Strain Fitness in Escherichia coli BW25113 around b1427

Experiment: LB + ciprofloxacin Time6hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmdoD and ydcH are separated by 139 nucleotidesydcH and rimL are separated by 62 nucleotidesrimL and ydcK overlap by 9 nucleotides b1424: mdoD - glucan biosynthesis protein, periplasmic (RefSeq), at 1,494,880 to 1,496,535 mdoD b1426: ydcH - orf, hypothetical protein (VIMSS), at 1,496,675 to 1,496,899 ydcH b1427: rimL - ribosomal-protein-L7/L12-serine acetyltransferase (NCBI), at 1,496,962 to 1,497,501 rimL b1428: ydcK - predicted enzyme (NCBI), at 1,497,493 to 1,498,473 ydcK Position (kb) 1496 1497 1498Strain fitness (log2 ratio) -2 -1 0 1at 1496.356 kb on - strand, within mdoDat 1496.356 kb on - strand, within mdoDat 1496.405 kb on + strandat 1496.405 kb on + strandat 1496.405 kb on + strandat 1496.413 kb on - strandat 1496.413 kb on - strandat 1496.413 kb on - strandat 1496.669 kb on - strandat 1496.669 kb on - strandat 1496.673 kb on - strandat 1496.808 kb on - strand, within ydcHat 1496.896 kb on - strandat 1497.142 kb on - strand, within rimLat 1497.231 kb on + strand, within rimLat 1497.266 kb on + strand, within rimLat 1497.328 kb on - strand, within rimLat 1497.407 kb on + strand, within rimLat 1497.636 kb on + strand, within ydcKat 1497.662 kb on + strand, within ydcKat 1497.751 kb on - strand, within ydcKat 1497.751 kb on - strand, within ydcKat 1497.751 kb on - strand, within ydcKat 1497.765 kb on + strand, within ydcKat 1497.766 kb on + strand, within ydcKat 1497.792 kb on - strand, within ydcKat 1497.805 kb on + strand, within ydcKat 1497.829 kb on - strand, within ydcKat 1498.038 kb on + strand, within ydcKat 1498.172 kb on + strand, within ydcKat 1498.172 kb on + strand, within ydcKat 1498.189 kb on - strand, within ydcKat 1498.189 kb on - strand, within ydcKat 1498.219 kb on - strand, within ydcKat 1498.471 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + ciprofloxacin Time6hr
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1,496,356 - mdoD b1424 0.89 +0.0
1,496,356 - mdoD b1424 0.89 +0.0
1,496,405 + +0.0
1,496,405 + +0.4
1,496,405 + -0.3
1,496,413 - +1.0
1,496,413 - +0.3
1,496,413 - -0.8
1,496,669 - +0.7
1,496,669 - +0.4
1,496,673 - -0.1
1,496,808 - ydcH b1426 0.59 +0.3
1,496,896 - +1.0
1,497,142 - rimL b1427 0.33 +0.3
1,497,231 + rimL b1427 0.50 +0.2
1,497,266 + rimL b1427 0.56 +1.2
1,497,328 - rimL b1427 0.68 -0.0
1,497,407 + rimL b1427 0.82 +0.2
1,497,636 + ydcK b1428 0.15 -0.8
1,497,662 + ydcK b1428 0.17 +0.5
1,497,751 - ydcK b1428 0.26 -0.1
1,497,751 - ydcK b1428 0.26 +0.4
1,497,751 - ydcK b1428 0.26 +0.3
1,497,765 + ydcK b1428 0.28 -0.0
1,497,766 + ydcK b1428 0.28 -0.1
1,497,792 - ydcK b1428 0.30 +0.2
1,497,805 + ydcK b1428 0.32 -0.1
1,497,829 - ydcK b1428 0.34 -0.5
1,498,038 + ydcK b1428 0.56 +0.5
1,498,172 + ydcK b1428 0.69 -1.8
1,498,172 + ydcK b1428 0.69 -1.0
1,498,189 - ydcK b1428 0.71 -0.1
1,498,189 - ydcK b1428 0.71 -0.9
1,498,219 - ydcK b1428 0.74 -0.2
1,498,471 - +0.4

Or see this region's nucleotide sequence