Strain Fitness in Escherichia coli BW25113 around b2968

Experiment: LB + ciprofloxacin Time6hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyqgA and pheV are separated by 105 nucleotidespheV and yghD are separated by 148 nucleotidesyghD and yghE are separated by 1 nucleotidesyghE and yghF are separated by 2 nucleotides b2966: yqgA - predicted inner membrane protein (NCBI), at 3,107,575 to 3,108,282 yqgA b2967: pheV - tRNA-Phe (NCBI), at 3,108,388 to 3,108,463 pheV b2968: yghD - predicted secretion pathway M-type protein, membrane anchored (NCBI), at 3,108,612 to 3,109,148 yghD b2969: yghE - predicted secretion pathway protein, L-type protein (pseudogene) (RefSeq), at 3,109,150 to 3,110,112 yghE b2970: yghF - predicted secretion pathway protein, C-type protein (NCBI), at 3,110,115 to 3,110,963 yghF Position (kb) 3108 3109 3110Strain fitness (log2 ratio) -1 0 1at 3107.616 kb on - strandat 3107.895 kb on + strand, within yqgAat 3107.918 kb on - strand, within yqgAat 3107.932 kb on - strand, within yqgAat 3108.033 kb on + strand, within yqgAat 3108.117 kb on + strand, within yqgAat 3108.160 kb on + strand, within yqgAat 3108.181 kb on - strand, within yqgAat 3108.221 kb on - strandat 3108.222 kb on + strandat 3108.222 kb on + strandat 3108.378 kb on - strandat 3108.378 kb on - strandat 3108.593 kb on + strandat 3108.629 kb on - strandat 3108.629 kb on - strandat 3108.649 kb on + strandat 3108.766 kb on + strand, within yghDat 3108.821 kb on + strand, within yghDat 3108.833 kb on - strand, within yghDat 3108.833 kb on - strand, within yghDat 3108.878 kb on + strand, within yghDat 3108.931 kb on - strand, within yghDat 3108.940 kb on + strand, within yghDat 3108.940 kb on + strand, within yghDat 3109.078 kb on - strand, within yghDat 3109.098 kb on - strandat 3109.123 kb on - strandat 3109.174 kb on + strandat 3109.220 kb on - strandat 3109.437 kb on + strand, within yghEat 3109.538 kb on + strand, within yghEat 3109.551 kb on + strand, within yghEat 3109.704 kb on + strand, within yghEat 3109.704 kb on + strand, within yghEat 3109.712 kb on - strand, within yghEat 3109.886 kb on - strand, within yghEat 3109.933 kb on - strand, within yghEat 3110.044 kb on - strandat 3110.073 kb on + strandat 3110.073 kb on + strandat 3110.085 kb on - strandat 3110.085 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + ciprofloxacin Time6hr
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3,107,616 - -0.0
3,107,895 + yqgA b2966 0.45 +0.8
3,107,918 - yqgA b2966 0.48 -0.5
3,107,932 - yqgA b2966 0.50 -0.3
3,108,033 + yqgA b2966 0.65 +0.5
3,108,117 + yqgA b2966 0.77 +0.0
3,108,160 + yqgA b2966 0.83 +0.2
3,108,181 - yqgA b2966 0.86 +0.1
3,108,221 - +0.2
3,108,222 + -0.0
3,108,222 + +0.1
3,108,378 - +0.4
3,108,378 - -0.2
3,108,593 + -0.3
3,108,629 - -0.4
3,108,629 - +0.3
3,108,649 + -0.2
3,108,766 + yghD b2968 0.29 +0.7
3,108,821 + yghD b2968 0.39 +0.4
3,108,833 - yghD b2968 0.41 +0.2
3,108,833 - yghD b2968 0.41 +0.4
3,108,878 + yghD b2968 0.50 +0.3
3,108,931 - yghD b2968 0.59 -0.1
3,108,940 + yghD b2968 0.61 +0.8
3,108,940 + yghD b2968 0.61 +0.6
3,109,078 - yghD b2968 0.87 +0.2
3,109,098 - +0.2
3,109,123 - +0.4
3,109,174 + +0.3
3,109,220 - +0.4
3,109,437 + yghE b2969 0.30 -0.3
3,109,538 + yghE b2969 0.40 +0.2
3,109,551 + yghE b2969 0.42 +0.1
3,109,704 + yghE b2969 0.58 -0.0
3,109,704 + yghE b2969 0.58 -0.1
3,109,712 - yghE b2969 0.58 +0.5
3,109,886 - yghE b2969 0.76 +0.0
3,109,933 - yghE b2969 0.81 +0.4
3,110,044 - +0.0
3,110,073 + +0.0
3,110,073 + +0.0
3,110,085 - +0.2
3,110,085 - +0.1

Or see this region's nucleotide sequence