Experiment: LB + ciprofloxacin Time8hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt baeR and b2080 are separated by 190 nucleotides b2080 and yegQ are separated by 146 nucleotides yegQ and cyaR are separated by 84 nucleotides cyaR and ogrK are separated by 101 nucleotides ogrK and yegZ are separated by 81 nucleotides yegZ and b2085 are separated by 174 nucleotides
b2079: baeR - DNA-binding response regulator in two-component regulatory system with BaeS (NCBI), at 2,162,300 to 2,163,022
baeR
b2080: b2080 - orf, hypothetical protein (VIMSS), at 2,163,213 to 2,163,545
b2080
b2081: yegQ - predicted peptidase (NCBI), at 2,163,692 to 2,165,053
yegQ
b4438: cyaR - sRNA effector of ompX mRNA instability, cAMP-induced; hfq-dependent (RefSeq), at 2,165,138 to 2,165,224
cyaR
b2082: ogrK - DNA-binding transcriptional regulator prophage P2 remnant (NCBI), at 2,165,326 to 2,165,544
ogrK
b2083: yegZ - orf, hypothetical protein (VIMSS), at 2,165,626 to 2,165,838
yegZ
b2085: b2085 - orf, hypothetical protein (VIMSS), at 2,166,013 to 2,166,330
b2085
Position (kb)
2163
2164
2165
2166 Strain fitness (log2 ratio)
-1
0
1 at 2162.817 kb on + strand, within baeR at 2162.817 kb on + strand, within baeR at 2162.863 kb on + strand, within baeR at 2162.997 kb on - strand at 2163.076 kb on + strand at 2163.084 kb on - strand at 2163.084 kb on - strand at 2163.217 kb on - strand at 2163.246 kb on + strand at 2163.350 kb on + strand, within b2080 at 2163.536 kb on - strand at 2163.571 kb on - strand at 2163.571 kb on - strand at 2163.599 kb on - strand at 2163.599 kb on - strand at 2163.635 kb on + strand at 2163.725 kb on + strand at 2163.733 kb on + strand at 2163.765 kb on + strand at 2163.851 kb on - strand, within yegQ at 2163.901 kb on + strand, within yegQ at 2163.902 kb on + strand, within yegQ at 2164.134 kb on + strand, within yegQ at 2164.277 kb on - strand, within yegQ at 2164.277 kb on - strand, within yegQ at 2164.449 kb on - strand, within yegQ at 2164.538 kb on - strand, within yegQ at 2164.578 kb on + strand, within yegQ at 2164.578 kb on + strand, within yegQ at 2164.600 kb on + strand, within yegQ at 2164.614 kb on - strand, within yegQ at 2164.614 kb on - strand, within yegQ at 2164.653 kb on + strand, within yegQ at 2164.657 kb on + strand, within yegQ at 2164.713 kb on + strand, within yegQ at 2164.713 kb on + strand, within yegQ at 2164.798 kb on + strand, within yegQ at 2164.927 kb on - strand at 2164.950 kb on + strand at 2165.031 kb on + strand at 2165.109 kb on - strand at 2165.109 kb on - strand at 2165.177 kb on - strand, within cyaR at 2165.184 kb on + strand, within cyaR at 2165.184 kb on + strand, within cyaR at 2165.383 kb on - strand, within ogrK at 2165.423 kb on - strand, within ogrK at 2165.658 kb on - strand, within yegZ at 2165.732 kb on + strand, within yegZ at 2165.732 kb on + strand, within yegZ at 2165.872 kb on + strand at 2165.894 kb on - strand at 2165.907 kb on + strand at 2165.929 kb on + strand at 2165.929 kb on + strand at 2165.964 kb on + strand at 2165.968 kb on + strand at 2166.018 kb on + strand at 2166.018 kb on + strand at 2166.023 kb on + strand at 2166.047 kb on + strand, within b2085 at 2166.047 kb on + strand, within b2085 at 2166.047 kb on - strand, within b2085 at 2166.047 kb on - strand, within b2085 at 2166.052 kb on - strand, within b2085
Per-strain Table
Position Strand Gene LocusTag Fraction LB + ciprofloxacin Time8hr remove 2,162,817 + baeR b2079 0.72 -0.5 2,162,817 + baeR b2079 0.72 -0.0 2,162,863 + baeR b2079 0.78 -0.2 2,162,997 - +0.3 2,163,076 + +0.2 2,163,084 - +0.1 2,163,084 - -0.0 2,163,217 - +0.3 2,163,246 + -0.1 2,163,350 + b2080 b2080 0.41 -0.1 2,163,536 - +0.2 2,163,571 - -0.0 2,163,571 - -0.2 2,163,599 - -0.3 2,163,599 - +0.1 2,163,635 + -0.5 2,163,725 + +1.0 2,163,733 + -0.1 2,163,765 + -0.4 2,163,851 - yegQ b2081 0.12 -0.3 2,163,901 + yegQ b2081 0.15 -0.2 2,163,902 + yegQ b2081 0.15 -0.5 2,164,134 + yegQ b2081 0.32 -0.3 2,164,277 - yegQ b2081 0.43 -0.0 2,164,277 - yegQ b2081 0.43 -0.7 2,164,449 - yegQ b2081 0.56 -0.6 2,164,538 - yegQ b2081 0.62 +0.0 2,164,578 + yegQ b2081 0.65 -0.5 2,164,578 + yegQ b2081 0.65 -0.7 2,164,600 + yegQ b2081 0.67 +0.1 2,164,614 - yegQ b2081 0.68 -1.5 2,164,614 - yegQ b2081 0.68 -0.4 2,164,653 + yegQ b2081 0.71 -0.6 2,164,657 + yegQ b2081 0.71 -0.5 2,164,713 + yegQ b2081 0.75 -0.5 2,164,713 + yegQ b2081 0.75 -1.0 2,164,798 + yegQ b2081 0.81 -0.5 2,164,927 - -0.1 2,164,950 + -0.5 2,165,031 + +0.5 2,165,109 - -0.0 2,165,109 - +1.3 2,165,177 - cyaR b4438 0.45 -0.0 2,165,184 + cyaR b4438 0.53 +0.1 2,165,184 + cyaR b4438 0.53 +0.7 2,165,383 - ogrK b2082 0.26 +0.5 2,165,423 - ogrK b2082 0.44 +0.3 2,165,658 - yegZ b2083 0.15 +0.3 2,165,732 + yegZ b2083 0.50 -0.3 2,165,732 + yegZ b2083 0.50 +0.4 2,165,872 + +0.2 2,165,894 - +0.6 2,165,907 + +0.3 2,165,929 + -0.8 2,165,929 + -1.4 2,165,964 + +0.4 2,165,968 + +1.0 2,166,018 + -1.3 2,166,018 + -0.3 2,166,023 + +1.4 2,166,047 + b2085 b2085 0.11 -0.0 2,166,047 + b2085 b2085 0.11 -0.2 2,166,047 - b2085 b2085 0.11 +0.4 2,166,047 - b2085 b2085 0.11 -1.6 2,166,052 - b2085 b2085 0.12 +0.2
Or see this region's nucleotide sequence