Strain Fitness in Escherichia coli BW25113 around b1850

Experiment: LB + ciprofloxacin Time8hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpurT and eda are separated by 55 nucleotideseda and edd are separated by 36 nucleotides b1849: purT - phosphoribosylglycinamide formyltransferase 2 (NCBI), at 1,928,905 to 1,930,083 purT b1850: eda - keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase (NCBI), at 1,930,139 to 1,930,780 eda b1851: edd - phosphogluconate dehydratase (NCBI), at 1,930,817 to 1,932,628 edd Position (kb) 1930 1931Strain fitness (log2 ratio) -2 -1 0 1at 1929.150 kb on - strand, within purTat 1929.362 kb on + strand, within purTat 1929.362 kb on + strand, within purTat 1929.640 kb on - strand, within purTat 1929.640 kb on - strand, within purTat 1929.683 kb on + strand, within purTat 1929.683 kb on + strand, within purTat 1929.759 kb on - strand, within purTat 1929.800 kb on + strand, within purTat 1930.003 kb on + strandat 1930.263 kb on + strand, within edaat 1930.411 kb on - strand, within edaat 1930.626 kb on - strand, within edaat 1930.626 kb on - strand, within edaat 1930.833 kb on + strandat 1930.841 kb on - strandat 1930.843 kb on + strandat 1930.843 kb on + strandat 1930.988 kb on + strandat 1931.044 kb on - strand, within eddat 1931.065 kb on - strand, within eddat 1931.065 kb on - strand, within eddat 1931.095 kb on + strand, within eddat 1931.142 kb on + strand, within eddat 1931.353 kb on - strand, within eddat 1931.392 kb on + strand, within eddat 1931.484 kb on - strand, within eddat 1931.549 kb on - strand, within eddat 1931.549 kb on - strand, within eddat 1931.566 kb on - strand, within eddat 1931.566 kb on - strand, within eddat 1931.591 kb on - strand, within edd

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + ciprofloxacin Time8hr
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1,929,150 - purT b1849 0.21 +0.7
1,929,362 + purT b1849 0.39 +0.8
1,929,362 + purT b1849 0.39 +0.0
1,929,640 - purT b1849 0.62 +0.7
1,929,640 - purT b1849 0.62 +0.5
1,929,683 + purT b1849 0.66 +0.2
1,929,683 + purT b1849 0.66 -0.0
1,929,759 - purT b1849 0.72 +0.1
1,929,800 + purT b1849 0.76 +0.1
1,930,003 + +0.8
1,930,263 + eda b1850 0.19 -1.6
1,930,411 - eda b1850 0.42 -0.9
1,930,626 - eda b1850 0.76 -1.7
1,930,626 - eda b1850 0.76 -2.5
1,930,833 + +0.2
1,930,841 - -0.2
1,930,843 + +0.2
1,930,843 + -0.9
1,930,988 + +0.3
1,931,044 - edd b1851 0.13 -0.1
1,931,065 - edd b1851 0.14 +0.2
1,931,065 - edd b1851 0.14 -0.4
1,931,095 + edd b1851 0.15 +0.0
1,931,142 + edd b1851 0.18 +0.1
1,931,353 - edd b1851 0.30 +0.3
1,931,392 + edd b1851 0.32 -0.4
1,931,484 - edd b1851 0.37 +0.2
1,931,549 - edd b1851 0.40 +0.6
1,931,549 - edd b1851 0.40 +0.8
1,931,566 - edd b1851 0.41 +0.2
1,931,566 - edd b1851 0.41 +0.5
1,931,591 - edd b1851 0.43 -0.1

Or see this region's nucleotide sequence