Strain Fitness in Escherichia coli BW25113 around b1830

Experiment: LB + ciprofloxacin Time8hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthtpX and prc are separated by 191 nucleotidesprc and proQ are separated by 19 nucleotidesproQ and yebR are separated by 96 nucleotides b1829: htpX - heat shock protein HtpX (NCBI), at 1,909,719 to 1,910,600 htpX b1830: prc - carboxy-terminal protease for penicillin-binding protein 3 (NCBI), at 1,910,792 to 1,912,840 prc b1831: proQ - putative solute/DNA competence effector (NCBI), at 1,912,860 to 1,913,558 proQ b1832: yebR - free methionine-(R)-sulfoxide reductase (RefSeq), at 1,913,655 to 1,914,152 yebR Position (kb) 1910 1911 1912 1913Strain fitness (log2 ratio) -1 0 1 2at 1909.831 kb on - strand, within htpXat 1909.831 kb on - strand, within htpXat 1910.032 kb on + strand, within htpXat 1910.119 kb on + strand, within htpXat 1910.119 kb on + strand, within htpXat 1910.121 kb on + strand, within htpXat 1910.143 kb on - strand, within htpXat 1910.228 kb on - strand, within htpXat 1910.486 kb on - strand, within htpXat 1910.486 kb on - strand, within htpXat 1910.624 kb on + strandat 1910.643 kb on + strandat 1910.686 kb on + strandat 1910.686 kb on + strandat 1910.877 kb on - strandat 1910.898 kb on - strandat 1911.010 kb on - strandat 1911.068 kb on + strand, within prcat 1911.197 kb on - strand, within prcat 1911.283 kb on + strand, within prcat 1911.318 kb on + strand, within prcat 1911.341 kb on - strand, within prcat 1911.341 kb on - strand, within prcat 1911.400 kb on - strand, within prcat 1911.400 kb on - strand, within prcat 1911.511 kb on - strand, within prcat 1911.511 kb on - strand, within prcat 1911.530 kb on - strand, within prcat 1911.546 kb on - strand, within prcat 1911.546 kb on - strand, within prcat 1911.606 kb on + strand, within prcat 1911.651 kb on + strand, within prcat 1911.749 kb on - strand, within prcat 1911.749 kb on - strand, within prcat 1911.847 kb on + strand, within prcat 1911.847 kb on + strand, within prcat 1912.082 kb on + strand, within prcat 1912.123 kb on - strand, within prcat 1912.140 kb on + strand, within prcat 1912.140 kb on + strand, within prcat 1912.166 kb on + strand, within prcat 1912.166 kb on + strand, within prcat 1912.759 kb on + strandat 1912.759 kb on + strandat 1912.794 kb on - strandat 1912.972 kb on + strand, within proQat 1912.972 kb on + strand, within proQat 1913.231 kb on - strand, within proQat 1913.263 kb on - strand, within proQat 1913.263 kb on - strand, within proQat 1913.405 kb on + strand, within proQat 1913.405 kb on + strand, within proQat 1913.413 kb on - strand, within proQat 1913.725 kb on - strand, within yebRat 1913.752 kb on + strand, within yebRat 1913.752 kb on + strand, within yebRat 1913.760 kb on - strand, within yebRat 1913.790 kb on - strand, within yebRat 1913.790 kb on - strand, within yebR

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + ciprofloxacin Time8hr
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1,909,831 - htpX b1829 0.13 +0.0
1,909,831 - htpX b1829 0.13 +0.0
1,910,032 + htpX b1829 0.35 +0.5
1,910,119 + htpX b1829 0.45 +0.3
1,910,119 + htpX b1829 0.45 -0.4
1,910,121 + htpX b1829 0.46 +0.1
1,910,143 - htpX b1829 0.48 -0.2
1,910,228 - htpX b1829 0.58 -0.0
1,910,486 - htpX b1829 0.87 +0.4
1,910,486 - htpX b1829 0.87 -0.2
1,910,624 + +0.2
1,910,643 + +0.4
1,910,686 + +0.5
1,910,686 + +0.4
1,910,877 - +0.8
1,910,898 - +1.6
1,911,010 - +0.5
1,911,068 + prc b1830 0.13 +0.5
1,911,197 - prc b1830 0.20 +0.8
1,911,283 + prc b1830 0.24 +0.9
1,911,318 + prc b1830 0.26 +0.7
1,911,341 - prc b1830 0.27 +2.6
1,911,341 - prc b1830 0.27 +1.4
1,911,400 - prc b1830 0.30 -0.4
1,911,400 - prc b1830 0.30 +1.0
1,911,511 - prc b1830 0.35 +0.9
1,911,511 - prc b1830 0.35 +0.5
1,911,530 - prc b1830 0.36 +0.6
1,911,546 - prc b1830 0.37 +0.3
1,911,546 - prc b1830 0.37 +0.3
1,911,606 + prc b1830 0.40 +0.8
1,911,651 + prc b1830 0.42 +0.2
1,911,749 - prc b1830 0.47 +0.2
1,911,749 - prc b1830 0.47 +0.7
1,911,847 + prc b1830 0.51 +1.0
1,911,847 + prc b1830 0.51 +0.2
1,912,082 + prc b1830 0.63 +1.6
1,912,123 - prc b1830 0.65 +0.2
1,912,140 + prc b1830 0.66 -0.1
1,912,140 + prc b1830 0.66 +0.5
1,912,166 + prc b1830 0.67 +0.8
1,912,166 + prc b1830 0.67 +0.8
1,912,759 + +1.2
1,912,759 + +1.0
1,912,794 - +0.2
1,912,972 + proQ b1831 0.16 +1.3
1,912,972 + proQ b1831 0.16 +2.2
1,913,231 - proQ b1831 0.53 +0.9
1,913,263 - proQ b1831 0.58 +0.9
1,913,263 - proQ b1831 0.58 +0.4
1,913,405 + proQ b1831 0.78 +2.6
1,913,405 + proQ b1831 0.78 -0.0
1,913,413 - proQ b1831 0.79 +0.7
1,913,725 - yebR b1832 0.14 +0.3
1,913,752 + yebR b1832 0.19 -0.0
1,913,752 + yebR b1832 0.19 -0.7
1,913,760 - yebR b1832 0.21 -0.1
1,913,790 - yebR b1832 0.27 +0.1
1,913,790 - yebR b1832 0.27 +0.1

Or see this region's nucleotide sequence