Strain Fitness in Escherichia coli BW25113 around b2051

Experiment: LB + ciprofloxacin Time8hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntwcaI and wcaH overlap by 4 nucleotideswcaH and fcl are separated by 2 nucleotidesfcl and gmd are separated by 2 nucleotides b2050: wcaI - predicted glycosyl transferase (NCBI), at 2,122,547 to 2,123,770 wcaI b2051: wcaH - GDP-mannose mannosyl hydrolase (VIMSS), at 2,123,767 to 2,124,246 wcaH b2052: fcl - bifunctional GDP-fucose synthetase: GDP-4-dehydro-6-deoxy-D-mannose epimerase/ GDP-4-dehydro-6-L-deoxygalactose reductase (NCBI), at 2,124,249 to 2,125,214 fcl b2053: gmd - GDP-D-mannose dehydratase, NAD(P)-binding (NCBI), at 2,125,217 to 2,126,338 gmd Position (kb) 2123 2124 2125Strain fitness (log2 ratio) -1 0 1at 2123.290 kb on + strand, within wcaIat 2123.290 kb on + strand, within wcaIat 2123.479 kb on + strand, within wcaIat 2123.610 kb on + strand, within wcaIat 2123.668 kb on + strandat 2123.822 kb on + strand, within wcaHat 2124.177 kb on - strand, within wcaHat 2124.282 kb on + strandat 2124.282 kb on + strandat 2124.465 kb on + strand, within fclat 2124.601 kb on + strand, within fclat 2124.630 kb on + strand, within fclat 2124.630 kb on + strand, within fclat 2124.722 kb on - strand, within fclat 2124.813 kb on - strand, within fclat 2124.848 kb on - strand, within fclat 2124.896 kb on + strand, within fclat 2124.951 kb on + strand, within fclat 2124.951 kb on + strand, within fclat 2125.046 kb on - strand, within fclat 2125.113 kb on + strand, within fclat 2125.189 kb on + strandat 2125.221 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + ciprofloxacin Time8hr
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2,123,290 + wcaI b2050 0.61 -0.2
2,123,290 + wcaI b2050 0.61 -0.3
2,123,479 + wcaI b2050 0.76 +0.2
2,123,610 + wcaI b2050 0.87 +0.3
2,123,668 + +0.2
2,123,822 + wcaH b2051 0.11 +0.0
2,124,177 - wcaH b2051 0.85 +1.1
2,124,282 + -0.1
2,124,282 + +0.2
2,124,465 + fcl b2052 0.22 -0.1
2,124,601 + fcl b2052 0.36 +0.1
2,124,630 + fcl b2052 0.39 -0.6
2,124,630 + fcl b2052 0.39 -0.5
2,124,722 - fcl b2052 0.49 +0.5
2,124,813 - fcl b2052 0.58 +0.6
2,124,848 - fcl b2052 0.62 +0.6
2,124,896 + fcl b2052 0.67 +0.3
2,124,951 + fcl b2052 0.73 +0.3
2,124,951 + fcl b2052 0.73 +0.4
2,125,046 - fcl b2052 0.83 +0.4
2,125,113 + fcl b2052 0.89 -0.1
2,125,189 + +0.4
2,125,221 - +0.0

Or see this region's nucleotide sequence