnG are separated by 111 nucleotides b3867: hemN - O2-independent coproporphyrinogen III oxidase (VIMSS), at 4,050,068 to 4,051,441 hemN b4686: yshB - expressed protein (RefSeq), at 4,051,670 to 4,051,780 yshB b3868: glnG - fused DNA-binding response regulator in two-component regulatory system with GlnL: response regulator/sigma54 interaction protein (NCBI), at 4,051,892 to 4,053,301 glnG Position (kb) 4051 4052Strain fitness (log2 ratio) -2 -1 0 1at 4050.674 kb on - strand, within hemNat 4050.683 kb on + strand, within hemNat 4050.683 kb on + strand, within hemNat 4050.742 kb on + strand, within hemNat 4050.782 kb on + strand, within hemNat 4050.783 kb on - strand, within hemNat 4050.783 kb on - strand, within hemNat 4050.783 kb on - strand, within hemNat 4050.988 kb on - strand, within hemNat 4050.998 kb on - strand, within hemNat 4051.005 kb on + strand, within hemNat 4051.005 kb on + strand, within hemNat 4051.008 kb on - strand, within hemNat 4051.018 kb on - strand, within hemNat 4051.030 kb on + strand, within hemNat 4051.043 kb on - strand, within hemNat 4051.043 kb on - strand, within hemNat 4051.069 kb on + strand, within hemNat 4051.081 kb on + strand, within hemNat 4051.089 kb on - strand, within hemNat 4051.089 kb on - strand, within hemNat 4051.091 kb on - strand, within hemNat 4051.107 kb on + strand, within hemNat 4051.133 kb on + strand, within hemNat 4051.154 kb on - strand, within hemNat 4051.154 kb on - strand, within hemNat 4051.185 kb on + strand, within hemNat 4051.185 kb on + strand, within hemNat 4051.216 kb on + strand, within hemNat 4051.224 kb on - strand, within hemNat 4051.229 kb on + strand, within hemNat 4051.229 kb on + strand, within hemNat 4051.275 kb on + strand, within hemNat 4051.418 kb on + strandat 4051.418 kb on + strandat 4051.499 kb on + strandat 4051.613 kb on - strandat 4051.613 kb on - strandat 4051.628 kb on - strandat 4051.679 kb on - strandat 4051.679 kb on - strandat 4051.692 kb on + strand, within yshBat 4051.726 kb on + strand, within yshBat 4051.726 kb on + strand, within yshBat 4051.730 kb on + strand, within yshBat 4051.741 kb on - strand, within yshBat 4051.808 kb on + strandat 4051.894 kb on + strandat 4051.894 kb on + strandat 4051.909 kb on + strandat 4051.909 kb on + strandat 4051.909 kb on - strandat 4051.929 kb on + strandat 4051.941 kb on + strandat 4052.012 kb on + strandat 4052.015 kb on - strandat 4052.126 kb on + strand, within glnGat 4052.126 kb on + strand, within glnGat 4052.197 kb on - strand, within glnGat 4052.255 kb on + strand, within glnGat 4052.328 kb on - strand, within glnGat 4052.423 kb on + strand, within glnGat 4052.423 kb on + strand, within glnGat 4052.497 kb on + strand, within glnGat 4052.497 kb on + strand, within glnGat 4052.614 kb on + strand, within glnGat 4052.688 kb on + strand, within glnGat 4052.712 kb on + strand, within glnGat 4052.769 kb on - strand, within glnG

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + ciprofloxacin Time8hr
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4,050,674 - hemN b3867 0.44 +0.5
4,050,683 + hemN b3867 0.45 -0.7
4,050,683 + hemN b3867 0.45 -0.0
4,050,742 + hemN b3867 0.49 -0.1
4,050,782 + hemN b3867 0.52 +0.5
4,050,783 - hemN b3867 0.52 +0.5
4,050,783 - hemN b3867 0.52 +0.2
4,050,783 - hemN b3867 0.52 -0.5
4,050,988 - hemN b3867 0.67 +0.1
4,050,998 - hemN b3867 0.68 +0.1
4,051,005 + hemN b3867 0.68 -0.7
4,051,005 + hemN b3867 0.68 -0.1
4,051,008 - hemN b3867 0.68 +0.5
4,051,018 - hemN b3867 0.69 +0.4
4,051,030 + hemN b3867 0.70 -0.2
4,051,043 - hemN b3867 0.71 +0.2
4,051,043 - hemN b3867 0.71 +0.4
4,051,069 + hemN b3867 0.73 -0.2
4,051,081 + hemN b3867 0.74 -1.1
4,051,089 - hemN b3867 0.74 +0.7
4,051,089 - hemN b3867 0.74 -0.3
4,051,091 - hemN b3867 0.74 +0.0
4,051,107 + hemN b3867 0.76 -0.2
4,051,133 + hemN b3867 0.78 +1.2
4,051,154 - hemN b3867 0.79 +0.7
4,051,154 - hemN b3867 0.79 +0.5
4,051,185 + hemN b3867 0.81 -0.5
4,051,185 + hemN b3867 0.81 +0.7
4,051,216 + hemN b3867 0.84 -0.9
4,051,224 - hemN b3867 0.84 -0.2
4,051,229 + hemN b3867 0.84 +0.1
4,051,229 + hemN b3867 0.84 -1.6
4,051,275 + hemN b3867 0.88 +0.3
4,051,418 + +0.1
4,051,418 + +0.3
4,051,499 + +0.3
4,051,613 - +0.4
4,051,613 - +0.0
4,051,628 - -0.3
4,051,679 - -0.0
4,051,679 - +0.3
4,051,692 + yshB b4686 0.20 +0.1
4,051,726 + yshB b4686 0.50 +1.6
4,051,726 + yshB b4686 0.50 +0.3
4,051,730 + yshB b4686 0.54 +0.4
4,051,741 - yshB b4686 0.64 -0.0
4,051,808 + -0.3
4,051,894 + +0.3
4,051,894 + +0.2
4,051,909 + -1.2
4,051,909 + -1.8
4,051,909 - -0.6
4,051,929 + +0.3
4,051,941 + -0.4
4,052,012 + -0.0
4,052,015 - -1.2
4,052,126 + glnG b3868 0.17 -0.8
4,052,126 + glnG b3868 0.17 -0.8
4,052,197 - glnG b3868 0.22 -1.0
4,052,255 + glnG b3868 0.26 -0.3
4,052,328 - glnG b3868 0.31 -1.2
4,052,423 + glnG b3868 0.38 -1.7
4,052,423 + glnG b3868 0.38 -1.2
4,052,497 + glnG b3868 0.43 -0.8
4,052,497 + glnG b3868 0.43 -0.4
4,052,614 + glnG b3868 0.51 -1.0
4,052,688 + glnG b3868 0.56 -0.5
4,052,712 + glnG b3868 0.58 -0.5
4,052,769 - glnG b3868 0.62 -1.0

Or see this region's nucleotide sequence