Strain Fitness in Escherichia coli BW25113 around b3836

Experiment: LB + ciprofloxacin Time6hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntubiB and b3836 are separated by 78 nucleotidesb3836 and tatB are separated by 3 nucleotidestatB and tatC are separated by 2 nucleotides b3835: ubiB - putative ubiquinone biosynthesis protein UbiB (NCBI), at 4,018,249 to 4,019,889 ubiB b3836: b3836 - orf, hypothetical protein (VIMSS), at 4,019,968 to 4,020,237 b3836 b3838: tatB - sec-independent translocase (NCBI), at 4,020,241 to 4,020,756 tatB b3839: tatC - TatABCE protein translocation system subunit (NCBI), at 4,020,759 to 4,021,535 tatC Position (kb) 4019 4020 4021Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 4019.914 kb on + strandat 4019.914 kb on + strandat 4019.957 kb on - strandat 4019.960 kb on - strandat 4019.972 kb on - strandat 4019.975 kb on + strandat 4019.975 kb on + strandat 4019.981 kb on + strandat 4019.983 kb on - strandat 4019.991 kb on + strandat 4020.007 kb on - strand, within b3836at 4020.011 kb on + strand, within b3836at 4020.014 kb on + strand, within b3836at 4020.181 kb on + strand, within b3836at 4020.182 kb on + strand, within b3836at 4020.182 kb on + strand, within b3836at 4020.197 kb on + strand, within b3836at 4020.224 kb on - strandat 4020.226 kb on - strandat 4020.229 kb on + strandat 4020.232 kb on + strandat 4020.239 kb on - strandat 4020.262 kb on - strandat 4020.276 kb on + strandat 4020.282 kb on - strandat 4020.353 kb on - strand, within tatBat 4020.357 kb on + strand, within tatBat 4020.357 kb on + strand, within tatBat 4020.366 kb on + strand, within tatBat 4020.366 kb on + strand, within tatBat 4020.366 kb on + strand, within tatBat 4020.366 kb on + strand, within tatBat 4020.368 kb on + strand, within tatBat 4020.376 kb on + strand, within tatBat 4020.381 kb on + strand, within tatBat 4020.381 kb on + strand, within tatBat 4020.381 kb on + strand, within tatBat 4020.381 kb on + strand, within tatBat 4020.384 kb on - strand, within tatBat 4020.423 kb on - strand, within tatBat 4020.423 kb on - strand, within tatBat 4020.425 kb on + strand, within tatBat 4020.453 kb on + strand, within tatBat 4020.490 kb on + strand, within tatBat 4020.491 kb on + strand, within tatBat 4020.491 kb on + strand, within tatBat 4020.495 kb on + strand, within tatBat 4020.495 kb on + strand, within tatBat 4020.522 kb on + strand, within tatBat 4020.527 kb on - strand, within tatBat 4020.539 kb on + strand, within tatBat 4020.572 kb on + strand, within tatBat 4020.579 kb on - strand, within tatBat 4020.596 kb on + strand, within tatBat 4020.640 kb on - strand, within tatBat 4020.645 kb on - strand, within tatBat 4020.686 kb on + strand, within tatBat 4020.727 kb on - strandat 4020.750 kb on + strandat 4020.771 kb on - strandat 4020.777 kb on - strandat 4020.782 kb on + strandat 4020.796 kb on + strandat 4020.804 kb on - strandat 4020.814 kb on - strandat 4020.814 kb on - strandat 4020.814 kb on - strandat 4020.824 kb on - strandat 4020.839 kb on + strand, within tatCat 4020.845 kb on + strand, within tatCat 4020.851 kb on - strand, within tatCat 4020.881 kb on + strand, within tatCat 4020.891 kb on + strand, within tatCat 4020.891 kb on + strand, within tatCat 4020.891 kb on + strand, within tatCat 4020.891 kb on - strand, within tatCat 4020.902 kb on - strand, within tatCat 4020.910 kb on + strand, within tatCat 4020.910 kb on + strand, within tatCat 4020.933 kb on - strand, within tatCat 4020.990 kb on - strand, within tatCat 4021.018 kb on + strand, within tatCat 4021.028 kb on + strand, within tatCat 4021.028 kb on + strand, within tatCat 4021.030 kb on - strand, within tatCat 4021.030 kb on - strand, within tatCat 4021.045 kb on + strand, within tatCat 4021.053 kb on + strand, within tatCat 4021.053 kb on + strand, within tatCat 4021.053 kb on + strand, within tatCat 4021.055 kb on + strand, within tatCat 4021.055 kb on + strand, within tatCat 4021.055 kb on + strand, within tatCat 4021.063 kb on - strand, within tatCat 4021.063 kb on - strand, within tatCat 4021.063 kb on - strand, within tatCat 4021.063 kb on - strand, within tatCat 4021.070 kb on - strand, within tatCat 4021.078 kb on - strand, within tatCat 4021.109 kb on - strand, within tatCat 4021.167 kb on + strand, within tatCat 4021.167 kb on + strand, within tatCat 4021.169 kb on + strand, within tatCat 4021.169 kb on + strand, within tatCat 4021.177 kb on - strand, within tatCat 4021.205 kb on - strand, within tatCat 4021.228 kb on + strand, within tatCat 4021.228 kb on + strand, within tatC

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + ciprofloxacin Time6hr
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4,019,914 + +0.4
4,019,914 + +0.4
4,019,957 - +0.7
4,019,960 - +0.6
4,019,972 - +0.3
4,019,975 + +0.5
4,019,975 + +0.2
4,019,981 + +0.4
4,019,983 - -0.9
4,019,991 + +0.7
4,020,007 - b3836 b3836 0.14 +0.3
4,020,011 + b3836 b3836 0.16 +0.3
4,020,014 + b3836 b3836 0.17 +0.5
4,020,181 + b3836 b3836 0.79 +0.6
4,020,182 + b3836 b3836 0.79 +0.6
4,020,182 + b3836 b3836 0.79 +0.7
4,020,197 + b3836 b3836 0.85 -0.0
4,020,224 - +0.2
4,020,226 - +0.1
4,020,229 + +0.5
4,020,232 + -0.3
4,020,239 - +0.4
4,020,262 - +0.4
4,020,276 + +1.3
4,020,282 - +0.2
4,020,353 - tatB b3838 0.22 -0.0
4,020,357 + tatB b3838 0.22 -1.0
4,020,357 + tatB b3838 0.22 +0.8
4,020,366 + tatB b3838 0.24 -0.3
4,020,366 + tatB b3838 0.24 +0.2
4,020,366 + tatB b3838 0.24 +0.8
4,020,366 + tatB b3838 0.24 -1.4
4,020,368 + tatB b3838 0.25 +0.8
4,020,376 + tatB b3838 0.26 -1.1
4,020,381 + tatB b3838 0.27 -0.3
4,020,381 + tatB b3838 0.27 -1.0
4,020,381 + tatB b3838 0.27 +0.6
4,020,381 + tatB b3838 0.27 +1.6
4,020,384 - tatB b3838 0.28 +1.0
4,020,423 - tatB b3838 0.35 +0.0
4,020,423 - tatB b3838 0.35 +1.6
4,020,425 + tatB b3838 0.36 +1.5
4,020,453 + tatB b3838 0.41 +0.2
4,020,490 + tatB b3838 0.48 +0.3
4,020,491 + tatB b3838 0.48 -0.8
4,020,491 + tatB b3838 0.48 +0.9
4,020,495 + tatB b3838 0.49 +0.4
4,020,495 + tatB b3838 0.49 -0.7
4,020,522 + tatB b3838 0.54 +0.2
4,020,527 - tatB b3838 0.55 +1.6
4,020,539 + tatB b3838 0.58 +0.3
4,020,572 + tatB b3838 0.64 +0.1
4,020,579 - tatB b3838 0.66 -0.2
4,020,596 + tatB b3838 0.69 +0.5
4,020,640 - tatB b3838 0.77 -0.5
4,020,645 - tatB b3838 0.78 +0.8
4,020,686 + tatB b3838 0.86 +0.4
4,020,727 - -0.2
4,020,750 + +0.6
4,020,771 - -1.8
4,020,777 - +1.0
4,020,782 + +0.1
4,020,796 + +0.9
4,020,804 - +1.2
4,020,814 - -1.6
4,020,814 - -2.3
4,020,814 - +1.6
4,020,824 - +0.1
4,020,839 + tatC b3839 0.10 +0.9
4,020,845 + tatC b3839 0.11 +0.7
4,020,851 - tatC b3839 0.12 +1.3
4,020,881 + tatC b3839 0.16 -0.7
4,020,891 + tatC b3839 0.17 +2.2
4,020,891 + tatC b3839 0.17 +0.0
4,020,891 + tatC b3839 0.17 -0.4
4,020,891 - tatC b3839 0.17 +1.6
4,020,902 - tatC b3839 0.18 -0.1
4,020,910 + tatC b3839 0.19 -0.7
4,020,910 + tatC b3839 0.19 +0.1
4,020,933 - tatC b3839 0.22 +0.3
4,020,990 - tatC b3839 0.30 -0.7
4,021,018 + tatC b3839 0.33 +0.0
4,021,028 + tatC b3839 0.35 -1.0
4,021,028 + tatC b3839 0.35 +0.0
4,021,030 - tatC b3839 0.35 -0.3
4,021,030 - tatC b3839 0.35 +0.0
4,021,045 + tatC b3839 0.37 +0.9
4,021,053 + tatC b3839 0.38 -0.3
4,021,053 + tatC b3839 0.38 -0.7
4,021,053 + tatC b3839 0.38 -1.2
4,021,055 + tatC b3839 0.38 -1.0
4,021,055 + tatC b3839 0.38 +0.1
4,021,055 + tatC b3839 0.38 +4.4
4,021,063 - tatC b3839 0.39 +0.6
4,021,063 - tatC b3839 0.39 +0.0
4,021,063 - tatC b3839 0.39 +0.3
4,021,063 - tatC b3839 0.39 +0.3
4,021,070 - tatC b3839 0.40 +0.9
4,021,078 - tatC b3839 0.41 +0.0
4,021,109 - tatC b3839 0.45 +1.2
4,021,167 + tatC b3839 0.53 +0.6
4,021,167 + tatC b3839 0.53 +3.2
4,021,169 + tatC b3839 0.53 -0.4
4,021,169 + tatC b3839 0.53 +1.6
4,021,177 - tatC b3839 0.54 +0.9
4,021,205 - tatC b3839 0.57 +0.6
4,021,228 + tatC b3839 0.60 +0.6
4,021,228 + tatC b3839 0.60 -0.7

Or see this region's nucleotide sequence