Experiment: LB + ciprofloxacin Time8hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydeA and marC are separated by 24 nucleotides marC and marR are separated by 211 nucleotides marR and marA are separated by 19 nucleotides marA and marB are separated by 31 nucleotides marB and ydeD are separated by 30 nucleotides
b1528: ydeA - sugar efflux transporter (NCBI), at 1,615,052 to 1,616,242
ydeA
b1529: marC - predicted transporter (NCBI), at 1,616,267 to 1,616,932
marC
b1530: marR - multiple antibiotic resistance protein; repressor of mar operon (VIMSS), at 1,617,144 to 1,617,578
marR
b1531: marA - multiple antibiotic resistance; transcriptional activator of defense systems (VIMSS), at 1,617,598 to 1,617,981
marA
b1532: marB - hypothetical protein (NCBI), at 1,618,013 to 1,618,231
marB
b1533: ydeD - orf, hypothetical protein (VIMSS), at 1,618,262 to 1,619,161
ydeD
Position (kb)
1617
1618 Strain fitness (log2 ratio)
-2
-1
0
1 at 1616.153 kb on - strand at 1616.277 kb on + strand at 1616.319 kb on + strand at 1616.319 kb on + strand at 1616.522 kb on + strand, within marC at 1616.899 kb on + strand at 1617.172 kb on - strand at 1617.277 kb on - strand, within marR at 1617.288 kb on + strand, within marR at 1617.291 kb on - strand, within marR at 1617.291 kb on - strand, within marR at 1617.302 kb on + strand, within marR at 1617.312 kb on - strand, within marR at 1617.358 kb on + strand, within marR at 1617.377 kb on + strand, within marR at 1617.377 kb on + strand, within marR at 1617.471 kb on - strand, within marR at 1617.606 kb on + strand at 1617.638 kb on + strand, within marA at 1617.655 kb on - strand, within marA at 1617.702 kb on + strand, within marA at 1618.111 kb on - strand, within marB at 1618.215 kb on - strand at 1618.234 kb on - strand at 1618.521 kb on - strand, within ydeD at 1618.521 kb on - strand, within ydeD
Per-strain Table
Position Strand Gene LocusTag Fraction LB + ciprofloxacin Time8hr remove 1,616,153 - +0.5 1,616,277 + +1.6 1,616,319 + +0.2 1,616,319 + -0.4 1,616,522 + marC b1529 0.38 +0.0 1,616,899 + +1.5 1,617,172 - +0.2 1,617,277 - marR b1530 0.31 +1.0 1,617,288 + marR b1530 0.33 +0.2 1,617,291 - marR b1530 0.34 +0.3 1,617,291 - marR b1530 0.34 +0.1 1,617,302 + marR b1530 0.36 +0.3 1,617,312 - marR b1530 0.39 +0.8 1,617,358 + marR b1530 0.49 -0.2 1,617,377 + marR b1530 0.54 -0.0 1,617,377 + marR b1530 0.54 +1.4 1,617,471 - marR b1530 0.75 -2.5 1,617,606 + +0.2 1,617,638 + marA b1531 0.10 -0.3 1,617,655 - marA b1531 0.15 +0.5 1,617,702 + marA b1531 0.27 +0.2 1,618,111 - marB b1532 0.45 +0.0 1,618,215 - +0.3 1,618,234 - +0.3 1,618,521 - ydeD b1533 0.29 +0.2 1,618,521 - ydeD b1533 0.29 +0.1
Or see this region's nucleotide sequence