Strain Fitness in Escherichia coli BW25113 around b1184

Experiment: LB + ciprofloxacin Time6hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthlyE and umuD are separated by 372 nucleotidesumuD and umuC overlap by 1 nucleotidesumuC and dsbB are separated by 45 nucleotidesdsbB and nhaB are separated by 145 nucleotides b1182: hlyE - hemolysin E (VIMSS), at 1,228,706 to 1,229,617 hlyE b1183: umuD - DNA polymerase V, subunit D (NCBI), at 1,229,990 to 1,230,409 umuD b1184: umuC - DNA polymerase V subunit UmuC (NCBI), at 1,230,409 to 1,231,677 umuC b1185: dsbB - reoxidizes DsbA protein following formation of disulfide bond in P-ring of flagella. (VIMSS), at 1,231,723 to 1,232,253 dsbB b1186: nhaB - NhaB sodium/proton transporter (Katherine Huang), at 1,232,399 to 1,233,940 nhaB Position (kb) 1230 1231 1232Strain fitness (log2 ratio) -1 0 1 2 3at 1229.669 kb on - strandat 1229.703 kb on - strandat 1229.704 kb on - strandat 1229.718 kb on - strandat 1229.718 kb on - strandat 1229.838 kb on - strandat 1229.840 kb on - strandat 1229.842 kb on - strandat 1229.842 kb on - strandat 1229.844 kb on - strandat 1229.851 kb on - strandat 1229.851 kb on - strandat 1229.853 kb on - strandat 1229.859 kb on - strandat 1229.859 kb on - strandat 1229.859 kb on - strandat 1229.861 kb on + strandat 1229.897 kb on - strandat 1229.897 kb on - strandat 1230.140 kb on + strand, within umuDat 1230.307 kb on + strand, within umuDat 1230.446 kb on + strandat 1230.893 kb on + strand, within umuCat 1230.956 kb on + strand, within umuCat 1231.013 kb on - strand, within umuCat 1231.029 kb on + strand, within umuCat 1231.038 kb on - strand, within umuCat 1231.109 kb on + strand, within umuCat 1231.109 kb on + strand, within umuCat 1231.208 kb on + strandat 1231.208 kb on + strand, within umuCat 1231.270 kb on + strand, within umuCat 1231.270 kb on + strand, within umuCat 1231.320 kb on + strand, within umuCat 1231.359 kb on + strand, within umuCat 1231.359 kb on + strand, within umuCat 1231.382 kb on - strand, within umuCat 1231.457 kb on + strand, within umuCat 1231.519 kb on - strand, within umuCat 1231.550 kb on + strand, within umuCat 1231.551 kb on - strandat 1231.553 kb on - strandat 1231.573 kb on - strandat 1231.597 kb on + strandat 1231.625 kb on + strandat 1231.627 kb on - strandat 1231.643 kb on - strandat 1231.645 kb on - strandat 1231.660 kb on - strandat 1231.961 kb on + strand, within dsbBat 1231.961 kb on + strand, within dsbBat 1231.979 kb on - strand, within dsbBat 1232.074 kb on + strand, within dsbBat 1232.074 kb on + strand, within dsbBat 1232.077 kb on - strand, within dsbBat 1232.272 kb on + strandat 1232.272 kb on + strandat 1232.504 kb on - strandat 1232.509 kb on - strandat 1232.525 kb on + strandat 1232.569 kb on - strand, within nhaB

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Per-strain Table

Position Strand Gene LocusTag Fraction LB + ciprofloxacin Time6hr
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1,229,669 - +0.8
1,229,703 - +3.0
1,229,704 - +1.1
1,229,718 - +0.8
1,229,718 - +0.8
1,229,838 - -0.7
1,229,840 - -0.2
1,229,842 - -0.1
1,229,842 - +1.0
1,229,844 - +0.7
1,229,851 - -0.3
1,229,851 - +0.6
1,229,853 - +0.3
1,229,859 - -0.3
1,229,859 - +0.2
1,229,859 - +0.1
1,229,861 + -0.8
1,229,897 - +0.6
1,229,897 - +0.4
1,230,140 + umuD b1183 0.36 +0.3
1,230,307 + umuD b1183 0.75 +0.2
1,230,446 + +0.2
1,230,893 + umuC b1184 0.38 +0.3
1,230,956 + umuC b1184 0.43 +0.4
1,231,013 - umuC b1184 0.48 +1.1
1,231,029 + umuC b1184 0.49 +0.3
1,231,038 - umuC b1184 0.50 +0.7
1,231,109 + umuC b1184 0.55 -0.2
1,231,109 + umuC b1184 0.55 -0.0
1,231,208 + +1.4
1,231,208 + umuC b1184 0.63 +0.3
1,231,270 + umuC b1184 0.68 +0.3
1,231,270 + umuC b1184 0.68 +0.4
1,231,320 + umuC b1184 0.72 +0.4
1,231,359 + umuC b1184 0.75 +0.5
1,231,359 + umuC b1184 0.75 +0.7
1,231,382 - umuC b1184 0.77 +0.1
1,231,457 + umuC b1184 0.83 +0.7
1,231,519 - umuC b1184 0.87 +0.7
1,231,550 + umuC b1184 0.90 +0.7
1,231,551 - +0.4
1,231,553 - +1.0
1,231,573 - -0.0
1,231,597 + +0.3
1,231,625 + +0.5
1,231,627 - +0.2
1,231,643 - +0.4
1,231,645 - -0.4
1,231,660 - +2.0
1,231,961 + dsbB b1185 0.45 +1.2
1,231,961 + dsbB b1185 0.45 +0.7
1,231,979 - dsbB b1185 0.48 +1.5
1,232,074 + dsbB b1185 0.66 +1.7
1,232,074 + dsbB b1185 0.66 +1.1
1,232,077 - dsbB b1185 0.67 +1.6
1,232,272 + +0.7
1,232,272 + -0.3
1,232,504 - +0.2
1,232,509 - -0.1
1,232,525 + -0.2
1,232,569 - nhaB b1186 0.11 -0.7

Or see this region's nucleotide sequence