Strain Fitness in Escherichia coli BW25113 around b1176

Experiment: LB + ciprofloxacin Time8hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntminE and minD are separated by 3 nucleotidesminD and minC are separated by 23 nucleotidesminC and ycgJ are separated by 519 nucleotidesycgJ and ycgK are separated by 102 nucleotides b1174: minE - cell division topological specificity factor MinE (NCBI), at 1,223,502 to 1,223,768 minE b1175: minD - membrane ATPase of the MinC-MinD-MinE system (NCBI), at 1,223,772 to 1,224,584 minD b1176: minC - septum formation inhibitor (NCBI), at 1,224,608 to 1,225,303 minC b1177: ycgJ - hypothetical protein (NCBI), at 1,225,823 to 1,226,191 ycgJ b1178: ycgK - hypothetical protein (NCBI), at 1,226,294 to 1,226,695 ycgK Position (kb) 1224 1225 1226Strain fitness (log2 ratio) -1 0 1 2at 1223.902 kb on - strand, within minDat 1223.936 kb on - strand, within minDat 1223.941 kb on - strand, within minDat 1223.941 kb on - strand, within minDat 1224.022 kb on - strand, within minDat 1224.061 kb on + strand, within minDat 1224.461 kb on - strand, within minDat 1224.461 kb on - strand, within minDat 1224.509 kb on - strandat 1224.534 kb on + strandat 1224.577 kb on - strandat 1224.684 kb on + strand, within minCat 1224.784 kb on + strand, within minCat 1224.854 kb on - strand, within minCat 1225.133 kb on - strand, within minCat 1225.151 kb on - strand, within minCat 1225.151 kb on - strand, within minCat 1225.310 kb on - strandat 1225.310 kb on - strandat 1225.329 kb on + strandat 1225.354 kb on - strandat 1225.380 kb on - strandat 1225.382 kb on + strandat 1225.405 kb on - strandat 1225.606 kb on + strandat 1225.672 kb on - strandat 1225.677 kb on + strandat 1225.677 kb on + strandat 1225.688 kb on + strandat 1225.688 kb on + strandat 1225.692 kb on + strandat 1225.693 kb on - strandat 1225.700 kb on - strandat 1225.700 kb on - strandat 1225.712 kb on + strandat 1225.720 kb on + strandat 1225.720 kb on + strandat 1225.720 kb on + strandat 1225.728 kb on - strandat 1225.734 kb on + strandat 1225.734 kb on + strandat 1225.734 kb on + strandat 1225.742 kb on - strandat 1225.742 kb on - strandat 1225.744 kb on + strandat 1225.776 kb on - strandat 1225.794 kb on - strandat 1225.820 kb on + strandat 1225.865 kb on + strand, within ycgJat 1225.865 kb on + strand, within ycgJat 1225.865 kb on + strand, within ycgJat 1225.893 kb on - strand, within ycgJat 1225.916 kb on - strand, within ycgJat 1225.916 kb on - strand, within ycgJat 1225.951 kb on - strand, within ycgJat 1226.030 kb on + strand, within ycgJat 1226.037 kb on + strand, within ycgJat 1226.037 kb on + strand, within ycgJat 1226.217 kb on - strandat 1226.219 kb on + strandat 1226.219 kb on + strandat 1226.232 kb on + strandat 1226.234 kb on + strandat 1226.237 kb on + strandat 1226.237 kb on + strandat 1226.240 kb on + strandat 1226.240 kb on + strandat 1226.240 kb on + strandat 1226.240 kb on + strandat 1226.240 kb on - strandat 1226.243 kb on - strandat 1226.243 kb on - strandat 1226.244 kb on + strandat 1226.248 kb on - strandat 1226.276 kb on + strandat 1226.284 kb on - strandat 1226.284 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + ciprofloxacin Time8hr
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1,223,902 - minD b1175 0.16 +0.3
1,223,936 - minD b1175 0.20 +0.2
1,223,941 - minD b1175 0.21 +1.2
1,223,941 - minD b1175 0.21 -0.2
1,224,022 - minD b1175 0.31 +0.4
1,224,061 + minD b1175 0.36 +0.8
1,224,461 - minD b1175 0.85 -0.2
1,224,461 - minD b1175 0.85 -0.5
1,224,509 - +0.4
1,224,534 + +1.5
1,224,577 - +0.2
1,224,684 + minC b1176 0.11 +1.1
1,224,784 + minC b1176 0.25 +0.8
1,224,854 - minC b1176 0.35 -0.0
1,225,133 - minC b1176 0.75 +0.8
1,225,151 - minC b1176 0.78 +1.7
1,225,151 - minC b1176 0.78 +1.4
1,225,310 - -0.1
1,225,310 - +0.8
1,225,329 + +0.8
1,225,354 - -0.1
1,225,380 - +0.2
1,225,382 + +1.5
1,225,405 - +0.5
1,225,606 + +0.0
1,225,672 - -0.2
1,225,677 + +0.1
1,225,677 + +0.6
1,225,688 + +0.1
1,225,688 + +0.3
1,225,692 + +0.2
1,225,693 - +0.1
1,225,700 - +0.3
1,225,700 - +0.2
1,225,712 + +0.0
1,225,720 + +0.3
1,225,720 + -0.3
1,225,720 + +0.3
1,225,728 - +0.3
1,225,734 + +0.5
1,225,734 + +0.4
1,225,734 + +0.1
1,225,742 - -1.2
1,225,742 - +0.6
1,225,744 + +0.4
1,225,776 - -0.6
1,225,794 - -0.2
1,225,820 + +0.1
1,225,865 + ycgJ b1177 0.11 -0.0
1,225,865 + ycgJ b1177 0.11 +0.2
1,225,865 + ycgJ b1177 0.11 +0.0
1,225,893 - ycgJ b1177 0.19 +2.1
1,225,916 - ycgJ b1177 0.25 +0.5
1,225,916 - ycgJ b1177 0.25 +0.5
1,225,951 - ycgJ b1177 0.35 +0.5
1,226,030 + ycgJ b1177 0.56 +0.3
1,226,037 + ycgJ b1177 0.58 -0.2
1,226,037 + ycgJ b1177 0.58 +1.0
1,226,217 - +0.5
1,226,219 + -0.2
1,226,219 + +0.8
1,226,232 + +1.0
1,226,234 + -0.1
1,226,237 + +0.8
1,226,237 + +0.3
1,226,240 + +1.3
1,226,240 + +0.1
1,226,240 + +0.5
1,226,240 + +0.2
1,226,240 - +0.5
1,226,243 - +0.5
1,226,243 - +0.4
1,226,244 + +0.2
1,226,248 - +0.5
1,226,276 + -1.3
1,226,284 - -0.5
1,226,284 - -1.7

Or see this region's nucleotide sequence